Identification of Fur in Pasteurella multocida and the Potential of Its Mutant as an Attenuated Live Vaccine

Abstract

Pasteurella multocida is a pathogenic microorganism that causes a variety of serious diseases in humans and animals worldwide. The global regulator gene, fur, plays an important role in pathogenesis and regulates the virulence of many bacteria. Here, we identified a fur gene in P. multocida by complementing a Salmonella Choleraesuis Δfur mutant, and characterized a fur mutant strain of P. multocida. The P. multocida Δfur mutant strain exhibited no significant differences in growth and outer membrane protein (OMP) profiles when the complemented strain was compared to the parent. Ducks were used as the model organism to determine the virulence and protection efficacy induced by Δfur mutant strain. Animal experiments showed that colonization by the mutant was decreased by oral infection of live Δfur mutant strain. The LD50 of the ducks infected with the Δfur mutant was 146-fold higher than that of the ducks infected with the wild-type strain when administered through the oral route. Evaluation of the immunogenicity and protective efficacy of the Δfur mutant of P. multocida revealed strong serum IgY and bile IgA immune responses following oral inoculation with the Δfur strain. Ducks that were orally inoculated with the Δfur mutant strain demonstrated 62% protection efficacy against severe lethal challenge with the wild-type P. multocida. This study provides new insights into P. multocida virulence and the potential use of an attenuated vaccine against P. multocida.

First report from Bangladesh on genetic diversity of multidrug-resistant Pasteurella multocida type B:2 in fowl cholera

“…The route of infection is oral or nasal with transmission through nasal exudate, faces, contaminated soil, equipment, and people [7]. Different serogroups of P. multocida have been identified as the etiologic agent for many infectious diseases in a wide spectrum of hosts, including poultry and wild birds (FC), pigs (rhinitis and pneumonia), cattle, buffaloes, and small ruminants (hemorrhagic septicemia [HS] and enzootic pneumonia,) rabbits (snuffles), cats, dogs, and other mammals (upper respiratory tract infections and cellulitis) [8][9][10][11].…”

“…P. multocida is a zoonotic Gram-negative and opportunistic bacterium [12], and the strains of P. multocida are divided into five serotypes such as A (hyaD-hyaC), B (bcbD), D (dcbF), E (ecbJ), and F (fcbD based on capsular typing [9,10]. Among these serotypes, capsular serogroups A and F cause the majority of FC, whereas serogroups B and E are predominantly associated with HS in cattle and wild ruminants [8,13].…”

“…In addition to capsular serogroups, P. multocida strains are currently classified into 16 Heddleston lipopolysaccharides (LPS) serovars [14,15]. Different serotypes of the P. multocida exhibit varying degrees of virulence in different hosts [9,10]. Various strains of P. multocida produce a wide arsenal of virulence factors that are crucial for pathogenesis.…”

Stimulus Control of Odorant Concentration: Pilot Study of Generalization and Discrimination of Odor Concentration in Canines

“…However, assuming a vapor pressure of ~6 mmHg (PubChem, Isoamyl acetate, CID = 31,276), the anticipated vapor concentration (assuming ideal conditions for Raoult’s law) would be approximately 90 parts-per-billion for the 10-fold dilution of the target odorant. Given that recent work has shown amyl acetate thresholds to be close to 1 parts-per-trillion for dogs [ 30 , 31 ], our test concentrations should be well above the dog detection limits. This suggests that the low probability of response was a generalization failure, not a sensitivity failure.…”

Transcriptomic Analysis of Chicken Lungs Infected With Avian and Bovine Pasteurella multocida Serotype A

“…In the case of peracute or acute infection, the lung lesions dominated by hemorrhages can be characteristic of fowl cholera (6). Antibiotics are still the main treatment for pasteurellosis due to the lack of effective multi-serotype vaccines (7). In some cases, P. multocida can cause human infection via animal bites and scratches (8,9).…”