Identification of Helicobacter spp. in bile and gallbladder tissue of patients with symptomatic gallbladder disease

Sabbaghian, M. Shirin; Ranaudo, Jeffrey; Zeng, Lin; Alongi, Alexandra P.; Perez-Perez, Guillermo I.; Shamamian, Peter

doi:10.1111/j.1477-2574.2009.00148.x

Cited by 12 publications

(7 citation statements)

References 24 publications

(44 reference statements)

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“…Other Helicobacter species have also been isolated from patients with biliary diseases like cholelithiasis, cholecystitis, gall bladder polyp, and gall bladder and biliary tract cancers ( 64 – 66 ).…”

Section: Alteration Of the Microbiome-bile Salt Homeostasis In Hepatimentioning

confidence: 99%

Interactions between Bacteria and Bile Salts in the Gastrointestinal and Hepatobiliary Tracts

2017

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Bile salts and bacteria have intricate relationships. The composition of the intestinal pool of bile salts is shaped by bacterial metabolism. In turn, bile salts play a role in intestinal homeostasis by controlling the size and the composition of the intestinal microbiota. As a consequence, alteration of the microbiome–bile salt homeostasis can play a role in hepatic and gastrointestinal pathological conditions. Intestinal bacteria use bile salts as environmental signals and in certain cases as nutrients and electron acceptors. However, bile salts are antibacterial compounds that disrupt bacterial membranes, denature proteins, chelate iron and calcium, cause oxidative damage to DNA, and control the expression of eukaryotic genes involved in host defense and immunity. Bacterial species adapted to the mammalian gut are able to endure the antibacterial activities of bile salts by multiple physiological adjustments that include remodeling of the cell envelope and activation of efflux systems and stress responses. Resistance to bile salts permits that certain bile-resistant pathogens can colonize the hepatobiliary tract, and an outstanding example is the chronic infection of the gall bladder by Salmonella enterica. A better understanding of the interactions between bacteria and bile salts may inspire novel therapeutic strategies for gastrointestinal and hepatobiliary diseases that involve microbiome alteration, as well as novel schemes against bacterial infections.

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Section: Alteration Of the Microbiome-bile Salt Homeostasis In Hepatimentioning

confidence: 99%

Interactions between Bacteria and Bile Salts in the Gastrointestinal and Hepatobiliary Tracts

2017

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“…can be found in various animal species, including horses, pigs, cattle, and birds [ 2 , 3 , 5 , 6 ]. These bacteria can colonize the liver, bile, gall bladder, and lining of the gastrointestinal tract [ 9 25 ] and can cause damage or reside in the host without causing any clinical signs. To date, 35 formally named Helicobacter species have been identified.…”

Section: Introductionmentioning

confidence: 99%

Helicobacter apodemussp. nov., a newHelicobacterspecies identified from the gastrointestinal tract of striped field mice in Korea

et al. 2015

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A novel Helicobacter species was identified from the gastrointestinal tract of the Korean striped field mouse (Apodemus agrarius). Biochemical testing, ultrastructure characterization, and 16S rRNA gene sequence analysis suggested that this bacterium represents a distinct taxon. The bacterium was positive for urease activity, susceptible to cephalothin and nalidixic acid, and weakly positive for oxidase and catalase activity. Electron microscopy revealed that the bacterium has spirally curved rod morphology with singular bipolar nonsheathed flagella. Genotypically, the isolated bacterial strains (YMRC 000215, YMRC 000216, and YMRC 000419) were most closely related to a reference strain of Helicobacter mesocricetorum (97.25%, 97.32%, and 97.03% 16S rRNA sequence similarities, respectively). The 16S rRNA sequences of these strains were deposited into GenBank under accession numbers AF284754, AY009129, and AY009130, respectively. We propose the name Helicobacter apodemus for this novel species.

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“…6 Recent work demonstrated the presence of Helicobacter pylori in the bile, contributing to cholesterol gall stones. [7][8][9] In a study, Monstein show that 27.3% cases of gall stone are positive for Helicobacter pylori and helicobacter spp was found in 32% gallstones by PCR amplification. 10 A study conducted at Aga Khan University Hospital showed detection of Helicobacter pylori in gallbladder specimen of 24% of patient with gall stones disease.…”

Section: Introductionmentioning

confidence: 99%

Helicobacter Pylori

Hussain¹,

Shar²,

Sangri³

2018

TPMJ

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Objectives: To determine the frequency of helicobacter pylori in gallbladder in patient with gall stones disease. Study Design: Cross-sectional study. Place and Duration of Study: This study was conducted at Surgical Unit-I Civil Hospital Karachi, March 2013 to August 2013. Methodology: A total of 281 patients diagnosed to have gall stone on basis of clinical history of pain in right upper abdomen with fatty intolerance confirmed on ultrasoundwho were operated rather by laparoscopic procedure or open surgery were included in the study. Immediately following gallbladder removal, the specimen was collected in a sterile cup. The specimen was sent to Laboratory for presence of H. Pylori using GEIMSA staining and for routine histopathology. Data was collected by a resident in predesigned proforma. Results: A total of 281 Patients diagnosed to have gall stone. Operated most of the cases were 21 to 50 years of age with mean age was 38.41±9.86 years. Out of 281 cases, 170 (60.5%) were female and 111 (39.5%) male. Seventy eight patients (27.76%) was observed obsess (BMI > 30kg/m2). Similarly duration of illness of 58.4% was below and equal to 6 months and 41.6% cases above six months. Frequency of helicobacter pylori in gallbladder of patients with gall stones disease was positive in 72(25.62%) cases. Conclusion: We conclude that H. pylori are also found in the biliary system, suggesting that these bacteria are of etiological importance in gallstoneformation. Early detection of helicobacter pylori and its eradication can prevent development of gall stones.

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Identification of Helicobacter spp. in bile and gallbladder tissue of patients with symptomatic gallbladder disease

Abstract: Helicobacter spp. can be detected in bile and gallbladder tissue of patients with benign gallbladder disease. The contribution of these bacteria to the pathophysiology of gallbladder disease and gallstone formation requires further study.

Cited by 12 publications

References 24 publications

Interactions between Bacteria and Bile Salts in the Gastrointestinal and Hepatobiliary Tracts

Interactions between Bacteria and Bile Salts in the Gastrointestinal and Hepatobiliary Tracts

Helicobacter apodemussp. nov., a newHelicobacterspecies identified from the gastrointestinal tract of striped field mice in Korea

Helicobacter Pylori

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