Identification of a Mouse Synaptic Glycoprotein Gene in Cultured Neurons

Abstract

Neuronal differentiation and aging are known to involve many genes, which may also be differentially expressed during these developmental processes. From primary cultured cerebral cortical neurons, we have previously identified various differentially expressed gene transcripts from cultured cortical neurons using the technique of arbitrarily primed PCR (RAP-PCR). Among these transcripts, clone 0-2 was found to have high homology to rat and human synaptic glycoprotein. By in silico analysis using an EST database and the FACTURA software, the full-length sequence of 0-2 was assembled and the clone was named as mouse synaptic glycoprotein homolog 2 (mSC2). DNA sequencing revealed transcript size of mSC2 being smaller than the human and rat homologs. RT-PCR indicated that mSC2 was expressed differentially at various culture days. The mSC2 gene was located in various tissues with higher expression in brain, lung, and liver. Functions of mSC2 in neurons and other tissues remain elusive and will require more investigation.

Involvement of Microglia Activation in the Lead Induced Long-Term Potentiation Impairment

“…Rat primary hippocampal cultures were prepared following a previously described protocol [17], [18], [19] with some modifications. Briefly, hippocampal tissues were dissected from postnatal day 1 SD rats and dissociated mechanically without using enzymes.…”