Differences in leaf proteome response to cold acclimation between Lolium perenne plants with distinct levels of frost tolerance

Bocian, Aleksandra; Kosmala, Arkadiusz; Rapacz, Marcin; Jurczyk, Barbara; Marczak, Łukasz; Zwierzykowski, Z.

doi:10.1016/j.jplph.2011.01.029

Cited by 47 publications

(40 citation statements)

References 28 publications

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“…The focus on studies with forage crops centres on the improvement of stress tolerance in different climates. A study on the effects of cold and frost on the proteome of Lolium perenne showed differences in the leaf proteomes of high and low frost tolerance (Bociana et al 2011 ). Forty-two differentially abundant proteins were identifi ed of which 35 were found to be chloroplast proteins, suggesting resistance to photo-inhibition and survival during cold acclimatization through continued food manufacture.…”

Section: Forage Crop Proteomicsmentioning

confidence: 99%

Translating the Genome for Translational Research: Proteomics in Agriculture

Caguioa

Raorane

Kohli

2015

Plant Biology and Biotechnology

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The increasing number of novel tools, methods and capacities to elucidate and understand the genome of various organisms is steadily leading the path for similar groundbreaking studies in downstream comprehension of the genomes. Platforms for analytical and functional transcriptomics have seen a similar surge in operational outputs. The cryptic complexity of the DNA in the genomes is manifested in various forms and functions of the RNA it codes for. Such complexity reaches much more elaborate intricacies in the translational protein products of the RNA. Although transcripts regulate a number of biological processes, proteins form the basic functional unit of most procedures that are evinced as a phenotype. Progressively increasing realization that there is limited correspondence between the level of a specifi c transcript and protein is only the beginning of appreciating that predictive biology would be less dependent on transcripts than on proteins. Principles of protein function that were explored much before the DNA and RNA paradigms are coming back to inform the omics world in the functional and regulatory capacity of proteins. Combined with the evolution of the high-throughput analytical capacity for proteins, these are clubbed into what is called proteomics, which is also riding the wave of computational and electronic revolutions in biology. Using such omic technologies for agriculture however lags behind compared to their use in medicine. Nevertheless, the recent appreciation of the need for food and feed security under the predicted climatodemographic scenarios is pushing the frontiers of agricultural research. The use of proteomics in particular is gaining ground rather quickly, and an increased understanding of crop phenotype as related to proteomics is

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Section: Forage Crop Proteomicsmentioning

confidence: 99%

Translating the Genome for Translational Research: Proteomics in Agriculture

Caguioa

Raorane

Kohli

2015

Plant Biology and Biotechnology

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“…Subsequently, proteome responses to cold were studied in a much broader range of plants including Arabidopsis cold-and salt-tolerant relative Thellungiella salsuginea (Gao et al 2009), chicory (Degand et al 2009), Festuca pratensis (Kosmala et al 2009), soybean , pea (Dumont et al 2011), Lolium perenne (Bocian et al 2011), woody plants such as peach (Renaut et al 2008), and also wheat Sarhadi et al 2010;Rinalducci et al 2011a, b) (reviewed in Kosová et al 2011). Unfortunately, for barley limited information is available on proteome variation following exposure to low-temperature.…”

Section: Proteomicsmentioning

confidence: 99%

“…In contrast, changes in abundance of proteins involved in CO 2 assimilation (Calvin cycle), especially Rubisco large and small subunits and Rubisco activase proteins, are more complex and less clear. Increased abundance of several Calvin cycle components is in fact often observed (Gao et al 2009;Bocian et al 2011;Dumont et al 2011) since rate of enzymatically-catalyzed reactions generally decreases at low temperatures. However, cold acclimation processes reveal increased energy demands, therefore in cold conditions, the CO 2 assimilation needs to be efficiently regulated.…”

Section: Proteomicsmentioning

confidence: 99%

Genomics of Low-Temperature Tolerance for an Increased Sustainability of Wheat and Barley Production

Pecchioni

Kosová

Vítámvás

et al. 2013

Genomics of Plant Genetic Resources

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Stability of high yields in a changing environment becomes the main aim of the future wheat and barley breeding, oriented towards development of frosttolerant winter and facultative cultivars together with careful selection of growth cycle adaptation and drought tolerance. Since low temperature signal influences both the cold acclimation and vernalization processes the interaction between VRN gene expression and freezing tolerance (FT) is discussed. Recent advances in global expression changes driven by cold are reviewed in view of the immense progress in high throughput technological platforms. Different signal transduction pathways in which several transcription factors play an important role regulating the expression of whole sets of genes are presented, including CBF-regulated and CBF-independent hubs. The knowledge acquired from genomics and transcriptome analysis has been then complemented by the description of metabolomics and proteomic approaches to help unraveling the molecular changes that occur under cold stress in the cereal plants. Finally, it is surveyed the great importance of stable and well-characterized genetic resources for future breeding for FT, that could switch from marker-assisted to genomics-assisted selection.

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“…Procedures based on two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) have become one of the most powerful techniques of plant proteomics (Nordhoff et al 2001;Weiss and Görg 2007;Bocian et al 2011;Correia et al 2012). Combination relationships of protein populations, global protein identification along with internal patterns analysis, have ensured new insights into the connection of biochemical processes of different proteomes (Correia et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Proteomic signature of fenugreek treated by methyl jasmonate and cholesterol

et al. 2017

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Main conclusion Changes in proteome level as a result of methyl jasmonate and cholesterol treatment were investigated. The identified proteins were often involved in response to stress caused by various treatments. Furthermore, 18 proteins were expressed in treatmentspecific manner. In this study, the fenugreek plants were treated with methyl jasmonate (as an elicitor) and cholesterol (as a precursor of steroids and steroidal saponins) to check reaction at the level of the proteome to stress and to investigate steroidal saponin (diosgenin) biosynthesis. Proteins were separated by two-dimensional electrophoresis (2-DE) and identified by MALDI-ToF/ToF followed by database searches using Mascot search engine. Totally, 63 and 41 protein spots were differentially expressed after methyl jasmonate and cholesterol treatment, respectively. These proteins were classified into seven groups: photosynthesis, energy, metabolism, protein metabolism, secondary metabolism, stress and defense, and other. We found that 9 proteins were responsive to all treatments, and 18 proteins expressed in treatment-specific manner. Higher level of photosynthetic proteins sensitive to both biotic and abiotic stimuli was detected. In addition, proteins related to the stress (especially oxidative) and defense, protein, and secondary metabolism were overexpressed. The results indicate that methyl jasmonate and cholesterol elicited a defense reaction at the proteome level as a response to stress. The usefulness of 2-DE method for identification of proteins related with species-specific metabolic pathways is restricted. Integration of transcriptome data with proteomic analysis improved annotation process.

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Differences in leaf proteome response to cold acclimation between Lolium perenne plants with distinct levels of frost tolerance

Cited by 47 publications

References 28 publications

Translating the Genome for Translational Research: Proteomics in Agriculture

Translating the Genome for Translational Research: Proteomics in Agriculture

Genomics of Low-Temperature Tolerance for an Increased Sustainability of Wheat and Barley Production

Proteomic signature of fenugreek treated by methyl jasmonate and cholesterol

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