Identification of hemopexin in tear film

Pong, Jeffrey Chiu Fai; Chu, Ching-Chi; Chu, Kai-Cheong; Poon, Terence C.W.; Ngai, Sai Ming; Pang, Chi Pui; Wang, Chi Chiu

doi:10.1016/j.ab.2010.04.036

Cited by 14 publications

(11 citation statements)

References 16 publications

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“…Hemopexin was already found increased in tears of VKC patients using a 2D‐PAGE analysis and in the cytoplasm of conjunctival epithelial cells and macrophages in impression cytology samples from VKC patients . It is a type II acute‐phase reactant glycoprotein up‐regulated by IL‐6, playing a well‐established biological role in sequestering heme.…”

Section: Discussionmentioning

confidence: 99%

Identification of human tear fluid biomarkers in vernal keratoconjunctivitis using iTRAQ quantitative proteomics

Leonardi

Palmigiano

Mazzola

et al. 2013

Allergy

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Section: Discussionmentioning

confidence: 99%

Identification of human tear fluid biomarkers in vernal keratoconjunctivitis using iTRAQ quantitative proteomics

Leonardi

Palmigiano

Mazzola

et al. 2013

Allergy

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“…Increased tear levels of IgE, tryptase, histamine, and eosinophil cationic protein (ECP) have been considered biomarkers of OA . Proteomic analysis of tear fluid has proven to be a promising approach to gain more information about the pathogenesis of diseases and lead to new diagnostic possibilities …”

Section: Ocular Sampling and Tear Biomarkersmentioning

confidence: 99%

Diagnostic tools in ocular allergy

Leonardi

Doan

Fauquert

et al. 2017

Allergy

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Ocular allergy (OA) includes a group of common and less frequent hypersensitivity disorders frequently misdiagnosed and not properly managed. The diagnosis of OA is usually based on clinical history and signs and symptoms, with the support of in vivo and in vitro tests when identification of the specific allergen is required. To date, no specific test is available for the diagnosis of the whole spectrum of the different forms of OA. The lack of recommendations on diagnosis of OA is considered a medical need not only for allergists but also for ophthalmologists. This position paper aims to provide a comprehensive overview of the currently available tools for diagnosing OA to promote a common nomenclature and procedures to be used by different specialists. Questionnaires, sign and symptom grading scales, tests, and potential biomarkers for OA are reviewed. We also identified several unmet needs in the diagnostic tools to generate interest, increase understanding, and inspire further investigations. Tools, recommendations, and algorithms for the diagnosis of OA are proposed for use by both allergists and ophthalmologists. Several unmet needs in the diagnostic tools should be further improved by specific clinical research in OA. to the information provided on the title and abstract. Each topic was reviewed by two independent experts and finally included and analyzed by the whole panel. Evidence to support each point was reviewed, and a consensus decision was made for each chapter. As evidence regarding a diagnostic procedure was limited, some of the recommendations were based on consensus-driven proposals by the task force working group.

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“…9 The key causative and upstream molecules involved in the pathologic mechanisms of VKC are yet to be characterized. In a previous study, 10 hemopexin was identified in the tear proteome of patients with VKC. In this study, we further quantified and correlated tear hemopexin concentrations in VKC with clinical severity and attempted to characterize its causative role in the development of VKC in animal models.…”

mentioning

confidence: 95%

“…Tearing is one of the most prominent symptoms. Various studies [5][6][7][8][9][10] have demonstrated an abnormal tear film in patients with VKC. During allergic inflammation, conjunctival fibroblasts can release eotaxin into the tear film that has a chemotactic effect on eosinophil infiltration in conjunctival epithelium and tears.…”

mentioning

confidence: 99%

Association of Hemopexin in Tear Film and Conjunctival Macrophages With Vernal Keratoconjunctivitis

Pong

Chu²,

Li³

et al. 2011

Arch Ophthalmol

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Vernal keratoconjunctivitis (VKC) is a chronic allergic inflammatory disease with unclear etiology and pathogenesis. We investigated the tear film proteome of patients with VKC to understand the pathologic characteristics of VKC. Methods: Tear samples were collected from healthy volunteers and patients with VKC. Electrophoresis was performed to display the tear proteomic profiles according to VKC severity. The identities of differentially expressed proteins were analyzed by mass spectrometry and quantified by enzyme-linked immunosorbent assay. Impression cytology was performed on VKC conjunctival samples to demonstrate the cellular protein expression. Allergic sensitization was performed in mice to study the pathologic role of these proteins in VKC. Results: Hemopexin, an inflammatory protein, was elevated in the tear film of patients with VKC. The increased hemopexin concentration in VKC tears was significantly associated with disease severity. Impression cytology showed specific high hemopexin expression in dekeratinized conjunctival epithelium and necrotic macrophages in patients with VKC. Immunohistochemical examination of normal lacrimal tissues from mice showed that hemopexin was not expressed in any lacrimal apparatus. Under systemic and topical sensitization and challenge using hemopexin in mice, the affected eye had mild to moderate bead discharge, chemosis, and edema with excessive macrophage infiltration and conjunctival necrosis. Conclusion: An association exists between tear hemopexin and the development and pathologic effects of VKC. Clinical Relevance: Increased hemopexin may have a role in the development of VKC.

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Identification of hemopexin in tear film

Cited by 14 publications

References 16 publications

Identification of human tear fluid biomarkers in vernal keratoconjunctivitis using iTRAQ quantitative proteomics

Identification of human tear fluid biomarkers in vernal keratoconjunctivitis using iTRAQ quantitative proteomics

Diagnostic tools in ocular allergy

Association of Hemopexin in Tear Film and Conjunctival Macrophages With Vernal Keratoconjunctivitis

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