Identification of Interstitial Cells of Cajal in the Rabbit Portal Vein

Povstyan, Oleksandr V.; Gordienko, Dmitri; Harhun, Maksym I.; Bolton, T. B.

doi:10.1023/b:neph.0000008819.64724.4d

Cited by 17 publications

(59 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The adventitial cells were often in close apposition to VSMCs and may represent adventitial fibroblasts or vascular "interstitial cells of Cajal" (ICCs) (23,24). ICCs are smooth muscle/ fibroblast-like cells that are present in the gastrointestinal tract, urinary tract, mesenteric resistance arteries, and portal vein, where they are thought to function as pacemakers, regulating the phasic activity of smooth muscle cells (23,24).…”

Section: Discussionmentioning

confidence: 99%

Imaging remodeling of the actin cytoskeleton in vascular smooth muscle cells after mechanosensitive arteriolar constriction

Flavahan

Bailey

Flavahan

et al. 2005

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

Experiments were performed to determine whether remodeling of the actin cytoskeleton contributes to arteriolar constriction. Mouse tail arterioles were mounted on cannulae in a myograph and superfused with buffer solution. The α1-adrenergic agonist phenylephrine (0.1–1 μmol/l) caused constriction that was unaffected by cytochalasin D (300 nmol/l) or latrunculin A (100 nmol/l), inhibitors of actin polymerization. In contrast, each compound abolished the mechanosensitive constriction (myogenic response) evoked by elevation in transmural pressure (PTM; 10–60 or 90 mmHg). Arterioles were fixed, permeabilized, and stained with Alexa-568 phalloidin and Alexa-488 DNAse I to visualize F-actin and G-actin, respectively, using a Zeiss 510 laser scanning microscope. Elevation in PTM, but not phenylephrine (1 μmol/l), significantly increased the intensity of F-actin and significantly decreased the intensity of G-actin staining in arteriolar vascular smooth muscle cells (VSMCs). The increase in F-actin staining caused by an elevation in PTM was inhibited by cytochalasin D. In VSMCs at 10 mmHg, prominent F-actin staining was restricted to the cell periphery, whereas after elevation in PTM, transcytoplasmic F-actin fibers were localized through the cell interior, running parallel to the long axis of the cells. Phenylephrine (1 μmol/l) did not alter the architecture of the actin cytoskeleton. In contrast to VSMCs, the actin cytoskeleton of endothelial or adventitial cells was not altered by an elevation in PTM. Therefore, the actin cytoskeleton of VSMCs undergoes dramatic alteration after elevation in PTM of arterioles and plays a selective and essential role in mechanosensitive myogenic constriction.

show abstract

Section: Discussionmentioning

confidence: 99%

Imaging remodeling of the actin cytoskeleton in vascular smooth muscle cells after mechanosensitive arteriolar constriction

Flavahan

Bailey

Flavahan

et al. 2005

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

show abstract

“…Several studies have suggested that the differentiation of ICC is dependent on ENS (3) . After the description of ICC in the gut wall, the presence of ICC-like cells has been demonstrated in the smooth muscle layers of various organs, such as the human ureter (4) , rabbit portal vein (5) and pancreas (6) . For many years, no conclusive data were available about ICC being smooth muscle cells or fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

Is the positive c-kit immunostaining associated with the presence of cells analogous to the intersticial cells of Cajal in the ciliary muscle?

Paula¹,

Souza²,

Oliveira³

et al. 2009

Arq. Bras. Oftalmol.

View full text Add to dashboard Cite

“…Interstitial cells of Cajal (ICCs), first described more than 100 years ago and initially thought to be present only in the gastrointestinal tract, are now considered to be responsible for the generation and/or propagation of slow waves (Povstyan et al, 2003;Huang and Xu, 2010). It is now known that ICCs play important roles in the regulation of gastrointestinal motility.…”

mentioning

confidence: 99%

“…ICCs are known to be immunopositive for c-kit, a proto-oncogene that encodes a tyrosine kinase receptor (Mei et al, 2009), making it a useful marker for these cells (Ward and Sanders, 2001;Povstyan et al, 2003;Harhun et al, 2005;Sanders et al, 2006;Popescu and Faussone-Pellegrini, 2010). However, it is not mandatory for all ICCs to be labeled with c-kit antibodies (Huizinga and Faussone-Pellegrini, 2005).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Extrahepatic and Intrahepatic Human Portal Interstitial Cajal Cells

Rusu

Pop

Hostiuc³

et al. 2011

The Anatomical Record

View full text Add to dashboard Cite

Portal interstitial cells of Cajal (PICCs), acting as vascular pacemakers, were previously only identified in nonhumans. Moreover, there is no evidence available about the presence of such cells within the liver. The objective of the study is to evaluate whether or not PICCs are identifiable in humans and, if they are, whether or not they are following the scaffold of portal vein (PV) branches within the liver. We obtained extrahepatic PVs and liver samples from six adult human cadavers, negative for liver disease, in accordance with ethical rules. They were stained with hematoxylin-eosin (HE) and Giemsa, and then we performed immunohistochemistry on formalin-fixed paraffin-embedded specimens for CD117/c-kit, a marker of the Cajal's cells. Immune labeling was also performed for S-100 protein, desmin, glial fibrillary acidic protein (GFAP), neurofilaments, a-smooth muscle actin (a-SMA), and CD34. c-kit-Positive PICCs were identified within the extrahepatic PV, in portal spaces, and septa. On adjacent sections, these PICCs were negative for all the other antibodies used. In conclusion, our study confirms the presence of extrahepatic PICCs on humans, which may act as a possible intrinsic pacemaker in the human PV. However, the intrahepatic PICCs, which were evidenced here for the first time, are in need for further experimental studies to evaluate their functional role. A promising further direction of the study is the PICCs role in the idiopathic portal hypertension. Anat Rec, 294:1382Rec, 294: -1392Rec, 294: , 2011. V V C 2011 Wiley-Liss, Inc.

show abstract

Identification of Interstitial Cells of Cajal in the Rabbit Portal Vein

Cited by 17 publications

References 0 publications

Imaging remodeling of the actin cytoskeleton in vascular smooth muscle cells after mechanosensitive arteriolar constriction

Imaging remodeling of the actin cytoskeleton in vascular smooth muscle cells after mechanosensitive arteriolar constriction

Is the positive c-kit immunostaining associated with the presence of cells analogous to the intersticial cells of Cajal in the ciliary muscle?

Extrahepatic and Intrahepatic Human Portal Interstitial Cajal Cells

Contact Info

Product

Resources

About