Identification of <i>Mycobacterium</i> species in FFPE granulomatous lymphadenitis tissue using REBA Myco-ID®

Munkhdelger, Jijgee; Wang, H. Y.; Choi, Yeji; Wairagu, Peninah M.; Lee, D.; Park, Soohyung; Kim, S.; Jeon, Bo Young; Lee, HyungJune; Park, K. H.

doi:10.5588/ijtld.12.0818

Cited by 4 publications

(10 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In South Korea, MDR-TB strains account for 2.7-3.9% of new TB cases and 14.0-27.2% of previously treated cases (15). Only two South Korean studies about TB lymphadenitis have been published (16,17). They reported 373 and 110 cases of TB lymphadenitis, respectively, but only one case was diagnosed as mediastinal TB lymphadenitis with subcarinal lymph node involvement.…”

Section: Discussionmentioning

confidence: 99%

Esophagomediastinal Fistula Secondary to Multidrug-resistant Tuberculous Mediastinal Lymphadenitis

Lee

et al. 2014

Intern. Med.

View full text Add to dashboard Cite

Esophagomediastinal fistula secondary to mediastinal tuberculosis (TB) lymphadenitis is a rare and unusual complication. A 32-year-old woman visited our clinic because of chest pain. Computed tomography (CT) demonstrated an esophagomediastinal fistula with subcarinal lymphadenopathy and no remarkable parenchymal lung lesions. The esophagomediastinal fistula was confirmed by esophagoscopy; however, the patient's bronchoscopy findings were unremarkable. The endobronchial ultrasound-guided lymph node aspiration did not confirm a diagnosis of TB. Finally, the patient was diagnosed via a lymph node biopsy. A drugsensitivity test revealed the presence of a multidrug-resistant pathogen. To the best of our knowledge, this is the first case of esophagomediastinal fistula secondary to multidrug-resistant (MDR-) TB mediastinal lymphadenitis.

show abstract

Section: Discussionmentioning

confidence: 99%

Esophagomediastinal Fistula Secondary to Multidrug-resistant Tuberculous Mediastinal Lymphadenitis

Lee

et al. 2014

Intern. Med.

View full text Add to dashboard Cite

show abstract

“…Two samples each of cutaneous TB and NTM infections, which had been confirmed by nested MTB/NTM PCR, were selected as positive controls, and one sample each of condyloma acuminata and epidermal cysts were selected as negative controls. REBA Myco‐ID ® (YD Diagnostics, Yongin, Korea) was performed after deparaffinization and DNA extraction from unstained sections of formalin‐fixed, paraffin‐embedded specimens of each sample and control, in accordance with previous reports …”

Section: Case Reportmentioning

confidence: 99%

“…REBA Myco-ID â (YD Diagnostics, Yongin, Korea) was performed after deparaffinization and DNA extraction from unstained sections of formalin-fixed, paraffin-embedded specimens of each sample and control, in accordance with previous reports. 10 This study was approved by the institutional review board of Wonju Severance Christian Hospital (CR318042).…”

Section: Case Reportmentioning

confidence: 99%

“…The method has shown high sensitivity and specificity for identifying mycobacterial DNA and can differentiate between 18 different mycobacterial species, including MTB and non‐tuberculous mycobacteria (NTM) . REBA has been proven useful for identifying mycobacteria from the lesions of Mycobacterium ‐infected diseases …”

Section: Introductionmentioning

confidence: 99%

“…7 REBA has been proven useful for identifying mycobacteria from the lesions of Mycobacterium-infected diseases. [8][9][10] The aim of this study was to identify mycobacteria from cutaneous sarcoidosis lesions using REBA.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Mycobacterium tuberculosis and non‐tuberculous mycobacteria from cutaneous sarcoidosis lesions by reverse blot hybridization assay

Lee

Eom

Kim

et al. 2019

The Journal of Dermatology

View full text Add to dashboard Cite

While the etiology of sarcoidosis remains uncertain, mycobacteria have been suggested as a causative infectious agent. To investigate the causal relationship between mycobacteria and sarcoidosis, we performed a reverse blot hybridization assay (REBA) to identify mycobacteria from the skin samples of nine patients with sarcoidosis. Six of the nine samples were shown to be positive for mycobacteria by REBA, including Mycobacterium tuberculosis and non‐tuberculous mycobacteria. This is the first study to identify mycobacteria from the skin samples of sarcoidosis patients using REBA, and our results could strengthen the etiologic association between mycobacteria and sarcoidosis.

show abstract

Comparison of One-Tube Nested-PCR and PCR-Reverse Blot Hybridization Assays for Discrimination of Mycobacterium tuberculosis and Nontuberculous Mycobacterial Infection in FFPE tissues

Park

Bae

et al. 2019

BSL

View full text Add to dashboard Cite

Currently, molecular diagnostic assays based on nucleic acid amplification tests have been shown to effectively detect mycobacterial infections in various types of specimen, however, variable sensitivity was shown in FFPE samples according to the kind of commercial kit used. The present study therefore used automated PCR-reverse blot hybridization assay (REBA) system, REBA Myco-ID HybREAD 480 ® , for the rapid identification of Mycobacterium species in various types of human tissue and compared the conventional one-tube nested-PCR assay for detecting Mycobacterium tuberculosis (MTB). In conventional nested-PCR tests, 25 samples (48%) were MTB positive and 27 samples (52%) were negative. In contrast, when conducted PCR-REBA assay, 11 samples (21%) were MTB positive, 20 samples (39%) were NTM positive, 8 samples (15%) were MTB-NTM double positive, and 13 samples (25%) were negative. To determine the accuracy and reliability of the two molecular diagnostic tests, the one-tube nested-PCR and PCR-REBA assays, were compared with histopathological diagnosis in discordant samples. When conducted nested-PCR assay, 10 samples (59%) were MTB positive and seven samples (41%) were negative. In contrast, when conducted PCR-REBA test, three samples (17%) were MTB positive, 10 samples (59%) were NTM positive and four samples (24%) were negative. In conclusion, the automated PCR-REBA system proved useful to identify Mycobacterium species more rapidly and with higher sensitivity and specificity than the conventional molecular assay, one-tube nested-PCR; it might therefore be the most suitable tool for identifying Mycobacterium species in various types of human tissue for precise and accurate diagnosis of mycobacterial infection.

show abstract

Identification of Mycobacterium species in FFPE granulomatous lymphadenitis tissue using REBA Myco-ID®

Abstract: REBA Myco-ID is a highly sensitive and specific assay for detecting M. tuberculosis and NTM. M. tuberculosis is the main cause of granulomatous lymphadenitis.

Cited by 4 publications

References 21 publications

Esophagomediastinal Fistula Secondary to Multidrug-resistant Tuberculous Mediastinal Lymphadenitis

Esophagomediastinal Fistula Secondary to Multidrug-resistant Tuberculous Mediastinal Lymphadenitis

Identification of Mycobacterium tuberculosis and non‐tuberculous mycobacteria from cutaneous sarcoidosis lesions by reverse blot hybridization assay

Comparison of One-Tube Nested-PCR and PCR-Reverse Blot Hybridization Assays for Discrimination of Mycobacterium tuberculosis and Nontuberculous Mycobacterial Infection in FFPE tissues

Contact Info

Product

Resources

About