Transcriptomic Analysis of Chicken Lungs Infected With Avian and Bovine Pasteurella multocida Serotype A

Li, Pan; He, Fang; Wu, Chenlu; Zhao, Guangfu; Hardwidge, Philip R.; Li, Nengzhang; Peng, Yuanyi

doi:10.3389/fvets.2020.00452

Cited by 9 publications

(12 citation statements)

References 61 publications

(73 reference statements)

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“…The study on genomic analysis of a highly virulent P. multocida strain PmCQ2 induced host response suggested that PmCQ2 infection may activate NOD-like receptor signaling pathway ( Wu et al, 2017 ; Li et al, 2020 ; He et al, 2021 ). The central protein of the inflammasome complex include NOD-like receptor (NLR) family, AIM2-like receptor (ALR) family, and pyrin protein.…”

Section: Introductionmentioning

confidence: 99%

The Critical Role of Potassium Efflux and Nek7 in Pasteurella multocida-Induced NLRP3 Inflammasome Activation

et al. 2022

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Pasteurella multocida is a zoonotic pathogen causing respiratory infection in different animal species such as cattle, sheep, pigs, chickens and humans. Inflammasome is a complex assembled by multiple proteins in the cytoplasm and plays an important role in the host defense against microbial infection. Bovine Pasteurella multocida type A (PmCQ2) infection induces NLRP3 inflammasome activation and IL-1β secretion, but the mechanism of PmCQ2-induced activation of NLRP3 inflammasome is still unknown. Therefore, the underlying mechanism was investigated in this study. The results showed that potassium efflux mediated PmCQ2-induced IL-1β secretion and blocking potassium efflux attenuated PmCQ2-induced caspase-1 activation and ASC oligomerization. Furthermore, NIMA-related kinase 7 (Nek7) was also involved in PmCQ2-induced caspase-1 activation and IL-1β secretion. In addition, PmCQ2 infection promoted Nek7-NLRP3 interaction, which is dependent on potassium efflux. In conclusion, our results indicate the critical role of potassium efflux and Nek7 in Pasteurella multocida-induced NLRP3 inflammasome activation, which provides useful information about Pasteurella multocida-induced host immune response.

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Section: Introductionmentioning

confidence: 99%

The Critical Role of Potassium Efflux and Nek7 in Pasteurella multocida-Induced NLRP3 Inflammasome Activation

et al. 2022

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“…Additionally, various papers have demonstrated that AA has an impact on macrophage function, and macrophages played a vital role during P. multocida infection (48). For instance, intracellular accumulation of pharmacologic AA concentrations in human monocytes inhibited its apoptosis pathway activation (49).…”

Section: Discussionmentioning

confidence: 99%

“…As shown in Figure 4A , supplementation with AA significantly reduced the lung bacterial density while supplementation with Asp only has an upward trend on lung bacterial density. Additionally, various papers have demonstrated that AA has an impact on macrophage function, and macrophages played a vital role during P. multocida infection ( 48 ). For instance, intracellular accumulation of pharmacologic AA concentrations in human monocytes inhibited its apoptosis pathway activation ( 49 ).…”

Section: Discussionmentioning

confidence: 99%

L-Ascorbic Acid Shapes Bovine Pasteurella multocida Serogroup A Infection

Zhao

et al. 2021

Front. Vet. Sci.

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Bovine Pasteurella multocida serogroup A (bovine PmA) is one of the most important pathogens causing fatal pneumonia in cattle. However, it is largely unknown how nutrition shapes bovine PmA infection. Here, we discovered that the infected lung held the highest bacterial density than other tissues during infection. By screening the different metabolites between high (lung)- and low (liver)-bacterial density tissues, the present work revealed that L-ascorbic acid and L-aspartic acid directly influenced bovine P. multocida growth. Interestingly, L-ascorbic acid, which is expressed at higher levels in the infected livers, inhibited bovine PmA growth as well as virulence factor expression and promoted macrophage bactericidal activity in vitro. In addition, ascorbic acid synthesis was repressed upon bovine PmA infection, and supplementation with exogenous L-ascorbic acid significantly reduced the bacterial burden of the infected lungs and mouse mortality. Collectively, our study has profiled the metabolite difference of the murine lung and liver during bovine PmA infection. The screened L-ascorbic acid showed repression of bovine PmA growth and virulence expression in vitro and supplementation could significantly increase the survival rate of mice and reduce the bacterial load in vivo, which implied that L-ascorbic acid could serve as a potential protective agent for bovine PmA infection in clinic.

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“…Interestingly, several of these DEGs [e.g., interleukin-1beta (IL-1β), interleukin 4-induced 1 (IL4I1), interleukin 1 receptor type 2 (IL1R2), IL-22, and suppressor of cytokine signaling 3 (SOCS3) cytokines] showed the same expression trend and were upregulated in P. multocida -infected lungs of goats and those of chicken. Through an analysis of the enriched KEGG pathways, we found that many of these same DEGs are involved in the p53 signaling pathway and cytokine–cytokine receptor interactions ( 33 ). The most enriched KEGG pathway cytokine–cytokine receptor interaction was also enriched by the mouse lung DGEs that infected with P. multocida ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Differential Expression Profiling of Pulmonary circRNAs Associated With Immune Reaction to Pasteurella multocida in Goats

et al. 2021

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Pasteurella multocida is a highly versatile pathogen that infects a wide range of animals, including goats, causing pneumonia and hemorrhagic septicemia. Circular RNA (circRNA) is a type of non-coding RNA that plays an important role in regulating cellular metabolism. However, whether and how circRNA is involved in regulating immune responses in the goat lung has not been reported. Thus, this study was designed to examine the function of circRNA in goats infected with Pasteurella multocida. Goats were assigned into one of two groups: an uninfected control group (CK) and an infected group challenged with P. multocida. Compared with the CK group, which remained healthy, the infected goats showed clinical signs of infection, including depression, cough, nasal discharge, and dyspnea, along with elevated body temperature and lesions in the lung. Whole-transcriptome sequencing and small RNA sequencing were then performed using lung samples from goats from each group. A total of 138 circRNA, 56 microRNAs (miRNA), and 2,673 messenger RNA (mRNA) molecules were significantly differentially expressed in the P. multocida-infected group compared with the CK group. Randomly selected differentially expressed circRNA, miRNA, and mRNA molecules (n = 5 per group) were then validated by quantitative reverse-transcriptase polymerase chain reaction analysis. Gene ontology (GO) analysis of the source genes indicated that six immune-related terms were enriched among the differentially expressed cirRNA molecules, including inflammatory response, immune effector process, cell activation involved in immune response, cytokine-mediated signaling pathway, response to endogenous stimulus, and immune response. The corresponding circRNA molecules were then selected for construction of a competitive endogenous RNA network to identify networks that may be involved in the immune response to P. multocida infection. The results indicated that P. multocida HN01 may cause pneumonia and stimulate an immune response in goats via regulation of circRNA expression. This study presents the first comprehensive circRNA profile in response to P. multocida infection in goats, thus, providing a basis for understanding the function of circRNA in the host immune response to P. multocida infection.

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Transcriptomic Analysis of Chicken Lungs Infected With Avian and Bovine Pasteurella multocida Serotype A

Cited by 9 publications

References 61 publications

The Critical Role of Potassium Efflux and Nek7 in Pasteurella multocida-Induced NLRP3 Inflammasome Activation

The Critical Role of Potassium Efflux and Nek7 in Pasteurella multocida-Induced NLRP3 Inflammasome Activation

L-Ascorbic Acid Shapes Bovine Pasteurella multocida Serogroup A Infection

Genome-Wide Differential Expression Profiling of Pulmonary circRNAs Associated With Immune Reaction to Pasteurella multocida in Goats

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