Identification of glucosinolates on the leaf surface of plants from the Cruciferae and other closely related species

Griffiths, D. W.; Deighton, Nigel; Birch, Andrew Nicholas; Patrian, Bruno; Baur, Robert; Städler, Erich

doi:10.1016/s0031-9422(01)00138-8

Cited by 115 publications

(86 citation statements)

References 28 publications

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“…Comparative analyses of the glucosinolates at the surface of various crucifers and related plants were recently used to demonstrate correlations of profiles with host preferences of the cabbage root fly. However, the results of this study further suggested that additional compounds are involved in regulating oviposition by this insect (Griffiths et al, 2001). It appears likely, therefore, that many compounds in crucifers that could be used by specialist insects to recognize their host plants remain to be identified.…”

Section: Sensory Aspects Of Host Selectionmentioning

confidence: 60%

The chemical world of crucivores: lures, treats and traps

Renwick

2002

Entomologia Exp Applicata

166

102

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The host ranges of several insects that are specialists on crucifers (Brassicaceae) are closely linked to the presence of glucosinolates in these plants. These glycosides often serve as stimulants for oviposition and/or feeding, while their volatile hydrolysis products may be attractants for several species. However, many crucifers produce additional secondary compounds that act as repellents, deterrents or toxins, which protect them from these insects. The widely different responses of the various crucifer specialists to these compounds reflect different degrees of adaptation to the plant defenses. Thus native insects are often unable to survive on introduced plants, although the ubiquitous glucosinolates may trigger oviposition 'mistakes'. The success of highly invasive cruciferous weeds may be due in part to a lack of local herbivore adaptation to unique chemical constituents of these plants. However, the concentrations of secondary chemicals vary with season, environmental conditions, and geographical location. This could mean that windows of opportunity exist for utilization of introduced plants. Recent studies with garlic mustard, Alliaria petiolata, and wintercress, Barbarea vulgaris, in the USA have shown that these introduced plants are resistant to the native butterfly, Pieris napi oleracea. The combined effects of a flavone glycoside and a unique butenenitrile glycoside in the garlic mustard appear to be responsible for blocking feeding by this insect. Barbarea vulgaris is also resistant to the diamondback moth, Plutella xylostella, in North America and to the flea beetle, Phyllotreta nemorum, in Europe. Comparative studies indicate that common resistance mechanisms are involved and bioassays have been developed to elucidate the chemical nature of this resistance.

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Section: Sensory Aspects Of Host Selectionmentioning

confidence: 60%

The chemical world of crucivores: lures, treats and traps

Renwick

2002

Entomologia Exp Applicata

166

102

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“…An aspect of glucosinolate localization in plants that has been controversial in recent years is whether or not these compounds are found on the leaf surface (32,33). The presence of glucosinolates on the surface would supply important cues for insect host choice.…”

Section: Discussionmentioning

confidence: 99%

Nonuniform distribution of glucosinolates in Arabidopsis thaliana leaves has important consequences for plant defense

Shroff

Vergara

Muck

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

256

236

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The spatial distribution of plant defenses within a leaf may be critical in explaining patterns of herbivory. The generalist lepidopteran larvae, Helicoverpa armigera (the cotton bollworm), avoided the midvein and periphery of Arabidopsis thaliana rosette leaves and fed almost exclusively on the inner lamina. This feeding pattern was attributed to glucosinolates because it was not evident in a myrosinase mutant that lacks the ability to activate glucosinolate defenses by hydrolysis. To measure the spatial distribution of glucosinolates in A. thaliana leaves at a fine scale, we constructed ion intensity maps from MALDI-TOF (matrix assisted laser desorption/ionization-time of flight) mass spectra. The major glucosinolates were found to be more abundant in tissues of the midvein and the periphery of the leaf than the inner lamina, patterns that were validated by HPLC analyses of dissected leaves. In addition, there were differences in the proportions of the three major glucosinolates in different leaf regions. Hence, the distribution of glucosinolates within the leaf appears to control the feeding preference of H. armigera larvae. The preferential allocation of glucosinolates to the periphery may play a key role in the defense of leaves by creating a barrier to the feeding of chewing herbivores that frequently approach leaves from the edge.antiherbivore defense ͉ MALDI-imaging ͉ plant natural products M any plant natural products appear to serve as defenses against herbivores because of their toxicity or deterrence in artificial diets or when added as a supplement to plant material (1, 2). However, to evaluate the actual defensive role of these substances in planta, it is necessary to estimate the amount that a potential herbivore would encounter during feeding. Although determining the amount of plant tissue ingested during a feeding bout is relatively straightforward, measuring the quantity of specific defense products in that tissue is not. Nearly all studies to date have quantified the levels of defensive metabolites at the level of the whole plant or organ (3). Little is known about the localization of antiherbivore defenses in individual tissues or parts of organs even though this may be critically important to the behavior of small herbivores and to the effectiveness of the defense.One of the most extensively studied classes of antiherbivore chemical defenses in plants is the glucosinolates, a group of sulfur-rich, amino acid-derived metabolites combining a ␤-Dglucopyranose residue linked via a sulfur atom to an Nhydroxyamino sulfate ester (4) (Fig. 1). Glucosinolates are widespread in the order Capparales, which includes vegetables (cabbage, cauliflower, and broccoli), spice plants supplying condiments (mustard, horseradish, and wasabi), and the model species, Arabidopsis thaliana (5, 6). Upon insect feeding or mechanical disruption, glucosinolates are hydrolyzed by an endogenous glucohydrolase activity known as myrosinase, and the released aglycone rearranges to form isothiocyanates, nitriles, and other pr...

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“…Glucosinolatos (benzilglucosinolatos), como glucotropaeolina sinalbina e os triterpenos tetracíclicos cucurbitacinos foram isolados previamente das folhas desta planta (LYKKESFELDT & MELLER, 1993;GRIFFITHS et al, 2001). Diante do potencial alelopático inibitório das folhas de capuchinha e da presença de compostos com tais propriedades sugere-se a realização de novas pesquisas como a purificação das frações e o isolamento dos compostos para a confirmação da atividade fitotóxica das folhas de capuchinha sobre o estabelecimento de picão-preto.…”

Section: Resultsunclassified