Identification of in planta-expressed arbuscular mycorrhizal fungal proteins upon comparison of the root proteomes of Medicago truncatula colonised with two Glomus species

Recorbet, Ghislaine; Valot, Benoı̂t; Robert, Franck; Gianinazzi‐Pearson, Vivienne; Dumas‐Gaudot, Eliane

doi:10.1016/j.fgb.2010.03.003

Cited by 18 publications

(11 citation statements)

References 67 publications

(96 reference statements)

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“…These methods have thus been widely used to study plant response to both biotic and abiotic stresses in many species although they mainly focused on non-legumes such as A. thaliana and rice (Cánovas et al, 2004). In legumes, these methods have been initially applied to establish the reference protein maps and their Downloaded by [West Virginia University] at 06:40 20 November 2014 subcellular localization (Watson et al, 2003;Komatsu and Ahsan, 2009;Katam et al, 2010;Lee et al, 2013a), the protein content of seed and their modification during seed development (Komatsu and Ahsan, 2009;Thompson et al, 2009;NautrupPedersen et al, 2010) and to study the symbiotic interaction with both rhizobium and mycorrhizal fungi (Mathesius, 2009;Recorbet et al, 2010;Salavati et al, 2012). The plant responses to abiotic stresses such as drought or salinity have also been widely studied at the proteomic level in legumes including M. truncatula, common bean, soybean, pea, peanut, grass pea and lupin (Kav et al, 2004;Pinheiro et al, 2005;Jain et al, 2006;Kottapalli et al, 2009;Chattopadhyay et al, 2011;Hakeem et al, 2012;Mohammadi et al, 2012;Staudinger et al, 2012;Komatsu et al, 2013;Subba et al, 2013;Zadraznik et al, 2013).…”

Section: Proteomicsmentioning

confidence: 99%

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Rubiales

Fondevilla

Cao

et al. 2014

Critical Reviews in Plant Sciences

176

132

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Section: Proteomicsmentioning

confidence: 99%

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Rubiales

Fondevilla

Cao

et al. 2014

Critical Reviews in Plant Sciences

176

132

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“…Analysis by Reverse Trancriptase PCR (RT-PCR) indicated direct correlation between transcript amounts of two genes and the accumulation of the correspondent proteins, which indicates that this regulation may happen at the transcription level [54]. Later, tandem mass spectrometry sequencing of proteins induced by inoculation of M. truncatula by either G. mosseae or G. intraradices allowed for the identification of eleven proteins of fungal origin into the root system, the first insight into fungal proteins involved in AM colonization [55].…”

Section: Phenol Extraction Of Proteinsmentioning

confidence: 99%

Proteomic studies of arbuscular mycorrhizal associations

Couto¹,

Lovato²,

Wipf³

et al. 2013

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Arbuscular mycorrhizal (AM) fungi are soil-borne microorganisms forming mutualistic associations with the vast majority of land plants, including most agricultural relevant crops. In this association the plant provides the fungus with plant photosynthates allowing it to complete its life cycle, while the fungus provides the plant with mineral nutrients, mainly phosphorus and can also help the plant to tolerate biotic and abiotic stresses. In regard to these benefits there is growing interest on the use of AM fungi to improve productivity and sustainability in agricultural systems. AM fungi and their interactions with plants have been extensively studied using proteomic techniques, but some difficulties have been faced. 1) Little is known about the AM fungal typical protein repertoire because it is currently impossible to grow AM fungi in pure axenic cultures; 2) Plant tissues often contain high amounts of interfering substances that make protein extraction for the study of AM interactions a difficult procedure; 3) Most nutrient exchanges between AM fungi and their host plants involve participation of membrane proteins, still poorly resolved in most separation techniques. Finally, 4) the formation of the arbuscule is an asynchronous process, making it difficult to distinguish which proteins are essential in the early or late stages of AM associations. In this review we present a historical summary of how these difficulties have been overcome by technological advances in proteomics and we discuss current and future trends in the study of the proteins involved in AM interactions.

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“…Changes in polypeptide patterns in germinating spores induced by the presence of root exudates were later detected by 2D gel electrophoresis (Samra et al 1996) and the identification of fungal proteins by mass spectrometry analysis was achieved for extraradical hyphae from root organ cultures (Dumas-Gaudot et al 2004). Later, fungal proteins could be also detected inside roots (Recorbet et al 2010). In order to obtain sequence information from AM fungi, spores and extraradical hyphae were used as pure fungal biological material for DNA extractions (Simon et al 1992).…”

Section: Methodologiesmentioning

confidence: 99%