Identification of heat-dissociable RNA complexes in two porcine coronaviruses

Garwes, D. J.; Pocock, D. H.; Wijaszka, T.

doi:10.1038/257508a0

Cited by 26 publications

(16 citation statements)

References 5 publications

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“…More recently, Watkins et al (1975) scribed IBV RNA, after its release from virions by SDS lysis, as a single species having a molecular weight of 9.0 x lo6 and a sedimentation coefficient of 50 S. They reported, but without supporting evidence, that by phenol extraction a range of RNA fragments could be obtained which was similar to that Tannock (1973) described. Garwes et al (1975) examined the RNAs of two porcine coronaviruses, transmissible gastroenteritis virus (TGEV) and hemagglutinating encephalomyelitis virus (HEV), after preparation by SDS lysis. They obtained a single large RNA peak with electrophoretic mobility similar to that of 70 S Rous sarcoma virus (RSV) RNA.…”

Section: Introductionmentioning

confidence: 99%

The RNA of human coronavirus OC-43

Tannock

Hierholzer

1977

Virology

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Section: Introductionmentioning

confidence: 99%

The RNA of human coronavirus OC-43

Tannock

Hierholzer

1977

Virology

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“…wt (9.0 X 106) RNA species. Another report [6] has shown that the coronaviruses, transmissible gastroenteritis virus and haemagglutinating encephalomyelitis virus also contain single large molecular weight RNA genomes. The present report describes a method of isolation of IBV-RNA, using proteinase K, that reveals a single single-stranded high mol.…”

Section: Wimentioning

confidence: 99%

The characterisation of the virion RNA of avian Infectious Bronchitis Virus

Macnaughton¹,

Madge²

1977

FEBS Letters

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“…A single species of glycopolypeptide is associated with the projection while three major polypeptides, of which two are glycosylated, make up the remainder of the virus structural protein (Garwes & Pocock, 1975).…”

Section: Introductionmentioning

confidence: 99%

Isolation of Subviral Components from Transmissible Gastroenteritis Virus

Garwes¹,

Pocock²,

Pike³

1976

Journal of General Virology

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SUMMARYExposure of purified transmissible gastroenteritis virus, a porcine coronavirus, to non-ionic detergents resulted in the removal of the surface projections and > 98 % of the virus lipid. Virus RNA was associated with a subviral particle which had a sedimentation coefficient of 65olS, compared with 495 S for the intact virion, and which banded in Cs2SO4 gradients at 1.295 g/ml. Negatively stained preparations of subviral particles were shown by electron microscopy to contain spherical particles of 6o to 7o nm diam., similar in appearance to those derived from oncornaviruses.Polyacrylamide gel electrophoresis of the polypeptides from isolated subviral particles showed that these structures contained three of the four major virus structural proteins, the arginine-rich polypeptide VP2 and the two membrane glycopolypeptides VP3 and 4. The detergent-liberated surface projections, composed of a single species of sulphated glycopolypeptide, VPr, were isolated by rate-zonal centrifugation through sucrose gradients followed by precipitation with ammonium sulphate in the presence of bovine serum albumin.

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Identification of heat-dissociable RNA complexes in two porcine coronaviruses

Cited by 26 publications

References 5 publications

The RNA of human coronavirus OC-43

The RNA of human coronavirus OC-43

The characterisation of the virion RNA of avian Infectious Bronchitis Virus

Isolation of Subviral Components from Transmissible Gastroenteritis Virus

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