Legitimacy of systematic karyotype evaluation of cattle qualified for reproduction

Kozubska‐Sobocińska, A.; Danielak-Czech, B.

doi:10.21521/mw.5763

Cited by 3 publications

(4 citation statements)

References 22 publications

(40 reference statements)

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“…Most genetic testing performed in breeding programmes has stressed genomic evaluation of bulls (Jennings et al, 2020), while relatively little attention is given to cytogenetic testing. Most often, cytogenetic screening tests are performed to identify chromosomal aberrations in young bulls before they are determined to be qualified for reproduction (Kozubska‐Sobocińska & Danielak‐Czech, 2017). Karyotype monitoring, including identification of chromosomal instabilities, enables culling of animals with chromosomal abnormalities, as well as assessment of the fertility of even young animals, as these instabilities can have harmful effects on future pregnancies (Jennings et al, 2020; Selvan et al, 2021; Tutt et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Assessment of the genomic stability of calves obtained from artificial insemination and OPU/IVP in vitro fertilization

Kępka

Wójcik

Wysokińska

2023

Reprod Domestic Animals

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Damage to genetic material and errors in the functioning of cellular mechanisms disturb genome stability and integrity. Assessment of genomic stability in animals is a very important aspect of breeding work. Unfortunately, harmful instabilities affect the functioning, health and reproductive processes of animals. Obtaining healthy calves is a priority, whatever methods of reproductive biotechnology are applied. The aim of the study was to assess the genomic stability of calves obtained from artificial insemination and OPU/IVP in vitro fertilization. The genomic stability of the calves was evaluated using the comet, sister chromatid exchange, and fragile sites assays. Damage to the genetic material of calves obtained by two reproductive biotechnologies was identified. Identification of instability in animals can be a valuable tool in breeding work and accelerate breeding progress.

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Section: Discussionmentioning

confidence: 99%

Assessment of the genomic stability of calves obtained from artificial insemination and OPU/IVP in vitro fertilization

Kępka

Wójcik

Wysokińska

2023

Reprod Domestic Animals

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“…Silver foxes had a more stable genome than blue foxes and hybrids. Vaneste et al [41], Alfarawati et al [42], Kozubska-Sobicińska, and Danielak-Czech [43] attribute reproductive failure to Robertsonian translocation, among other factors. Although the animals have a normal phenotype, they are affected by impaired fertility, oestrous cycle disturbances, and an extended inter-birth interval.…”

Section: Discussionmentioning

confidence: 99%

Chromosomal Instability at Fragile Sites in Blue Foxes, Silver Foxes, and Their Interspecific Hybrids

Kuchta-Gładysz

Wójcik

Grzesiakowska

et al. 2021

Animals

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A cytogenetic assay based on fragile sites (FS) enables the identification of breaks, chromatid gaps, and deletions. In healthy individuals, the number of these instabilities remains low. Genome stability in these species is affected by Robertsonian translocations in the karyotype of the blue fox and by B chromosomes in the silver fox. The aims of the study were to characterise the karyotype of blue foxes, silver foxes, and their hybrids and to identify chromosomal fragile sites used to evaluate genome stability. The diploid number of A chromosomes in blue foxes ranged from 48 to 50, while the number of B chromosomes in silver foxes varied from one to four, with a constant number of A chromosomes (2n = 34). In interspecific hybrids, both types of karyotypic variation were identified, with the diploid number of A chromosomes ranging from 40 to 44 and the number of B chromosomes varying from 0 to 3. The mean frequency of FS in foxes was 4.06 ± 0.19: 4.61 ± 0.37 in blue foxes, 3.46 ± 0.28 in silver foxes, and 4.12 ± 0.22 in hybrids. A relationship was identified between an increased number of A chromosomes in the karyotype of the hybrids and the frequency of chromosomal breaks. The FS assay was used as a biomarker for the evaluation of genomic stability in the animals in the study.

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“…Preliminary research has shown that this method is highly useful for diagnosing submicroscopic chromosomal rearrangements (impossible to detect with common cytogenetic tools), which may increase the detectability of reciprocal translocations, of which the frequency in the populations is probably much higher than previously estimated. For this reason, it seems especially justifiable to use this research tool prophylactically to assess the karyotypes of elite boars in AI centers (Table 1) [7,14,31,94].…”

Section: Cytomolecular Diagnosticsmentioning

confidence: 99%

“…The highest effectiveness of these activities will be ensured by the early prevention of cytogenetic defects based on the general principle of qualifying those young animals for breeding herds, which are screened for karyotype normality before their reproductive use [4,12]. In some countries, identification of chromosomal aberration carriers and their elimination from breeding is an established standard or a legal obligation arising from the implementation of tasks related to the organization of farm animal breeding and reproduction and from sire evaluation and selection programs to ensure planned breeding progress [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Breeding and Economic Aspects of Cytogenetic Screening Studies of Pigs Qualified for Reproduction

Danielak-Czech

Kozubska‐Sobocińska

Smołucha

et al. 2020

Animals

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Cytogenetic monitoring allows the identification and early removal of pigs affected by inherited karyotype defects from breeding herds. These abnormalities cause developmental anomalies, considerably reducing the fertility (by several dozen to 100%) and performance parameters of breeding herds, resulting in substantial financial losses. This mainly concerns reciprocal translocations, typical of pigs, which are highly prevalent (about 0.46%), generally occur de novo, and normally result in low breeding soundness of the carriers. Due to the potential spontaneous occurrence of chromosomal aberrations and the rapid spread of these genetic defects in the population, especially under artificial insemination conditions, it is necessary to perform routine karyotype screening of animals qualified for reproduction. The cytogenetic screening program for young boars, carried out using continually refined diagnostic techniques, permits a precise and reliable karyotype assessment, identification of chromosomal abnormalities, and formulation of specific selection guidelines.

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Legitimacy of systematic karyotype evaluation of cattle qualified for reproduction

Cited by 3 publications

References 22 publications

Assessment of the genomic stability of calves obtained from artificial insemination and OPU/IVP in vitro fertilization

Assessment of the genomic stability of calves obtained from artificial insemination and OPU/IVP in vitro fertilization

Chromosomal Instability at Fragile Sites in Blue Foxes, Silver Foxes, and Their Interspecific Hybrids

Breeding and Economic Aspects of Cytogenetic Screening Studies of Pigs Qualified for Reproduction

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