“…These methods have thus been widely used to study plant response to both biotic and abiotic stresses in many species although they mainly focused on non-legumes such as A. thaliana and rice (Cánovas et al, 2004). In legumes, these methods have been initially applied to establish the reference protein maps and their Downloaded by [West Virginia University] at 06:40 20 November 2014 subcellular localization (Watson et al, 2003;Komatsu and Ahsan, 2009;Katam et al, 2010;Lee et al, 2013a), the protein content of seed and their modification during seed development (Komatsu and Ahsan, 2009;Thompson et al, 2009;NautrupPedersen et al, 2010) and to study the symbiotic interaction with both rhizobium and mycorrhizal fungi (Mathesius, 2009;Recorbet et al, 2010;Salavati et al, 2012). The plant responses to abiotic stresses such as drought or salinity have also been widely studied at the proteomic level in legumes including M. truncatula, common bean, soybean, pea, peanut, grass pea and lupin (Kav et al, 2004;Pinheiro et al, 2005;Jain et al, 2006;Kottapalli et al, 2009;Chattopadhyay et al, 2011;Hakeem et al, 2012;Mohammadi et al, 2012;Staudinger et al, 2012;Komatsu et al, 2013;Subba et al, 2013;Zadraznik et al, 2013).…”