Identification of Ginsenosides in Panax quinquefolium by LC-MS

Ma, Xin; Xiao, Hang; Liang, Xinmiao

doi:10.1365/s10337-006-0812-z

Cited by 36 publications

(32 citation statements)

References 18 publications

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“…Ginsenoside Re and Rg 1 , two not easily separated ginsenosides in most previous studies [26,32,33], co-eluted under the present chromatographic conditions. Their deprotonated ions [M-H] − and low energy CID fragment ions (such as ion m/z 783.49 of Re) were used to confirm the existence of these two ginsenosides in WG.…”

Section: Identity Assignment and Confirmation Of The Detected Ginsenocontrasting

confidence: 50%

“…Five extra peaks (peaks 21, 22, 25, 26, and 29) were detected in the mimic nine cycles of steaming extracts of ginsenosides Rb 1 and Rc (Figs. 3, A2, and B2), and were identified as 20(S)-Rg 3 , 20(R)-Rg 3 , Rk 1 , Rg 5 , and Rh 2 , respectively [33]. The mechanisms involved were deduced to be the loss of glycosyl moiety at C-20-OH of Rb 1 and Rc through hydrolysis to generate the stereoisomers 20(S)-Rg 3 and 20(R)-Rg 3 [40,41].…”

Section: Possible Mechanisms Involved In the Nine Cycles Of Steaming-mentioning

confidence: 99%

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Repetitious steaming-induced chemical transformations and global quality of black ginseng derived from Panax ginseng by HPLC-ESI-MS/MSn based chemical profiling approach

Sun

Pan

Sung

2011

Biotechnol Bioproc E

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A high performance liquid chromatographyelectrospray tandem mass spectrometry (HPLC-ESI-MS/ MS n ) based chemical profiling method was developed to evaluate repetitious steaming-induced chemical transformations in black ginseng (BG and Korean white ginseng subjected to nine cycles of steam treatment). Under the optimized HPLC and ESI-MS/MS n conditions, more than 13 and 17 peaks were separated and detected in white ginseng (WG) and BG within 85 min, respectively. The components were identified by comparing the mass spectrum and/or matching the empirical molecular formula with that of known published compounds. In total, 17 major ginsenosides were identified in BG, 16 of which were determined to be newly generated during the BG preparatory process. The mechanisms involved were further deduced to be hydrolysis, dehydration, isomerization, and decarboxylation reactions of the original ginsenosides in WG by analyzing nine mimic cycles of steaming extracts of seven pure reference ginsenosides. A significant difference in chemical profiles between BGs developed from two batches of WG suggested that storage duration significantly influenced the quality consistency of not only the crude drug but also the BG derived from WG.

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Section: Identity Assignment and Confirmation Of The Detected Ginsenocontrasting

confidence: 50%

Section: Possible Mechanisms Involved In the Nine Cycles Of Steaming-mentioning

confidence: 99%

Repetitious steaming-induced chemical transformations and global quality of black ginseng derived from Panax ginseng by HPLC-ESI-MS/MSn based chemical profiling approach

Sun

Pan

Sung

2011

Biotechnol Bioproc E

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“…The coupling of HPLC and MS has helped online structural characterization and rapid identification of compounds in complex herbal extracts 170,206,207,242. The application of tandem MS or, more recently, IT-TOF-MS and Q-TOF-MS, also has advanced the online identification of constituents in ginseng samples 186,187,190,243,244…”

Section: Applications and Challengesmentioning

confidence: 99%

“…Online structure characterization has been used to identify 152 saponins from P. notoginseng by LC/ESI-MS n ;188 35 ginseng saponins in P. ginseng 187 and 30 ginseng saponins in P. quinquefolius 186 were identified by LC-APCI-MS.…”

Section: Applications and Challengesmentioning

confidence: 99%

“…Malonyl-ginsenoside Ra372 and malonyl-notoginsenoside R473 were recently isolated from the fresh roots of P. ginseng . Approximately fifteen malonyl ginseng saponins were found in ginseng extract by HPLC-MS/MS 186,187,194,244. Malonyl ginseng saponins, more polar and water soluble than neutral ginseng saponins, are extremely unstable and susceptible to thermal degradation during drying, storage, heating and extraction 29,247,248.…”

Section: Applications and Challengesmentioning

confidence: 99%

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Isolation and analysis of ginseng: advances and challenges

2011

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Ginseng occupies a prominent position in the list of best-selling natural products in the world. Because of its complex constituents, multidisciplinary techniques are needed to validate the analytical methods that support ginseng's use worldwide. In the past decade, rapid development of technology has advanced many aspects of ginseng research. The aim of this review is to illustrate the recent advances in the isolation and analysis of ginseng, and to highlight their new applications and challenges. Emphasis is placed on recent trends and emerging techniques. The current article reviews

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Current literature in mass spectrometry

2007

J. Mass Spectrom.

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Identification of Ginsenosides in Panax quinquefolium by LC-MS

Cited by 36 publications

References 18 publications

Repetitious steaming-induced chemical transformations and global quality of black ginseng derived from Panax ginseng by HPLC-ESI-MS/MSn based chemical profiling approach

Repetitious steaming-induced chemical transformations and global quality of black ginseng derived from Panax ginseng by HPLC-ESI-MS/MSn based chemical profiling approach

Isolation and analysis of ginseng: advances and challenges

Current literature in mass spectrometry

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