Identification of hydrogen peroxide responsive ESTs involved in phenylethanoid glycoside biosynthesis in Cistanche salsa cell culture

Chen, J.; Yan, Yi; Guo, Zhigang

doi:10.1007/s10535-015-0541-y

Cited by 12 publications

(5 citation statements)

References 25 publications

(22 reference statements)

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“…Nanoparticles, Nano silver and Nano titanium can quickly and positively effects on expression of genes which are involved biosynthesis of sanguinarine in Papaver somniferum L. [40], as well as alkaloids in P. orientale L. [41]. Biosynthesis of aloin was enhanced by Nano silver and Nano titanium [9]; as well phenylethanoid glycosides biosynthesis was increasingly stimulated by Nano Chitosan [12]. Moreover, it has been reported in some studies that NanoFe application can be increas-ingly effective to growth, yield parameters and physiological features of soybean [14]and carrot [15].…”

Section: Resultsmentioning

confidence: 99%

“…It has also reported that nano titanium dioxide effects on Nitrogen metabolism by enhancing nitrate reductase and glutamate dehydrogenase activity to increase photosynthesis and growth [11]. Nano Chitosan is used to improve phenylethanoid glycosides biosynthesis in cell suspension culture of Cistanche deserticola Y.C.Ma [12] Iron (Fe) nanoparticles can be formed in the form of iron oxide. They have been known as low-toxic and high eco-friendly nanoparticles [13].…”

Section: Introductionmentioning

confidence: 99%

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Nanoelicitors Application Promote Antioxidant Capacity of <i>Asparagus officinalis</i> (In Vitro)

Mohammadhassan

Ferdosi

Malmir

et al. 2021

J.Trop Life Science

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Nanoparticles recently play remarkable roles in modern agriculture and biotechnology due to specific exclusively physicochemical and biological characteristics of the particles. In recent years, nanoparticles are been using as fertilizers and elicitors to improve crops. Nutritionists are constantly seeking natural antioxidants without side effects to using for healthcare and treatment. Asparagus officinalis L. as medicinal plant treated by iron (0, 10, 50 and 100 mg/L) and selenium (0, 0.5,and 1 mg/L) nanoparticles as nano elicitors. Then the antioxidant capacity of A. officinalis L. was detected and measured by Î±, Î±-diphenyl-Î²-picrylhydrazyl (DPPH) assay, for assessment of the antioxidant activity. The iron nanoparticlesconcentration significantly increases the antioxidant activity of both male and female asparagus stem, as well as selenium nanoparticles. When combined iron and selenium used as nano elicitors then cause the antioxidant activity significantly decreases. But the integration of two nano elicitors (iron and selenium) decreased antioxidant capacity while the use of nano-selenium could enhance antioxidant capacity. The application of nano elicitor increased antioxidant capacity in the female stem than male.Nanoparticles recently play remarkable roles in modern agriculture and biotechnology due to specific exclusively physicochemical and biological characteristics of the particles. In recent years, nanoparticles are been using as fertilizers and elicitors to improve crops. Nutritionists are constantly seeking natural antioxidants without side effects to using for healthcare and treatment. Asparagus officinalis L. as medicinal plant treated by iron (0, 10, 50 and 100 mg/L) and selenium (0, 0.5,and 1 mg/L) nanoparticles as nano elicitors. Then the antioxidant capacity of A. officinalis L. was detected and measured by Î±, Î±-diphenyl-Î²-picrylhydrazyl (DPPH) assay, for assessment of the antioxidant activity. The iron nanoparticlesconcentration significantly increases the antioxidant activity of both male and female asparagus stem, as well as selenium nanoparticles. When combined iron and selenium used as nano elicitors then cause the antioxidant activity significantly decreases. But the integration of two nano elicitors (iron and selenium) decreased antioxidant capacity while the use of nano-selenium could enhance antioxidant capacity. The application of nano elicitor increased antioxidant capacity in the female stem than male.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nanoelicitors Application Promote Antioxidant Capacity of <i>Asparagus officinalis</i> (In Vitro)

Mohammadhassan

Ferdosi

Malmir

et al. 2021

J.Trop Life Science

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“…Three biologically independent experiments were performed and a total of 12 samples were obtained. Total RNA was isolated from each of the samples as described before [25]. Generally, the cells were frozen in liquid nitrogen and ground to powder.…”

Section: Methodsmentioning

confidence: 99%

Regulation Mechanism of MYC Family Transcription Factors in Jasmonic Acid Signalling Pathway on Taxol Biosynthesis

Cui

Mao

Chen

et al. 2019

IJMS

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Paclitaxel is an important anticancer drug. The phytohormone jasmonic acid can significantly induce the biosynthesis of paclitaxel in Taxus, but the molecular mechanism has not yet been resolved. To establish the jasmonic acid signalling pathway of Taxus media, based on the gene of the jasmonic acid signalling pathway of Arabidopsis thaliana, sequence analysis was performed to isolate the jasmonic acid signal from the transcriptome, a transcriptional cluster of pathway gene homologs and the full length of 22 genes were obtained by RACE PCR at 5′ and 3′: two EI ubiquitin ligase genes, COI1-1 and COI1-2;7 MYC bHLH type transcription factor (MYC2, MYC3, MYC4, JAM1, JAM2, EGL3, TT8); 12 JAZ genes containing the ZIM domain; and MED25, one of the components of the transcriptional complex. The protein interaction between each were confirmed by yeast two hybridization and bimolecular fluorescence complementation based on similar genes interaction in Arabidopsis. A similar jasmonate signaling pathway was illustrated in T. media. All known paclitaxel biosynthesis genes promoters were isolated by genome walker PCR. To investigate the jasmonate signaling effect on these genes’ expression, the transcription activity of MYC2, MYC3 and MYC4 on these promoters were examined. There are 12, 10 and 11 paclitaxel biosynthesis genes promoters that could be activated by MYC2, MYC3 and MYC4.

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“…Three times biological independent experiments were performed and a total of 12 samples were obtained. Total RNA was isolated from each of the sample as described before (Chen et al 2015). Generally, the cells were frozen in liquid nitrogen and ground to powder.…”

Section: Methodsmentioning

confidence: 99%

Identification of early jasmonate-responsive genes in Taxus × media cells by analyzing time series digital gene expression data

Mao

Chen

et al. 2018

Physiol Mol Biol Plants

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Jasmonate, an effective elicitor, can induce the biosynthesis of paclitaxel, a well-known anticancer drug, in cell culture. The jasmonate signaling pathway has been well studied in Arabidopsis, and many early jasmonate-responsive genes have been found to be involved in signaling pathway. In, only a few late jasmonate-responsive genes related to paclitaxel biosynthesis were identified. So, identification of early responsive genes and knowledge of the jasmonate signaling pathway are essential for understanding the effects of jasmonate on paclitaxel biosynthesis and for improving paclitaxel production in cells. In this study, total RNA of × cells cultured in liquid medium was extracted after 0, 0.5, 3, and 24 h of methyl jasmonate treatment. Three biological independent repetitions were performed. The 12 extracted RNA samples were integrated and sequenced on an Illumina HiSeq 2500 platform using the paired-end method. A total of 45,583 transcript clusters were obtained by de novo assembly of the sequenced reads. Based on the transcriptome data, the digital gene expressions of each RNA sample were investigated. We found that after 0.5, 3, and 24 h of methyl jasmonate treatment; 134, 1008, and 987 unigenes were differentially expressed. For the secondary metabolism pathways, phenylalanine pathway unigenes were responsive to jasmonate after 3 h of treatment, while genes related to paclitaxel biosynthesis were induced after 0.5 h of treatment. The digital gene expression levels of candidate genes related to paclitaxel biosynthesis were confirmed by qRT-PCR. Transcriptome sequencing and digital gene expression profiling identified early jasmonate-responsive genes in cultured × cells. The comprehensive time series jasmonate-responsive gene expression data have provided transcriptome-wide information about the mechanism of paclitaxel biosynthesis regulation by jasmonate signaling.

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Identification of hydrogen peroxide responsive ESTs involved in phenylethanoid glycoside biosynthesis in Cistanche salsa cell culture

Cited by 12 publications

References 25 publications

Nanoelicitors Application Promote Antioxidant Capacity of <i>Asparagus officinalis</i> (In Vitro)

Nanoelicitors Application Promote Antioxidant Capacity of <i>Asparagus officinalis</i> (In Vitro)

Regulation Mechanism of MYC Family Transcription Factors in Jasmonic Acid Signalling Pathway on Taxol Biosynthesis

Identification of early jasmonate-responsive genes in Taxus × media cells by analyzing time series digital gene expression data

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