Identification of IP-10 and IL-5 as Proteins Differentially Expressed in Human Complicated and Uncomplicated Carotid Atherosclerotic Plaques

Abstract

Inflammation plays a crucial role in the development and progression of atherosclerotic plaques. The aim of this study is to compare culture supernatants from uncomplicated and complicated carotid atherosclerotic plaques by a multiplex approach, to assess the molecular mediators associated with a plaque complicated phenotype. Atherosclerotic plaques were obtained from 17 patients undergoing carotid endarterectomy. Supernatants from plaque cultures were evaluated by Bio-Plex cytokine assay to determine 27 pro- and anti-inflammatory cytokines, chemokines and growth factors. Complicated plaques secreted higher levels of IP-10 (p = 0.027) and lower levels of IL-5 (p = 0.045) than did uncomplicated ones. Distinctive secretory patterns of cytokines, chemokines and growth factors were present in the two types of plaque. Our study identifies IP-10 and IL-5 as proteins differentiating complicated and uncomplicated plaques from human carotid arteries and provides new insights into the interplay of molecular mediators with atherosclerotic plaque progression.

Epigenetic Age Acceleration in Adolescence Associates With BMI, Inflammation, and Risk Score for Middle Age Cardiovascular Disease

“…Interferon-gamma induced protein of 10 kDa (IP-10) is a monocyte-derived pro-inflammatory chemokine that promotes the recruitment of lymphocytes and monocytes to sites of inflammation. IP10 is expressed in human atherosclerotic plaques(45) and plasma levels are elevated in patients with diabetes(46) and the metabolic syndrome(47). Interleukin-18 (IL-18) and its binding protein (IL-18BP) were also associated with EEAA .…”

Acute phase proteins and IP-10 as triage tests for the diagnosis of tuberculosis: systematic review and meta-analysis

“…Furthermore, although many studies have investigated IP-10 expression as a specific response to the in vitro stimulation of white blood cells to specific TB antigens, few studies have reported its use in its steady, unstimulated concentrations. Altered serum and/or tissue expression of IP-10 has been associated with inflammatory diseases, including organ-specific or systemic autoimmune diseases [35], neurologic disorders [36], vascular diseases [37,38] and viral and bacterial infections [39]. One previous meta-analysis [40] reported a pooled sensitivity and specificity of 73% and 82% for the diagnosis of TB.…”

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“…Interferon- γ -induced protein of 10 kDa (IP-10) is a pro-inflammatory chemokine generated by monocytes to promote the recruitment of lymphocytes and monocytes to sites of inflammation. It is expressed in human atherosclerotic plaques ( 80 ) and plasma levels have been correlated with waist circumference and BMI in adolescents ( 73 ) .…”