Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism

Abstract

ABSTRACT:The aim of the study was to identify heterosomes in the semen of three Romanov rams -carriers of leukocyte chimerism (FISH technique) and to determine the proportions between spermatozoa with X and Y chromosomes. The choice of bovine probes for hybridization with ram heterosomes was dictated by genetic conservatism of bovine and ovine heterosomes. The ratio between spermatozoa with a yellow fluorescent signal containing the X chromosome in the haploid set and spermatozoa with a red-purple signal indicating the presence of the Y chromosome, taking into account spermatozoa with no signal, was 52%:43%:5% in ram No. PL100006077676; 47%:44%:9% in ram No. PL100006078031; and 48%:46%:6% in ram No. PL100006078895. The results obtained lead us to conclude that the 54,XX/54,XY chimerism has no effect on sex ratio in offspring.

Breeding and Economic Aspects of Cytogenetic Screening Studies of Pigs Qualified for Reproduction

“…Apart from balanced chromosome mutations, pigs are sporadically diagnosed with sex chromosome aneuploidies (mainly in a mosaic form) [13,[50][51][52][53], and phenotypically normal boars or individuals with hermaphroditic characteristics are increasingly diagnosed with leukocytic chimerism (XX/XY) [1,3,13,19,22,[54][55][56][57][58].…”

“…Conventional cytogenetic diagnostics are based on the microscopic analysis of metaphase chromosomes obtained from in vitro lymphocyte culture that is nondifferentially stained with Giemsa, which allows the determination of the diploid number (2n = 38) and morphology of chromosomes (submetacentric, metacentric, subtelocentric, and telocentric) ( Figure 1). individuals with hermaphroditic characteristics are increasingly diagnosed with leukocytic chimerism (XX/XY) [1,3,13,19,22,[54][55][56][57][58].…”

Legitimacy of systematic karyotype evaluation of cattle qualified for reproduction

“…Z kolei wpływ obecności osobników żeńskich w okresie rozwoju zarodkowego na późniejszą przydatność rozpłodową samców pochodzących z bliźniąt lub wieloraczków różnej płci jest przedmiotem wielu dyskusji i analiz (14,16). Liczne badania bliźniąt bydlęcych wykazały, że na ogół buhaje z chimeryzmem leukocytarnym 60,XX/60,XY charakteryzują się parametrami płodności, jakości nasienia i jego przydatności do mrożenia, nie odbiegającymi od ogól-nie przyjętych norm i w związku z tym takie buhajki można warunkowo dopuścić do rozrodu.…”