Identification of IGFBP-6 as a significantly downregulated gene by β-catenin in desmoid tumors

Denys, Hannelore; Jadidizadeh, Ali; Amini-Nik, Saeid; Dam, Kim Van; Aerts, Stein; Alman, Benjamin A.; Cassiman, Jean‐Jacques; Tejpar, Sabine

doi:10.1038/sj.onc.1207160

Cited by 39 publications

(39 citation statements)

References 24 publications

(25 reference statements)

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“…In NCI-H1299 cells, TCF activity is induced by introducing b-catenin cDNA (Usami et al, 2003). The IGFBP-6 promoter has two copies of TCFbinding motifs, and b-catenin suppresses the expression of IGFBP-6 through these motifs (Denys et al, 2004). These results suggest that, in NCI-H1299 cells, b-catenin may antagonize the tumor suppressor activity of SEMA3B by inhibiting the expression of IGFBP-6.…”

Section: Identification Of Igfbp-6mentioning

confidence: 71%

Identification of IGFBP-6 as an effector of the tumor suppressor activity of SEMA3B

et al. 2008

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Section: Identification Of Igfbp-6mentioning

confidence: 71%

Identification of IGFBP-6 as an effector of the tumor suppressor activity of SEMA3B

et al. 2008

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“…Increased β-catenin levels have been causatively linked to increased broblast proliferation in aggressive bromatosis (desmoid tumor) [27,28] and hypertrophic scar formation [22]. As these conditions share many molecular similarities with FSS and DD [18,29], β-catenin is likely to play analogous broproliferative roles in these diseases. Western immunoblotting also revealed the presence of multiple molecular weight forms of β-catenin in FSS tissues, correlating with similar observations in DD tissues [17].…”

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confidence: 99%

IGF2 expression and β-catenin levels are increased in Frozen Shoulder Syndrome

Raykha¹,

Crawford²,

Burry³

et al. 2014

CIM

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IGF2 expression and β-catenin levels are increased in Frozen Shoulder Syndrome Abstract Purpose: Frozen Shoulder Syndrome is a brosis of the shoulder joint capsule that is clinically associated with Dupuytren's disease, a brosis of the palmar fascia. Little is known about any commonalities in the pathophysiology of these connective tissue broses. β-catenin, a protein that transactivates gene expression, and levels of IGF2 mRNA, encoding insulin-like growth factor-II, are elevated in Dupuytren's disease. e aim of this study was to determine if correlating changes in β-catenin levels and IGF2 expression are evident in Frozen Shoulder Syndrome.Methods: Tissue from patients with Frozen Shoulder Syndrome and rotator cu tear were obtained during shoulder arthroscopies. Total protein extracts were prepared from tissue aliquots and β-catenin immunoreactivity was assessed by Western immunoblotting. In parallel, primary broblasts were derived from these tissues and assessed for IGF2 expression by quantitative PCR.Results: β-catenin levels were signi cantly increased in Frozen Shoulder Syndrome relative to rotator cu tear when assessed by Western immunoblotting analyses. IGF2 mRNA levels were signi cantly increased in primary broblasts derived from frozen shoulder syndrome tissues relative to broblasts derived from rotator cu tissues. Conclusions:As in Dupuytren's disease, β-catenin levels and IGF2 expression are elevated in Frozen Shoulder Syndrome. ese ndings support the hypothesis that these connective tissue broses share a common pathophysiology. Materials and Methods Tissue collectionTissue sections were collected with approval of the Human Subjects Research Ethics Board (HSREB) at Western University from surgical specimens of patients undergoing shoulder arthroscopy for the treatment of either FSS or subacromial decompression for RCT. An arthroscopic punch was used to obtain tissue specimens from the rotator cu interval immediately adjacent to the antero-superior arthroscopic portal from patients with FSS and RCT. Representative samples of these tissues were removed at the time of surgery and immediately transported to the laboratory. e tissues were either snap frozen in liquid nitrogen for total protein extraction or processed for primary broblast derivation. Western immunoblottingTotal protein extracts were prepared from snap frozen tissue using modi ed RIPA bu er. Tissue lysate (25 μg) was subjected to Western blot analysis and β-catenin levels were assessed using an anti-β-catenin monoclonal antibody (clone 14, Transduction Laboratories, Lexington, KY). β-actin levels were assessed in parallel using an anti-β-actin antibody (Sigma, St Louis, MO) to normalize for variability in total protein loading. Antibody speci c bands were visualized using enhanced chemiluminescence (ECL) and Kodak XLS lm. Densitometry analysis was carried out using Scion Image so ware (Scion Corporation, Beta 4.0.2, Frederick, MD). Normalized measurements of β-catenin were plotted as the sample mean (β-catenin /actin) ratio ± standard error...

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“…14 -16 Further findings suggest that insulin-like growth factor binding protein 6 (IGFBP-6) is downregulated directly by the ␤-catenin/TCF complex in adult AF and implies a role for the IGF axis in the proliferation of AF. 30 A recent review reported a high prevalence of desmoid tumor in 126 of 880 adult patients with FAP (14.3%) who had proven AF gene mutation, 19 and an extremely high prevalence (38%) was reported for patients with Gardner syndrome. 20 In pediatric AF studies, no patient with a history of familial AF or FAP and only two patients with Gardner syndrome were reported.…”

Section: Discussionmentioning

confidence: 99%

Pediatric aggressive fibromatosis

Buitendijk

Ven

Dumans

et al. 2005

Cancer

100

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Fine‐needle aspiration (FNA) biopsy has become a standard first‐line diagnostic procedure for palpable and nonpalpable nodules of the thyroid gland. Six cytologic diagnostic categories have recently been proposed to unify the terminology that is linked to proper clinical management. We report a case of follicular neoplasm diagnosed on FNA specimen that had a very artistic appearance of the microfollicle formation on both Diff‐Quik and Papanicolaou‐stained slides. Diagn. Cytopathol. 2010;38:660–662. © 2009 Wiley‐Liss, Inc.

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Identification of IGFBP-6 as a significantly downregulated gene by β-catenin in desmoid tumors

Cited by 39 publications

References 24 publications

Identification of IGFBP-6 as an effector of the tumor suppressor activity of SEMA3B

Identification of IGFBP-6 as an effector of the tumor suppressor activity of SEMA3B

IGF2 expression and β-catenin levels are increased in Frozen Shoulder Syndrome

Pediatric aggressive fibromatosis

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