Identification of immunoglobulin E binding components of two major tree pollens, birch and alder

Yoon, Moon-Gyung; Kim, Mi-Ae; Jin, Hongzhe; Shin, Yoo-Seob; Park, Hae‐Sim

doi:10.4168/aard.2013.1.3.216

Cited by 4 publications

(5 citation statements)

References 16 publications

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“…According to a previous study in Korea, the sensitization rates for Bet v 1 and 2 confirmed by an immunoblot were 78.9% and 75.8%, respectively 13. In this study, 82.0% and 19.7% of patients sensitized to birch pollen were Bet v 1- and v 2-positive, respectively.…”

Section: Discussionsupporting

confidence: 60%

“…Among these participants, 82.5% and 82.5% were sensitized to birch and oak pollens, respectively. Alder pollen sensitization (86.7%) was found to have a pattern similar to that of birch and oak, and is known to be cross-reactive to birch tree pollen 13. When sensitization was based on ImmunoCAP results, which was performed iw n 90 patients (96.8%), 98.9% and 97.8% of the patients were sensitized to both birch and oak pollen, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Alder pollen sensitization (86.7%) was found to have a pattern similar to that of birch and oak, and is known to be cross-reactive to birch tree pollen. 13 When sensitization was based on ImmunoCAP results, which was performed iw n 90 patients (96.8%), 98.9% and 97.8% of the patients were sensitized to both birch and oak pollen, respectively. The percentages of positive responses to birch component allergens were 82.0% and 19.7% for Bet v 1 and Bet v 2, respectively.…”

Section: Resultsmentioning

confidence: 99%

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Clinical Significance of Component Allergens in Fagales Pollen-Sensitized Peanut Allergy in Korea

Park

Son

Lee

et al. 2016

Allergy Asthma Immunol Res

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PurposeClinical features of peanut allergy can range from localized to systemic reactions. Because peanut and birch pollen have cross-reactivity, peanut can lead to localized allergic reaction in Fagales pollen-sensitized oral allergy syndrome (OAS) patients without peanut sensitization per se. The purpose of this study was to discriminate true peanut food allergy from cross-reactive hypersensitivity in birch-sensitized peanut allergy.MethodsBirch-sensitized (n=81) and peanut anaphylaxis patients (n=12) were enrolled. Peanut-related allergic reactions and sensitization profiles were examined. Specific IgE to Fagales tree pollens (birch, oak), peanut, and their component allergens (Bet v 1, Bet v 2, Ara h 1, Ara h 2, Ara h 3, Ara h 8, and Ara h 9) were evaluated. Based on these specific IgEs and clinical features, the patients were classified into 4 groups: group 1 (Fagales pollen allergy without OAS), group 2 (Fagales pollen allergy with OAS), group 3 (OAS with peanut anaphylaxis), and group 4 (peanut anaphylaxis).ResultsAfter peanut consumption, one-third of OAS patients experienced oral symptoms not associated with peanut sensitization. Ara h 1 or Ara h 2 was positive in peanut anaphylaxis patients, whereas Ara h 8 was positive in OAS patients. There were 4 patients with both peanut anaphylaxis and OAS (group 3). Both Ara h 2 and Ara h 8 were positive in these patients. Foods associated with OAS in Korea showed unique patterns compared to Westernized countries.ConclusionsAra h 2 and Ara h 8 may be important component allergens for discriminating peanut allergy.

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Section: Discussionsupporting

confidence: 60%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Clinical Significance of Component Allergens in Fagales Pollen-Sensitized Peanut Allergy in Korea

Park

Son

Lee

et al. 2016

Allergy Asthma Immunol Res

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“…We performed an enzyme-linked immunosorbant assay (ELISA) to detect the changes of serum sIgE to alder, birch, ragweed and mugwort pollen extracts. Pollen extracts were prepared the same as described in a previous study method ( 17 ); pollen of Alnus incana (alder), Betula pendula (birch), Ambrosia artemisiifolia (ragweed), and Artemisia vulgaris (mugwort) were purchased at Allergon® (ThermoFisher Scientific, Uppsala, Sweden). After the preparation of a crude extraction in a phosphate buffered saline (PBS), a Bradford protein assay measured the protein concentration and a sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) confirmed the protein bands.…”

mentioning

confidence: 99%

“…IgE immunoblot analysis was performed with transfer 40 µg/mL pollen extracts to polyvinylidene difluoride (PVDF membrane, Millipore Co., Bedford, MA, USA) and incubation of sera for 12 hr at 4℃. The major allergen of each pollen was defined according to previous reports; Aln g 1 (17 kDa) in alder, Bet v 1 (17 kDa) in birch, Amb a 1 (38 kDa) in ragweed and Art v 1 (24 kDa) in mugwort ( 17 , 18 ). A densitometry (Bio-Rad Universal Hood II Gel Imager, BIO-RAD Laboratories, Milan, Italy) observed the changes of IgE binding components.…”

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confidence: 99%

Paradoxical Increase of IgE Binding Components during Allergen-Specific Immunotherapy in Pollinosis Patients

et al. 2014

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Allergen-specific immunotherapy (SIT) reduces allergen specific IgE (sIgE) levels and achieves clinical and immunological tolerance by modulating innate and adaptive immunological responses. Increased temperature and CO2 concentrations caused by climate changes contribute to an increase of pollen count and allergenicity that influences clinical SIT outcomes. In this study, we investigated the changes of IgE binding components to tree and weed pollens in pollinosis patients who showed a paradoxical increase of serum sIgE level during pollen-SIT. We enrolled nine patients who showed an increasing pattern of serum sIgE level to alder, birch, ragweed and mugwort pollens by enzyme-linked immunosorbant assay. IgE immunoblot analysis confirmed the intensification or new generation of major IgE binding components that could be induced by climate change. The findings suggest that the regular monitoring of sIgE levels and symptom changes is required to improve the clinical outcomes of SIT in patients undergoing SIT for tree and weed pollens.Graphical Abstract

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Flowering Phenology and Characteristics of Pollen Aeroparticles of Quercus Species in Korea

Kim

Kwak

Lee

et al. 2020

Forests

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In recent decades, airborne allergens for allergic respiratory diseases have been found to increase significantly by a process of converting coniferous forests into broad-leaved forests in Korea. This study was conducted to evaluate factors, including airborne pollen counts, micromorphology, and flowering phenology, that can affect oak pollen-related allergic symptoms. The catkin of Mongolian oak (Quercus mongolica Fisch. ex Ledeb.) showed the most rapidly blooming catkin on Julian day 104 in flower development. Among six species, the last flowering was observed on Julian day 119 in Korean oak (Quercus dentata Thunb.). The pollen dispersal was persisted for about 32 days from Julian day 104 to Julian day 136. Airborne pollen was observed about 2 weeks after flowering phase H, the senescence phase. Pollen size varied by species, with the largest from Q. mongolica (polar axis length, PL = 31.72 µm, equatorial axis length, EL = 39.05 µm) and the smallest from Jolcham oak (Quercus serrata Murray) (PL = 26.47 µm, EL = 32.32 µm). Regarding pollen wall structure, endexine of Q. dentata was coarsely laminated or fragmented. The endexine thicknesses of Sawtooth oak (Quercus acutissima Carruth.) and Q. serrata were thick and stable, whereas Galcham oak (Quercus aliena Blume), Q. mongolica, and Oriental cork oak (Quercus variabilis Blume) had thinner endexines. The area occupied by pollenkitt of Q. variabilis was significantly larger than that of Q. acutissima. Importantly, Q. variabilis had a distinctly thick 17 kDa protein band, a presumed major allergen. Oak species differ in pollen protein composition, and thus there is a possibility that the allergenic activity of pollen proteins vary depending on oak species. This study highlights the fact that native oak species in Korea differ in flowering pattern of male flowers, pollen morphology, and pollen chemical constituents. These discrepancies in flowering and pollen properties imply variable allergic responses to oak pollen from different species.

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Identification of immunoglobulin E binding components of two major tree pollens, birch and alder

Cited by 4 publications

References 16 publications

Clinical Significance of Component Allergens in Fagales Pollen-Sensitized Peanut Allergy in Korea

Clinical Significance of Component Allergens in Fagales Pollen-Sensitized Peanut Allergy in Korea

Paradoxical Increase of IgE Binding Components during Allergen-Specific Immunotherapy in Pollinosis Patients

Flowering Phenology and Characteristics of Pollen Aeroparticles of Quercus Species in Korea

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