Identification of immunogenic consensus T-cell epitopes in globally distributed influenza-A H1N1 neuraminidase

Gupta, Shishir; Srivastava, Mugdha; Akhoon, Bashir Akhlaq; Smita, Suchi; Schmitz, Ulf; Vera, Julio; Gupta, Shailendra Kumar

doi:10.1016/j.meegid.2010.10.013

Cited by 25 publications

(12 citation statements)

References 79 publications

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“…Mutation V34I has been reported in H5N1 strains from Cambodia from 2013 where an increase in human H5N1 infections was observed [8]. No biological function has been associated with the V34I substitution, while positions 74, 244 and 284 are located in B-or T-cell antigenic regions of the NA protein [9]. For four viruses, whole genome sequences were generated and further signature mutations of the emerging viruses were found in the internal gene segments as well: Non-silent cluster-specific mutations were confined to the PB2 (M66I, T106A), PB1-F2 (G22E), and M1 (I15V) proteins (Table 3).…”

Section: Genetic Characterisationmentioning

confidence: 99%

Emergence of a novel cluster of influenza A(H5N1) virus clade 2.2.1.2 with putative human health impact in Egypt, 2014/15

Arafa¹,

Naguib

Luttermann

et al. 2015

Eurosurveillance

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Section: Genetic Characterisationmentioning

confidence: 99%

Emergence of a novel cluster of influenza A(H5N1) virus clade 2.2.1.2 with putative human health impact in Egypt, 2014/15

Arafa¹,

Naguib

Luttermann

et al. 2015

Eurosurveillance

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“…Obtained sequences were aligned, analyzed, and trimmed in BioEdit software version 7.7.9. Then, Shannon entropy values were measured to define the conserved regions since the epitopes from highly conserved regions are likely to elicit more immune responses [29,30]. This Shannon entropy analysis measures variation at each amino acid position in the set of aligned sequences.…”

Section: Methodsmentioning

confidence: 99%

Designing and Modeling of Complex DNA Vaccine Based on Tropomyosin Protein of Boophilus Genus Tick

Ranjbar

Gupta

Ghorban

et al. 2014

Appl Biochem Biotechnol

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Boophilus tick is a bloodsucking ectoparasite that transfers some pathogens, reducing production and thus leading to economical losses in the cattle industry. Tropomyosin (TPM) protein is a salivary protein, has actin regulator activity, and plays an important role in immune reactions against parasites. In the current study, besides developing a safe, effective, and broad spectrum protective measure against Boophilus genus tick based on TPM protein, we attempted to minimize possible problems occurring in the design of polytopic vaccines. Briefly, the steps that were followed in the present study were as follows: retrieving sequences and finding the mutational/conservative regions, selecting consensus and high immunogenic epitopes of B and CD4(+) T cells by different approaches, three-dimensional structure (3D structure) prediction and representation of epitopes and highly variable/conserve regions, designing vaccinal construct by fusion of B and T cell epitopes by special patterns and improving immunogenicity, evaluation of the constructs' primary structure and posttranslational modification, calculation of hydrophobic regions, reverse translation, codon optimization, open reading frame checking, insertion of start/end codon, Kozak sequence, and finally constructing the DNA vaccine. Variation plot showed some shared epitopes among the ticks' and mites' species that some might be effective only in some species. Finally, by following the steps mentioned above, two constructs for B and T cells were achieved. Checking constructs revealed their reliability and efficacy for in vitro production and utilization. Successful in silico modeling is an essential step of designing vigorous vaccines. We developed a novel protective and therapeutic vaccine against Boophilus genus (based on TPM protein). At the next step, constructed DNA vaccine would be produced in vitro and administrated to cattle, and its potency to induction of immune response and protection against Boophilus genus as well as other ticks and mites will be evaluated.

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“…and IEDB Consensus (67), were also used to predict binding of MPT83 peptides to 12 to 14 HLA-DR alleles, including HLA DRB1*0101, DRB1*0301, DRB1*0401, DRB1*0701, DRB1*1101, and DRB1*1501 supertype alleles, which are expected to cover approximately Ͼ95% of any given human population (27). The sequences/peptides predicted to bind Ն50% alleles of HLA-DR molecules analyzed were considered promiscuous for binding (45,53).…”

Section: Methodsmentioning

confidence: 99%

Comparative Evaluation of MPT83 (Rv2873) for T Helper-1 Cell Reactivity and Identification of HLA-Promiscuous Peptides in Mycobacterium bovis BCG-Vaccinated Healthy Subjects

Mustafa

2011

Clin Vaccine Immunol

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Identification of immunogenic consensus T-cell epitopes in globally distributed influenza-A H1N1 neuraminidase

Cited by 25 publications

References 79 publications

Emergence of a novel cluster of influenza A(H5N1) virus clade 2.2.1.2 with putative human health impact in Egypt, 2014/15

Emergence of a novel cluster of influenza A(H5N1) virus clade 2.2.1.2 with putative human health impact in Egypt, 2014/15

Designing and Modeling of Complex DNA Vaccine Based on Tropomyosin Protein of Boophilus Genus Tick

Comparative Evaluation of MPT83 (Rv2873) for T Helper-1 Cell Reactivity and Identification of HLA-Promiscuous Peptides in Mycobacterium bovis BCG-Vaccinated Healthy Subjects

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