Differential expression of neuropeptides correlates with growth rate in cultivated Haliotis asinina (Vetigastropoda: Mollusca)

York, Patrick S.; Cummins, Scott F.; Lucas, Tim; Blomberg, Simon P.; Degnan, Sandie M.; Degnan, Bernard M.

doi:10.1016/j.aquaculture.2011.12.019

Cited by 9 publications

(16 citation statements)

References 57 publications

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“…Multiple individual peptide neuromodulators are derived from a common propeptide precursor by post-translational cleavage and subsequent modification [ 43 ]. As for other H. asinina neuropeptides, namely Has-SLP, Has-MIP, Has-HGAP [ 44 ] and Has-FMRFamide [ 45 ], we demonstrate here that prohormones encoded by Has-Myomodulin, Has-APGWamide and Has-Whitnin are processed to yield a repertoire of small neuropeptides. The Has-Myomodulin gene encodes 7 copies of the highly conserved PMNMLRLamide, compared to 9 to 10 copies of the corresponding myomodulin in other characterised gastropods [ 34 , 38 , 39 ].…”

Section: Discussionsupporting

confidence: 71%

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Marked changes in neuropeptide expression accompany broadcast spawnings in the gastropod Haliotis asinina

et al. 2012

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IntroductionA huge diversity of marine species reproduce by synchronously spawning their gametes into the water column. Although this species-specific event typically occurs in a particular season, the precise time and day of spawning often can not be predicted. There is little understanding of how the environment (e.g. water temperature, day length, tidal and lunar cycle) regulates a population’s reproductive physiology to synchronise a spawning event. The Indo-Pacific tropical abalone, Haliotis asinina, has a highly predictable spawning cycle, where individuals release gametes on the evenings of spring high tides on new and full moons during the warmer half of the year. These calculable spawning events uniquely allow for the analysis of the molecular and cellular processes underlying reproduction. Here we characterise neuropeptides produced in H. asinina ganglia that are known in egg-laying molluscs to control vital aspects of reproduction.ResultsWe demonstrate that genes encoding APGWamide, myomodulin, the putative proctolin homologue whitnin, FMRFamide, a schistosomin-like peptide (SLP), a molluscan insulin-related peptide (MIP) and a haliotid growth-associated peptide (HGAP) all are differentially expressed in the anterior ganglia during the two week spawning cycle in both male and female abalone. Each gene has a unique and sex-specific expression profile. Despite these differences, expression levels in most of the genes peak at or within 12 h of the spawning event. In contrast, lowest levels of transcript abundance typically occurs 36 h before and 24 h after spawning, with differences in peak and low expression levels being most pronounced in genes orthologous to known molluscan reproduction neuromodulators.ConclusionsExploiting the predictable semi-lunar spawning cycle of the gastropod H. asinina, we have identified a suite of evolutionarily-conserved, mollusc-specific and rapidly-evolving neuropeptides that appear to contribute to the regulation of spawning. Dramatic increases and decreases in ganglionic neuropeptide expression levels from 36 h before to 24 h after the broadcast spawning event are consistent with these peptides having a regulatory role in translating environmental signals experienced by a population into a synchronous physiological output, in this case, the release of gametes.

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Section: Discussionsupporting

confidence: 71%

“…Further characterisation has revealed that Has-HGAP encodes a double-chain secreted peptide and is expressed in all tissues examined except the gill [44]. To date, HGAP has only been found in H. asinina .…”

Section: Discussionmentioning

confidence: 99%

Marked changes in neuropeptide expression accompany broadcast spawnings in the gastropod Haliotis asinina

et al. 2012

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“…The schistosomin-like, pleurin and enterin neuropeptides appear to have a more defined presence within the molluscan classes, specific to the gastropods. The schistosomin-like neuropeptide gene is known to be upregulated within fast growing tropical abalone ( Haliotis asinina ) (York et al 2012b). Excluding the H. laevigata ganglia, 15 NPP genes show relatively high normalized expression in several other tissues, including the buccal, hepatopancreas, gill, stomach, radula, sensory tentacles (cephalic and epipodial), and foot muscle tissues (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

Greenlip Abalone (Haliotis laevigata) Genome and Protein Analysis Provides Insights into Maturation and Spawning

Botwright

Zhao

Wang

et al. 2019

G3 Genes|Genomes|Genetics

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Wild abalone (Family Haliotidae) populations have been severely affected by commercial fishing, poaching, anthropogenic pollution, environment and climate changes. These issues have stimulated an increase in aquaculture production; however production growth has been slow due to a lack of genetic knowledge and resources. We have sequenced a draft genome for the commercially important temperate Australian ‘greenlip’ abalone (Haliotis laevigata, Donovan 1808) and generated 11 tissue transcriptomes from a female adult abalone. Phylogenetic analysis of the greenlip abalone with reference to the Pacific abalone (Haliotis discus hannai) indicates that these abalone species diverged approximately 71 million years ago. This study presents an in-depth analysis into the features of reproductive dysfunction, where we provide the putative biochemical messenger components (neuropeptides) that may regulate reproduction including gonad maturation and spawning. Indeed, we isolate the egg-laying hormone neuropeptide and under trial conditions induce spawning at 80% efficiency. Altogether, we provide a solid platform for further studies aimed at stimulating advances in abalone aquaculture production. The H. laevigata genome and resources are made available to the public on the abalone ‘omics website, http://abalonedb.org.

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“…Identification of basic elements of endocrine and regulatory networks of vertebrates indicated that a similar system could exist in marine mollusks. Growth rate of abalones has been associated with three neuropeptides secreted in neural ganglia (York et al, 2012), supporting the neural control of growth. Many studies in mollusks have also reported the association of genetic mechanisms within insulin-related peptide genes with differences in growth (Kellner-Cousin et al, 1994; Gricourt et al, 2003; Cong et al, 2013; Feng et al, 2014; Alarcon-Matus et al, 2015).…”

Section: Introductionmentioning

confidence: 91%

Comparative Transcriptome Analysis Reveals Molecular Basis Underlying Fast Growth of the Selectively Bred Pacific Oyster, Crassostrea gigas

Zhang

et al. 2019

Front. Genet.

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Fast growth is one of the most desired traits for all food animals, which affects the profitability of animal production. The Pacific oyster, Crassostrea gigas , is an important aquaculture shellfish around the world with the largest annual production. Growth of the Pacific oyster has been greatly improved by artificial selection breeding, but molecular mechanisms underlying growth remains poorly understood, which limited the molecular integrative breeding of fast growth with other superior traits. In this study, comparative transcriptome analyses between the fast-growing selectively bred Pacific oyster and unselected wild Pacific oysters were conducted by RNA-Seq. A total of 1,303 protein-coding genes differentially expressed between fast-growing oysters and wild controls were identified, of which 888 genes were expressed at higher levels in the fast-growing oysters. Functional analysis of the differentially expressed genes (DEGs) indicated that genes involved in microtubule motor activity and biosynthesis of nucleotides and proteins are potentially important for growth in the oyster. Positive selection analysis of genes at the transcriptome level showed that a significant number of ribosomal protein genes had undergone positive selection during the artificial selection breeding process. These results also indicated the importance of protein biosynthesis and metabolism for the growth of oysters. The alternative splicing (AS) of genes was also compared between the two groups of oysters. A total of 3,230 differential alternative splicing events (DAS) were identified, involved in 1,818 genes. These DAS genes were associated with specific functional pathways related to growth, such as “long-term potentiation,” “salivary secretion,” and “phosphatidylinositol signaling system.” The findings of this study will be valuable resources for future investigation to unravel molecular mechanisms underlying growth regulation in the oyster and other marine invertebrates and to provide solid support for breeding application to integrate fast growth with other superior traits in the Pacific oyster.

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Differential expression of neuropeptides correlates with growth rate in cultivated Haliotis asinina (Vetigastropoda: Mollusca)

Cited by 9 publications

References 57 publications

Marked changes in neuropeptide expression accompany broadcast spawnings in the gastropod Haliotis asinina

Marked changes in neuropeptide expression accompany broadcast spawnings in the gastropod Haliotis asinina

Greenlip Abalone (Haliotis laevigata) Genome and Protein Analysis Provides Insights into Maturation and Spawning

Comparative Transcriptome Analysis Reveals Molecular Basis Underlying Fast Growth of the Selectively Bred Pacific Oyster, Crassostrea gigas

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