Identification of human tear fluid biomarkers in vernal keratoconjunctivitis using iTRAQ quantitative proteomics

Leonardi, Andrea; Palmigiano, Angelo; Mazzola, E. A.; Messina, Angela; Milazzo, E. M. S.; Bortolotti, Massimo; Garozzo, Domenico

doi:10.1111/all.12331

Cited by 41 publications

(30 citation statements)

References 32 publications

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“…For the tear fluid proteome, up to around 1500 proteins have been identified in deep proteomic studies 7. Leonardi et al5 found 78 different proteins in vernal keratoconjunctivitis by iTRAQ quantitative proteomics which is a similar number of proteins found in the present study. Souza et al4 identified a large number of protease and protease inhibitors in tear fluid of one healthy subjects which was similar to findings we made in nasal mucus proteome.…”

Section: Figuresupporting

confidence: 81%

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Comparison of tear proteome in allergic rhinoconjunctivitis patients and controls with respect to pollen season

Tomazic

Liesinger

Pucher

et al. 2018

Allergy

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Section: Figuresupporting

confidence: 81%

“…Tear composition reflects the physiological condition of underlying tissue but can be altered in disease related to the eye or other organs and systemic diseases such as diabetes 2. Several tear proteomic studies for diseases other than allergic rhinoconjunctivitis exist proofing the feasibility of proteomic approaches to tear fluid 3, 4, 5, 6…”

Section: Figurementioning

confidence: 99%

Comparison of tear proteome in allergic rhinoconjunctivitis patients and controls with respect to pollen season

Tomazic

Liesinger

Pucher

et al. 2018

Allergy

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“…Chemical labeling with iTRAQ or TMT makes possible the labeling of up to 8 or 10 different samples with an isobaric label [38][39][40][41][42][43]. The label is constructed in that way that the m/z values of the peptides originating from different samples (precursor ions) are the same but during fragmentation the labeling group is released and the rest of the peptide can be used for MS/ MS based protein identification.…”

Section: Proteomic Methods Applied For Dr Diagnosismentioning

confidence: 99%

Diabetic retinopathy: Proteomic approaches to help the differential diagnosis and to understand the underlying molecular mechanisms

Csősz

Deák

Kalló

et al. 2017

Journal of Proteomics

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a b s t r a c t a r t i c l e i n f oDiabetic retinopathy is the most common diabetic eye disease and a leading cause of blindness among patients with diabetes. The appearance and the severity of the symptoms correlate with the duration of diabetes and poor blood glucose level management. Diabetic retinopathy is also categorized as a chronic low-level inflammatory disease; the high blood glucose level promotes the accumulation of the advanced glycation end products and leads to the stimulation of monocytes and macrophages. Examination of protein level alterations in tears using state-of the art proteomics techniques have identified several proteins as possible biomarkers for the different stages of the diabetic retinopathy. Some of the differentially expressed tear proteins have a role in the barrier function of tears linking the diabetic retinopathy with another eye complication of diabetes, namely the diabetic keratopathy resulting in impaired wound healing. Understanding the molecular events leading to the eye complications caused by hyperglycemia may help the identification of novel biomarkers as well as therapeutic targets in order to improve quality of life of diabetic patients. Biological significance: Diabetic retinopathy (DR), the leading cause of blindness among diabetic patients can develop without any serious symptoms therefore the early detection is crucial. Because of the increasing prevalence there is a high need for improved screening methods able to diagnose DR as soon as possible. The non-invasive collection and the relatively high protein concentration make the tear fluid a good source for biomarker discovery helping the early diagnosis. In this work we have reviewed the administration of advanced proteomics techniques used in tear biomarker studies and the identified biomarkers with potential to improve the already existing screening methods for DR detection.

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“…Open to the outside environment, the eye is widely exposed to airborne allergens. In certain cases, investigations for ocular allergy require in situ collection of samples or in vivo challenge of the ocular surface by instilling allergens . Isolated from the body, the eye may react independently to allergens.…”

Section: Introductionmentioning

confidence: 99%

“…In VKC, which is considered as an etiopathogenic model of allergic conjunctivitis, conjunctival scrapings found massive infiltration of T cells and especially eosinophils. Multiple cytokines are released in particular TH2 cells induced cytokines . Specific IgE‐mediated hypersensitivity was evidenced in at least 50% of cases .…”

Section: Introductionmentioning

confidence: 99%

Diagnosing and managing allergic conjunctivitis in childhood: The allergist’s perspective

Fauquert

2019

Pediatric Allergy Immunology

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Allergic conjunctivitis in childhood often poses problems of diagnosis and management for the allergist. We present the salient points concerning the diagnosis and treatment of ocular allergy emerging from a large cohort survey conducted jointly in the departments of ophthalmology and paediatric allergy in a French teaching hospital. Seasonal acute conjunctivitis is a common disorder and not overly difficult to diagnose and treat when associated with rhinitis leading to allergic rhinoconjunctivitis. An ophthalmologist should be consulted when conjunctivitis occurs alone and if another form of conjunctivitis is suspected, such as perennial allergic conjunctivitis, vernal keratoconjunctivitis or atopic keratoconjunctivitis. When IgE‐mediated hypersensitivity assessment does not establish aetiological diagnosis, a conjunctival allergen provocation test can be performed. The principal non‐IgE‐mediated allergy is chronic blepharoconjunctivitis. The main problem for differential diagnosis is the presence of signs suggestive of dry eye. Management includes non‐pharmacological treatments, such as lacrimal substitutes, avoidance measures and protection of the ocular surface. Second‐line treatment consists of eye drops, preferably single dose or without additives and with dual local action, mast cell stabilizer action and antihistaminic action. Third‐line treatment is reserved for severe forms. Short‐lasting local steroid therapy can control flare‐ups of allergic keratoconjunctivitis, which should have specialized follow‐up. Cyclosporine is a disease‐modifying treatment, which is both effective and well tolerated.

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Identification of human tear fluid biomarkers in vernal keratoconjunctivitis using iTRAQ quantitative proteomics

Cited by 41 publications

References 32 publications

Comparison of tear proteome in allergic rhinoconjunctivitis patients and controls with respect to pollen season

Comparison of tear proteome in allergic rhinoconjunctivitis patients and controls with respect to pollen season

Diabetic retinopathy: Proteomic approaches to help the differential diagnosis and to understand the underlying molecular mechanisms

Diagnosing and managing allergic conjunctivitis in childhood: The allergist’s perspective

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