Identification of CD28 and PTEN as novel prognostic markers for cervical cancer

Abstract

Cervical cancer (CC) is the most common malignant tumor with poor clinical outcome among women. Identification of novel biomarkers could be beneficial for the clinical diagnosis and treatment of CC. This study aimed to identify prognostic biomarkers for the prediction of prognostic status of CC patients, and explore the effect of the corresponding methylated genes in the occurrence and development of CC. The methylation microarray data of CC was extracted from The Cancer Genome Atlas (TCGA) dataset. The methylation genes associated with the prognostic status were identified based on the information of the relapse-free survival (RFS) of the CC patients. The prognostic gene pairs were further identified. Then, the prognostic signature was identified by the forward search algorithm based on the C-index method. The results were validated by independent dataset. Finally, the functional analysis was performed on the methylation genes. A total of 276 methylation genes and 2508 gene pairs associated with the prognostic status of the CC were identified.A signature composed of eight methylation gene pairs was obtained to predict the prognostic status of cervical patients. A series of genes that played an important role in the occurrence and development of CC were obtained by the functional enrichment analysis. To summary, a prognostic signature consisting of eight methylation gene pairs was obtained. Of note, the CD28 and PTEN gene pair were found to play important roles in the occurrence and development of CC.

DNA methylation for cervical cancer screening: a training set in China

“…Third, all cervical histological pathology results in this study were based on cervical biopsies, which may involve small but maybe essential differences compared to the final pathology results, likely leading to accurate analysis and bias. Last, we did not perform a follow-up of the prognosis of the patients, limiting the interpretation of the DNA methylation data with regard to the carcinogenesis and progression of cervical cancer, as demonstrated in other studies [58]. These limitations may be resolved in future validation trials.…”

“…Her2, labeled with CY5 channel, was used as internal control and added per well. Results showing the Ct values over 36 for Her2 were defined as detection failures.Samples with a Ct value in FAM channel (for HPV6,16,31,35,39,45,58, and 68, respectively) or HEX channel (for…”

DNA methylation for cervical cancer screening: A validation trial in China

“…Second, we did not follow up on the prognosis of the patients after major surgery, limiting the interpretation of DNA methylation results in the carcinogenesis and progression of cervical cancer. [57] Third, we excluded patients who were positive for HIV from the training and validation sets. This limitation would hamper the extrapolation of the DNA methylation assay to a large cohort, as reported previously.…”

Recent Advances on the Molecular Mechanism of Cervical Carcinogenesis Based on Systems Biology Technologies

“…This study revealed that DNA methylation regulated microRNAs in human cervical cancer [54]. Furthermore, a total of 276 methylation genes that correlated with the prognostic status of the cervical cancer were reported based on methylation microarray analysis [55]. It has been reported that 12 candidate genes were identified by DNA methylation profiling for screening markers of detection of cervical cancer [56].…”