Identification of genes associated with cold acclimation in perennial ryegrass

Differences in leaf proteome response to cold acclimation between Lolium perenne plants with distinct levels of frost tolerance

“…Comprehensive research on the transcriptome and the proteome (Kosmala et al, 2009) during cold treatment has been recently shown for Fp, the most frost tolerant species within the Lolium-Festuca complex. Identification of genes associated with CA in Lp based on transcriptome screening has recently been performed by Zhang et al (2009). Here, we present the first complex research on Lp proteome during CA to test the hypothesis that the proteomic response to cold treatment varies between Lp genotypes representing different levels of FT.…”

Fructan accumulation and transcription of candidate genes during cold acclimation in three varieties of Poa pratensis

“…Cold regulated proteins are known to be induced during cold acclimation (Zhang et al, 2009) and many such proteins, including CAP, contain several membrane spanning domains. Coldinduced transcription of CAP may regulate or assist plant cells to withstand cold and freezing conditions, possibly by protecting the membranes (Breton et al, 2003;Zhang et al, 2009). In agreement with this, PpCAP transcription increased during cold acclimation in all three varieties.…”

“…In addition, FEHs have been suggested to mediate freezing tolerance by degrading fructans, thereby generating a mixture of sugars to regulate the osmotic potential of the cell and stabilize the cell membrane during freezing Van den Ende et al, 2005). Fructans may work alongside cold acclimation proteins (CAP) -cold regulated multi-spanning transmembrane proteins -which are also known to mediate freezing tolerance in the plant cell membrane (Breton et al, 2003;Zhang et al, 2009). P. pratensis L. (also known as Kentucky bluegrass and in the following denoted as P. pratensis) is an important cool season forage and turf grass that reproduces both sexually, by outcrossing and selfing, and apomictically.…”

Molecular and Genetic Analysis of Abiotic Stress Resistance of Forage Crops

“…Cold acclimation includes the expression of certain cold-induced genes that function to stabilize membranes against freeze-induced injury (Thomashow, 1999). In forage grasses, many genes (Mohapatra et al, 1989;Tamura & Yonemaru, 2010;Tominaga et al, 2001;Zhang et al, 2009) and proteins (Kosmala et al, 2009) are regulated during cold acclimation. In alfalfa, some CAS (cold-acclimation-specific) genes are specifically expressed during coldacclimation and their expression level measured by mRNA abundance is positively correlated with the freezing-tolerance of cultivars (Mohapatra et al, 1989).…”