The RNA of human coronavirus OC-43

Tannock, Gregory A.; Hierholzer, John C.

doi:10.1016/0042-6822(77)90126-x

Cited by 17 publications

(18 citation statements)

References 32 publications

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“…single single-stranded RNA species. Similar observations have been made with the coronaviruses: IBV (Watkins et aL 1975;Lomniczi, I977;Macnaughton & Madge, 1977;Schochetman et al I977), TGEV (Garwes et al I975) and HCV OC43 (Tannock & Hierholzer, 1977).…”

Section: Polynucleotide Phosphorylase Digestion Of Hcv 229e Rnasupporting

confidence: 59%

“…Tannock (I973) and Watkins et aL (I975), using IBV, and Tannock & Hierholzer (1977), using HCV OC43, have shown that coronavirus RNA extracted with phenol was very heterogeneous. Furthermore, Garwes et aL (1975) and Tannock & Hierholzer (1977) observed that heating TGEV and HCV OC43 RNAs, respectively, dissociated the RNA molecules and material of about 4S accumulated. However, Watkins et al (I975), Macnaughton & Madge (1977), Schochetman et aL (1977) and Lomniczi (1977) have observed no genome degradation on heating IBV RNA.…”

Section: Polynucleotide Phosphorylase Digestion Of Hcv 229e Rnamentioning

confidence: 99%

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The Genome of Human Coronavirus Strain 229E

Macnaughton¹,

Madge²

1978

Journal of General Virology

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SUMMARYThe genomic RNA of human coronavirus strain 229E (HCV 229E) migrated on polyacrylamide gels as a single peak with a tool. wt. of 5"8 x IO 6. Denaturation of the genome with formaldehyde did not alter its electrophoretic mobility, which suggests that the I-ICV Ee9E genome is a single-stranded molecule. At least 3o ~ of the genomic RNA was shown to contain covalently attached polyadenylic acid [poly(A)] sequences by binding the RNA to an oligo(dT)-cellulose column. These poly(A) tracts were shown to be about 7o nucleotides in length by measuring the resistance to digestion of HCV 229E RNA with pancreatic and T1 RNases. Finally, the genomic RNA was shown to terminate at or near the 3'-terminus on the basis of its susceptibility to polynucleotide phosphorylase.

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Section: Polynucleotide Phosphorylase Digestion Of Hcv 229e Rnasupporting

confidence: 59%

Section: Polynucleotide Phosphorylase Digestion Of Hcv 229e Rnamentioning

confidence: 99%

The Genome of Human Coronavirus Strain 229E

Macnaughton¹,

Madge²

1978

Journal of General Virology

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“…This shows that the coronavirus genomes are not composed of multiple subunits and contain little, if any, double strandedness. Some reports suggest that heating or denaturation can produce an alteration in the size or migration of the RNAs [5,6,8] . This may be due to contamination of the RNAs by an activated retrovirus or the presence of an internal ribonuclease [4] or the nicking of the large RNAs during isolation.…”

Section: Methodsmentioning

confidence: 99%

“…wt varying from 6.1 X lo6 for strain OC43 [6] to 5.8 X IO6 for strain 2298 [7]. Again, as with IBV, the HCV genome is prone to degradation [6,7]. The genomes of two porcine coronaviruses, transmissible gastroenteritis virus (TGEV) and haemagglutinating encephalomyelitis virus (HEV), have also been shown to be large single single-stranded RNA molecules of about 60 S that dissociate into 35 S and 4 S material on heating above 60°C [8] ~ Coronavirus genomes possess certain mRNA characteristics.…”

Section: Introductionmentioning

confidence: 99%