Identification of genes expressed in human CD34⁺hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning

Abstract

ABSTRACT

ATPase-defective Mammalian VPS4 Localizes to Aberrant Endosomes and Impairs Cholesterol Trafficking

“…The cDNA was then resequenced in the reverse direction and subsequently deposited on the database (GenBank accession number AF038960). Compared with the full-length DNA sequence of human SKD1 (Mao et al, 1998), hVPS4 lacks the first seven N-terminal amino acids.…”

“…Comparison with a mouse Vps4p-related protein, mSKD1 (Périer et al, 1994), and human SKD1 (designated hSKD1; Mao et al, 1998), indicated that the hVPS4 insert was missing the first seven amino acids of the protein (Figure 1). For our studies we used cDNA constructs containing this truncated hVPS4 or full-length mouse SKD1.…”

A method for identifying differentially expressed genes in rare populations of primary human hematopoietic cells

“…[15][16][17][18] Ross et al 15 reported a screening strategy similar to the one we report using cDNA amplified from adaptor-ligated ds cDNA. The method described here has several advantages over the method of Ross et al in that isolation of polyA RNA was not necessary for our cDNA amplifications.…”

Identification of a Mouse Synaptic Glycoprotein Gene in Cultured Neurons

“…4). Moreover, there are two visible PCR bands in most non-neural tissues, except testis, indicating a possible presence of at least two splice variants of the mSC2 transcript, like the human mSC2 homolog (9). In fact, the extra PCR band observed in various tissues was subjected to DNA sequencing, and the obtained sequence was compared with mSC2 and other mammalian SC2 (Fig.…”

“…Vector sequences and ''trash'' sequences, defined as sequences from bacterial sequences and primer polymers, sequences containing >3% of ambiguous bases, and sequences <100 bp long, were removed. The searched results were re-confirmed against the GenBank database for sequence similarity (9).…”