Identification of Genes Differentially Expressed in the Ganglia of Growing<i>Haliotis asinina</i>

York, Patrick S.; Cummins, Scott F.; Degnan, Sandie M.; Woodcroft, Ben J.; Degnan, Bernard M.

doi:10.2983/035.029.0328

Cited by 5 publications

(7 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two partial-length sequences encoding the Has-Myomodulin transcript were found in the RA/NRA SSH library. Has-Myomodulin sequence was extended in silico by alignment with previously discovered expressed sequence tags (ESTs) ([GenBank:GT277631, GenBank:GT275969, GenBank:GT273228, GenBank:GT067419, GenBank:GT067304]; [ 36 , 37 ]). The isolated final sequence length was 1526 bp (Figure 2 A, [GenBank:JN606062]), encoding a well-conserved 297 residue prepropeptide (Figure 2 B), which was most similar to the A. californica Myomodulin 1 prepropeptide (BLASTp E-value 2e-49) [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

“…Myomodulins also have a well-documented role in feeding [ 50 - 55 ]. Interestingly, we have previously shown that expression of Has-Myomodulin in the anterior ganglia is also correlated with feeding status in aquaculture [ 36 ]. Indeed, variation in individual foraging success may explain the high individual variation in expression of this gene in this study.…”

Section: Discussionmentioning

confidence: 99%

“…The HGAP gene was first found in a H. asinina anterior ganglia SSH library created from well-nourished against food-deprived animals ([ 36 ]; [GenBank:GT067343]). Further characterisation has revealed that Has-HGAP encodes a double-chain secreted peptide and is expressed in all tissues examined except the gill [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

“…RNA isolation, cDNA synthesis and amplification, SSH, cloning, sequencing, and in silico sequence extension were carried out as described in York et al 2010 [ 36 ]. SSH utilised anterior ganglia from two reproductively active (RA) and two non-reproductively active (NRA) adult H. asinina as Tester and Driver samples, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…For each point in the time series, anterior ganglia were dissected from 4 anaesthetised male and female abalone, each which was haphazardly chosen from the pool of previously collected gravid adults, and stored in RNALater at −80°C as previously described [ 36 ]. Total RNA was extracted using Tri Reagent (Sigma-Aldrich, St. Louis, Missouri), and reverse transcribed using SuperScript III Reverse Transcriptase (Invitrogen, Carlsbad, California), as per manufacturers’ instructions.…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Marked changes in neuropeptide expression accompany broadcast spawnings in the gastropod Haliotis asinina

et al. 2012

View full text Add to dashboard Cite

IntroductionA huge diversity of marine species reproduce by synchronously spawning their gametes into the water column. Although this species-specific event typically occurs in a particular season, the precise time and day of spawning often can not be predicted. There is little understanding of how the environment (e.g. water temperature, day length, tidal and lunar cycle) regulates a population’s reproductive physiology to synchronise a spawning event. The Indo-Pacific tropical abalone, Haliotis asinina, has a highly predictable spawning cycle, where individuals release gametes on the evenings of spring high tides on new and full moons during the warmer half of the year. These calculable spawning events uniquely allow for the analysis of the molecular and cellular processes underlying reproduction. Here we characterise neuropeptides produced in H. asinina ganglia that are known in egg-laying molluscs to control vital aspects of reproduction.ResultsWe demonstrate that genes encoding APGWamide, myomodulin, the putative proctolin homologue whitnin, FMRFamide, a schistosomin-like peptide (SLP), a molluscan insulin-related peptide (MIP) and a haliotid growth-associated peptide (HGAP) all are differentially expressed in the anterior ganglia during the two week spawning cycle in both male and female abalone. Each gene has a unique and sex-specific expression profile. Despite these differences, expression levels in most of the genes peak at or within 12 h of the spawning event. In contrast, lowest levels of transcript abundance typically occurs 36 h before and 24 h after spawning, with differences in peak and low expression levels being most pronounced in genes orthologous to known molluscan reproduction neuromodulators.ConclusionsExploiting the predictable semi-lunar spawning cycle of the gastropod H. asinina, we have identified a suite of evolutionarily-conserved, mollusc-specific and rapidly-evolving neuropeptides that appear to contribute to the regulation of spawning. Dramatic increases and decreases in ganglionic neuropeptide expression levels from 36 h before to 24 h after the broadcast spawning event are consistent with these peptides having a regulatory role in translating environmental signals experienced by a population into a synchronous physiological output, in this case, the release of gametes.

show abstract

Section: Resultsmentioning

confidence: 99%