Identification of hepatitis A virus mimotopes by phage display, antigenicity and immunogenicity

Larralde, Osmany; Martínez, Ricardo Montero; Camacho, Frank; Amin, Nevis; Aguilar, Alicia; Talavera, Arturo; Stott, David I.; Pérez, Eduardo

doi:10.1016/j.jviromet.2006.10.015

Cited by 46 publications

(30 citation statements)

References 44 publications

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“…Thus, it has been successfully applied to identify mimotopes used to diagnose malaria (22) (23) , toxoplasmosis (24) (25) , hepatitis A (26) , neurocysticercosis (27) , strongyloidiasis (28) , thyroid cancer (29) , Chagas' disease (30) , and bovine anaplasmosis (31) , and to develop vaccine candidates against cysticercosis (32) , herpes simplex virus infection (33) , cancer (34) , taeniasis (35) , hepatitis B (36) , trichinellosis (37) , Alzheimer's disease (38) , and bovine anaplasmosis (39) . Phage display technology has been also instrumental in the selection of therapeutic agents to treat various cancers such as glioblastoma, melanoma, leukemia, and prostate and thyroid cancers (40) (41) .…”

Section: Bacteriophages As Tools For the Development Of New Vaccinesmentioning

confidence: 99%

Theranostic applications of phage display to control leishmaniasis: selection of biomarkers for serodiagnostics, vaccination, and immunotherapy

Coelho

Chávez-Fumagalli

Costa

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Phage display is a high-throughput subtractive proteomic technology used for the generation and screening of large peptide and antibody libraries. It is based on the selection of phage-fused surface-exposed peptides that recognize specifi c ligands and demonstrate desired functionality for diagnostic and therapeutic purposes. Phage display has provided unmatched tools for controlling viral, bacterial, fungal, and parasitic infections, and allowed identifi cation of new therapeutic targets to treat cancer, metabolic diseases, and other chronic conditions. This review presents recent advancements in serodiagnostics and prevention of leishmaniasis -an important tropical parasitic disease-achieved using phage display for the identifi cation of novel antigens with improved sensitivity and specifi city. Our focus is on theranostics of visceral leishmaniasis with the aim to develop biomarker candidates exhibiting both diagnostic and therapeutic potential to fi ght this important, yet neglected, tropical disease.

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Section: Bacteriophages As Tools For the Development Of New Vaccinesmentioning

confidence: 99%

Theranostic applications of phage display to control leishmaniasis: selection of biomarkers for serodiagnostics, vaccination, and immunotherapy

Coelho

Chávez-Fumagalli

Costa

et al. 2015

Rev. Soc. Bras. Med. Trop.

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“…A positive control peptide (E, mimetic of envelope dengue protein), was derived from screening a phage-displayed peptide library with the H3/6 monoclonal antibody [14]; a negative control peptide, MAP46-56, containing two HAV mimotopes (46 and 56) was previously described by Larralde et al [15].…”

Section: Synthetic Peptidesmentioning

confidence: 99%

“…(wild type control) were amplified in liquid cultures of E. coli TG1 and purified by precipitation with polyethylene glycol (PEG8000)/NaCL. Each purified phage clone was tittered, and its concentration was adjusted to 10 12 UFC/mL for ELISA assays [15].…”

Section: Polyclonalmentioning

confidence: 99%

Immunogenicity of NS4b Dengue 3 Virus Mimotope Presented to the Immune System as Multiple Antigen Peptide System

et al. 2013

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The availability of random peptide libraries displayed on bacteriophage (RPL) has provided a powerful tool for selecting sequences that mimic binding properties of natural antigen epitopes (mimotopes). These mimotopes can be used for vaccine design, drug development, and diagnostic assays. Several mimotopes have been shown to induce production of antibodies against the natural antigen. We have previously identified four dengue virus mimotopes from a phage-displayed peptide library using antidengue 3 human sera. Three of them showed similarity in their amino acid sequences with the NS4b proteins of dengue. Few studies have examined the role of NS4b proteins in the antibody response to dengue virus infection. A multiple antigen peptide (MAP) system was chemically synthesized containing this mimotope (NS4b MAP), and BALB/c mice were immunized to evaluate its immunogenicity. Antipeptide responses were induced and recognised DENV-3 infected cells as determined by immunofluorescence. The high levels of the IgG2a subtype against NS4bMAP suggest the induction of a Th1-like response. Our findings suggest that the NS4b mimotope might be a useful tool for the development of multiepitope diagnostic assays, dengue virus vaccine design, and pathogenesis studies.

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“…Several studies has shown that phage display can be an important tool for epitope mapping, the development of vaccines, therapeutic drugs, diagnostic reagents, as well as in proteomic studies [31][32][33][34][35]. This technique is very useful to study viruses which do not replicate efficiently in tissue culture like HEV [36].…”

Section: Introductionmentioning

confidence: 99%

Phage-displayed peptides that mimic epitopes of hepatitis E virus capsid

Larralde

Petrík

2017

Med Microbiol Immunol

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Hepatitis E is an emerging zoonotic infection of increasing public health threat for the UK, especially for immunosuppressed individuals. A human recombinant vaccine has been licensed only in China and is not clear whether it protects against hepatitis E virus (HEV) genotype 3, the most prevalent in Europe. The aim of this study was to use phage display technology as a tool to identify peptides that mimic epitopes of HEV capsid (mimotopes). We identified putative linear and conformational mimotopes using sera from Scottish blood donors that have the immunological imprint of past HEV infection. Four mimotopes did not have homology with the primary sequence of HEV ORF2 capsid but competed effectively with a commercial HEV antigen for binding to anti-HEV reference serum. When the reactivity profile of each mimotope was compared with Wantai HEV-IgG ELISA, the most sensitive HEV immunoassay, mimotopes showed 95.2-100% sensitivity while the specificity ranged from 81.5 to 95.8%. PepSurf algorithm was used to map affinity-selected peptides onto the ORF2 crystal structure of HEV genotype 3, which predicted that these four mimototopes are clustered in the P domain of ORF2 capsid, near conformational epitopes of anti-HEV neutralising monoclonal antibodies. These HEV mimotopes may have potential applications in the design of structural vaccines and the development of new diagnostic tests.

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Identification of hepatitis A virus mimotopes by phage display, antigenicity and immunogenicity

Cited by 46 publications

References 44 publications

Theranostic applications of phage display to control leishmaniasis: selection of biomarkers for serodiagnostics, vaccination, and immunotherapy

Theranostic applications of phage display to control leishmaniasis: selection of biomarkers for serodiagnostics, vaccination, and immunotherapy

Immunogenicity of NS4b Dengue 3 Virus Mimotope Presented to the Immune System as Multiple Antigen Peptide System

Phage-displayed peptides that mimic epitopes of hepatitis E virus capsid

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