Identification of <i>Acremonium acutatum</i> and <i>Trichothecium roseum</i> isolated from Grape with White Stain Symptom in Korea

Oh, Soh-Young; Nam, Ki-Woong; Yoon, Deok-Hoon

doi:10.5941/myco.2014.42.3.269

Cited by 24 publications

(18 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Acremonium spp. are common soil and plant saprophytes [ 24 ], but some species are known as opportunistic human and animal pathogens [ 25 ] or as plant pathogens [ 26 ]. The species of this genus are morphologically characterized by colonies with slow or moderate growth; thin hyphae; simple or poorly basitonously branched, orthotropic phialides, usually gradually tapering toward the tip; and conidia that are usually small, unicellular, and aggregated in slimy heads, chains, or both [ 24 27 ].…”

Section: Resultsmentioning

confidence: 99%

New Recorded Species in Three Genera of the Sordariomycetes in Korea

et al. 2017

View full text Add to dashboard Cite

In an ongoing survey of Korean indigenous fungi, three fungal strains belonging to the Sordariomycetes were isolated from soil samples. These strains were designated KNU16-001, KNU16-002, and KNU16-009, and identified as Ambrosiella grosmanniae, Acremonium sclerotigenum, and Trichocladium asperum, respectively, based on morphological characterization and phylogenetic analysis using internal transcribed spacer region sequences of ribosomal DNA. This is the first report of these species in Korea.

show abstract

Section: Resultsmentioning

confidence: 99%

New Recorded Species in Three Genera of the Sordariomycetes in Korea

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Most enriched GO terms of up DEGs were single-organism process (315, 20.78%), single-organism cellular process (290, 19.13%), single-organism metabolic process (219, 14.45%), and catabolic process (102, 6.73%) in the biological process category. Additionally, genes involved in oxacid metabolism (70,4.62%) and oxi-reduction process (67, 4.42%) were also up regulated. High enrichment in the subcellular components localized in the cell (342, 22.56%), cell part (341, 22.49%), intracellular (320, 21.11%), and intracellular part (315, 2.078%).…”

Section: Go Analysis Of Degsmentioning

confidence: 99%

“…Postharvest fungal diseases are responsible for quality deterioration of fruits and vegetables and for resultant severe losses of produce during handling, transportation, and storage. Phytopathogen Trichothecium roseum is one of the most significant spoilage fungi in China, causing decay in stored apples [1], melons [2,3], grapes [4], strawberries [5], and oranges [6]. Presently the pathogen can be controlled predominantly by chemical fungicides [7].…”

Section: Introductionmentioning

confidence: 99%

Cuminal Inhibits Trichothecium roseum Growth by Triggering Cell Starvation: Transcriptome and Proteome Analysis

Zhang

et al. 2020

Microorganisms

View full text Add to dashboard Cite

Trichothecium roseum is a harmful postharvest fungus causing serious damage, together with the secretion of insidious mycotoxins, on apples, melons, and other important fruits. Cuminal, a predominant component of Cuminum cyminum essential oil has proven to successfully inhibit the growth of T. roseum in vitro and in vivo. Electron microscopic observations revealed cuminal exposure impaired the fungal morphology and ultrastructure, particularly the plasmalemma. Transcriptome and proteome analysis was used to investigate the responses of T. roseum to exposure of cuminal. In total, 2825 differentially expressed transcripts (1516 up and 1309 down) and 225 differentially expressed proteins (90 up and 135 down) were determined. Overall, notable parts of these differentially expressed genes functionally belong to subcellular localities of the membrane system and cytosol, along with ribosomes, mitochondria and peroxisomes. According to the localization analysis and the biological annotation of these genes, carbohydrate and lipids metabolism, redox homeostasis, and asexual reproduction were among the most enriched gene ontology (GO) terms. Biological pathway enrichment analysis showed that lipids and amino acid degradation, ATP-binding cassette transporters, membrane reconstitution, mRNA surveillance pathway and peroxisome were elevated, whereas secondary metabolite biosynthesis, cell cycle, and glycolysis/gluconeogenesis were down regulated. Further integrated omics analysis showed that cuminal exposure first impaired the polarity of the cytoplasmic membrane and then triggered the reconstitution and dysfunction of fungal plasmalemma, resulting in handicapped nutrient procurement of the cells. Consequently, fungal cells showed starvation stress with limited carbohydrate metabolism, resulting a metabolic shift to catabolism of the cell's own components in response to the stress. Additionally, these predicaments brought about oxidative stress, which, in collaboration with the starvation, damaged certain critical organelles such as mitochondria. Such degeneration, accompanied by energy deficiency, suppressed the biosynthesis of essential proteins and inhibited fungal growth.fungicides to be used for postharvest treatment, reduced efficacy due to the emergence of pathogen resistance to these chemicals, and the growing public concerns over chemical residues in food and the environment have necessitated the seeking of alternative solutions to control the fungal spoilage and to guarantee food safety and consumer health [8].Essential oils (EOs), generally recognized as safe by the US Food and Drug Administration [9], are natural substances with established antimicrobial activities [10,11] and are a candidate instrument for controlling postharvest disease [12,13]. Nonetheless, this candidacy is compromised by the expensiveness of these natural substances due to their generally low yields. However, exploring their mode of actions is valuable to the development of novel alternatives.The multicomponent EOs attribute their mode of act...

show abstract

“…국내에서 Trichothecium roseum에 의한 병해로는 사과 분홍빛열매썩음병 (Kwon et al, 2014), 배 붉은빛썩음병 (Park, 1961), 멜론 분홍빛열매썩음병 (Kwon et al, 1998), 딸기 분홍빛열매썩음병 (Kwon et al, 2010), 배 분홍빛곰팡이병 (Kwon et al, 2013), 감귤 분홍빛열매썩음 병 (Kwon et al, 2013), 토마토 분홍빛썩음병 (Han et al, 2013) 포도흰얼룩병 (Oh et al, 2014a) 등이 보고된 바 있다.…”

Section: 서 언unclassified

Antifungal Activity of Bacillus Subtilis HK2 against Trichothecium Roseum Causing Pink Rot of Melon and White Stain Symptom on Grape

Oh¹,

Lee²,

Nam

et al. 2016

Korean Journal of Plant Resources

Self Cite

View full text Add to dashboard Cite

-Pink Rot on melon and White Stain Symptom on grape are caused by Trichothecium roseum, one of the most important diseases of grape and melon. These diseases have been occurred in national-wide in Korea and causes irreversible damage on the grape and the melon at harvest season. This research presents the evaluation of the capacity of Bacillus subtillis HK2 to protect both melon and grape against T. reseum and establishes its role as a biocontrol agent. In this study, we isolated a Bacillus strain HK2 from rhizosphere soil, identified it as Bacillus subtillis by 16S rRNA analysis and demonstrated its antifungal activity against T. roseum. Under I-plate assay it was observed that the effect of hyphal growth inhibition was not due to production of volatile compounds. The optimum culture condition of HK2 was found at 30℃ and initial pH of 7.0. Application of HK2 culture suspension reduced 90.2% of white stain symptom on grape as compared to control, resulting in greater protection to grape against T. roseum infestation. Butanol extract of HK2 culture purified using flash column chromatography. The antifungal material was a polar substance as it showed antifungal activity in polar elute. Therefore, our results indicated a clear potential of B. subtilis HK2 to be used for biocontrol of Pink rot in melon and white stain symptom on grape caused by T. roseum.

show abstract

Identification of Acremonium acutatum and Trichothecium roseum isolated from Grape with White Stain Symptom in Korea

Cited by 24 publications

References 10 publications

New Recorded Species in Three Genera of the Sordariomycetes in Korea

New Recorded Species in Three Genera of the Sordariomycetes in Korea

Cuminal Inhibits Trichothecium roseum Growth by Triggering Cell Starvation: Transcriptome and Proteome Analysis

Antifungal Activity of Bacillus Subtilis HK2 against Trichothecium Roseum Causing Pink Rot of Melon and White Stain Symptom on Grape

Contact Info

Product

Resources

About