Identification of Fusarium Species Isolated From Stored Apple Fruit in Croatia / Identifikacija Vrsta Roda Fusarium Izoliranih S Plodova Jabuke Nakon Skladištenja

Sever, Zdravka; Ivić, Dario; Kos, Tomislav; Miličević, Tihomir

doi:10.2478/10004-1254-63-2012-2227

Cited by 32 publications

(9 citation statements)

References 11 publications

(10 reference statements)

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“…Rossman & Samuels, M. fructigena, Cladosporium spp., P. expansum, Phacidiopycnis washingtonensis Xiao & J.D.Rogers, C. acutatum, Gibberella avenacea R.J. Cook, B. cinerea were present up to 5% (Maxin et al, 2012a). The Fusarium rot has been detected on 9 to 30% of apples depending on cultivar stored in Ultra Low Oxygen (ULO) conditions in Croatia (Sever et al, 2012). Blue mold decay caused by P. expansum is damaging 30 to 60% of cold-stored apples in France, and it is important disease not only in other European countries but also in the USA (Morales et al, 2010).…”

Section: Introductionmentioning

confidence: 90%

“…Differences of cultivars in infection level have been described in other investigations, for instance, in the case of infections with Fusarium spp. (Sever et al, 2012).…”

Section: Fungi Causing Storage Rot Of Apple Fruit In Integrated Pest mentioning

confidence: 99%

“…Apple rot incidence may vary depending on cultivar (Sever et al, 2012); Weber, 2011) and harvest time (Borve, Roen, & Stensvand, 2013). Climate conditions during growing season can also have a significant impact, for example, on spore dispersal and following brown rot incidence as it is proved in the conidial dispersal rates in the case of M. fructigena in organic and integrated apple orchards in Hungary (Holb, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Fungi Causing Storage Rot of Apple Fruit in Integrated Pest Management System and their Sensitivity to Fungicides

Grantina-Ievina

2015

Rural Sustainability Research

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Abstract. Apple fruit rot can be caused by several fungi. In Northern Europe, the most common storage rot, Bull's eye rot, is caused by Neofabraea spp., bitter rot by Colletotrichum spp., brown rot by Monilinia fructigena, grey mould is caused by Botrytis cinerea and Fusarium rot by several Fusarium species. Blue mold decay caused by Penicillium expansum is an important disease in several European countries. Incidence of different causal agents may vary depending on cultivar, climate during growing season and agricultural practices. The main objective of the study was to obtain baseline information about apple rot-causing fungi, their incidence during fruit storage and to evaluate the fungicide sensitivity of most of isolated fungal species. The study was performed during the storage period of apples after the growth season of 2013. Rotten apples were sorted in the storage and part of them was brought to the laboratory in order to obtain fungal isolates. Fungi were identified according to the morphological characteristics and sequencing of the ITS1-5.8S-ITS2 region. During storage in February and March the total percentage of rotten apples in various cultivars varied from 3.6 to 58.9%. All post-harvest diseases described in Northern Europe were detected. In part of the storehouses apple rot caused by Cadophora luteo-olivacea was observed. Alternaria spp. and Cladosporium spp. were detected on few apples as secondary infection agents. Using the most often isolated fungal species, sensitivity tests were performed against five commonly used fungicides. In general, the sensitivity of tested fungi to the fungicides was high with exception of several Neofabraea and Alternaria isolates.

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Section: Introductionmentioning

confidence: 90%

“…Differences of cultivars in infection level have been described in other investigations, for instance, in the case of infections with Fusarium spp. (Sever et al, 2012).…”

Section: Fungi Causing Storage Rot Of Apple Fruit In Integrated Pest mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fungi Causing Storage Rot of Apple Fruit in Integrated Pest Management System and their Sensitivity to Fungicides

Grantina-Ievina

2015

Rural Sustainability Research

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“…[ 27–29 ] The toxigenic potential of many Fusarium species emphasizes the need for accurate identification on the species level. [ 29 ] Fusarium species can be identified based on morphology alone. [ 12 ] However, identification based on molecular data is considered more reliable and accurate than morphological identification and has become much more important in diagnostics of the fungi from the genus Fusarium .…”

Section: Resultsmentioning

confidence: 99%

Toxigenic profiles and trinucleotide repeat diversity ofFusariumspecies isolated from banana fruits

Alghuthaymi

Bahkali

2015

Biotechnology & Biotechnological Equipment

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Infesting Fusarium species isolated from banana fruit samples were identified and quantified by morphological, mycotoxicological and molecular tools. A total of 19 Fusarium isolates were obtained: F. semitectum was most predominant (26%), followed by F. proliferatum (16%), F. circinatum (16%), F. chlamydosporum (10.5%), F. solani (10.5%), F. oxysporum (10.5%) and F. thapsinum (5%). Fumonisin B1, deoxynivalenol and zearalenone contents were assayed by high-performance liquid chromatography (HPLC). Seventeen isolates, belonging to F. chlamydosporum, F. circinatum, F. semitectum, F. solani, F. thapsinum, F. proliferatum and Fusarium spp., produced mycotoxins when cultured on rice medium. Fumonisin was produced by all of the studied Fusarium isolates, except F. oxysporum, at a concentration of over 1 μg/mL. F. citrinium isolates 4 and 5 and F. solani isolate 3 were the most potent producers of deoxynivalenol. We compared the 19 Fusarium isolates based on the bands amplified by 10 microsatellite primers. Of these, seven primers, (TCC)5, (TGG)5, (GTA)5, (ATG)5, (TAC)5, (TGC)5 and (TGT)5, yielded a high number of bands and different mean number of alleles. The similarity level between isolates was calculated using a simple matching coefficient. Dendrograms were constructed by the unweighted pair-group method with arithmetical averages (UPGMA). Two main clusters were observed. The interspecific genetic similarity between Fusarium spp. isolates was between 40% and 58% and the intraspecific similarity from 58% to 100%, indicating a high degree of genetic diversity in the tested isolates. Some unexpected genetic similarities were observed among the isolates, indicating non-agreement between morphological and molecular identification of the isolates.

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“…The most common causal agents of decay of stored apple and pear fruits in the genus Fusarium are: F. avenaceum, F. culmorum, F. lateritium, F. solani, as well as F. pseudograminearum, F. semitectum, F. crookwellense, F. proliferatum and F. compactum (Konstantinou et al, 2011;Sever et al, 2012;Kou et al, 2014;Wenneker et al, 2016). According to Sørensen et al (2009), F. avenaceum produce high amounts of mycotoxins in naturally and artificially inoculated apple fruits, and therefore, monitoring of its occurrence on stored apples is of high importance.…”

Section: Introductionmentioning

confidence: 99%

Effects of 1-MCP and dynamic controlled atmosphere on apple fruit rot caused by Fusarium avenaceum

et al. 2018

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Fusarium species are increasingly detected as the causal agents of decay of stored apple fruits. Fusarium avenaceum is particularly significant due to its predominant occurrence among Fusarium species in stored apple fruits and its ability to produce mycotoxins. Treatments with 1-methylcyclopropene (1-MCP) and different storage conditions affect the aggressiveness of F. avenaceum and development of fungal-caused decay in stored apple fruits. In this study, apple fruits (cv. 'Granny Smith') were treated with 1-MCP, and artificially inoculated with F. avenaceum. The isolate used for inoculation, originating from apple fruit, was identified based on morphological characteristics and by polymerase chain reaction (PCR) using a species-specific primer pair (FA-ITSF and FA-ITSR) for F. avenaceum. After inoculation, treated and untreated fruits were stored at room temperature and cold-stored under dynamic controlled atmosphere (DCA). Diameters of necrotic lesions were measured after 7, 14 and 21 days of incubation on fruits stored at room temperature, while necrosis diameters on DCA-stored fruits were measured immediately at the end of storage period (143 days), and after 7, 14 and 21 days of additional incubation at room temperature. The results show that treatment with 1-MCP inhibits the development of F. avenaceum on apple fruits during storage under DCA. However, after storage, i.e. during incubation at room temperature, no significant difference between 1-MCP-treated and untreated fruits was observed. On fruits stored at room temperature only, no difference between 1-MCP-treated and untreated fruits was observed. However, 1-MCP-treated fruits stored at room temperature only developed significantly smaller necrosis lesions compared to 1-MCP-treated and DCA stored fruits. It infers that both 1-MCP treatment and DCA storage inhibit fungal decay caused by F. avenaceum on apple fruits. However, the effects do not persist after storage.

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Identification of Fusarium Species Isolated From Stored Apple Fruit in Croatia / Identifikacija Vrsta Roda Fusarium Izoliranih S Plodova Jabuke Nakon Skladištenja

Cited by 32 publications

References 11 publications

Fungi Causing Storage Rot of Apple Fruit in Integrated Pest Management System and their Sensitivity to Fungicides

Fungi Causing Storage Rot of Apple Fruit in Integrated Pest Management System and their Sensitivity to Fungicides

Toxigenic profiles and trinucleotide repeat diversity ofFusariumspecies isolated from banana fruits

Effects of 1-MCP and dynamic controlled atmosphere on apple fruit rot caused by Fusarium avenaceum

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