Identification of Food-Derived Elastin Peptide, Prolyl-Glycine (Pro-Gly), in Human Blood after Ingestion of Elastin Hydrolysate

Abstract

Elastin hydrolysate has apparent beneficial effects, and the food-derived peptide prolyl-glycine (Pro-Gly) is present in human blood after oral ingestion. Following ingestion of elastin hydrolysate (10 g/60 kg body weight) by healthy human volunteers, peripheral blood was used to prepare plasma samples from which peptides were extracted by solid phase extraction and fractionated by size-exclusion chromatography (SEC). Peptides in the SEC fractions were derivatized with phenyl isothiocyanate (PITC) and resolved by reversed phase (RP)-HPLC. Pro-Gly was the major food-derived elastin peptide, reaching a maximum (18 μM) at 30 min after ingestion, and decreasing to approximately 20% at 4 h after ingestion. Finally, in cell culture, levels of Pro-Gly in the medium above 0.1 μg/mL significantly enhanced elastin synthesis of normal human dermal fibroblasts (NHDF) without affecting the rate of cell proliferation.

Elastin hydrolysate derived from fish enhances proliferation of human skin fibroblasts and elastin synthesis in human skin fibroblasts and improves the skin conditions

“…Elastin synthesis was enhanced depending on concentrations of elastin hydrolysate and Pro‐Gly. These results are in agreement with the literature …”

“…Based on these parameters, concentration of Pro‐Gly in human blood is an important factor. We estimated that Pro‐Gly was detected at approximately 0.14 µmol L −1 (24 ng mL −1 ) after ingestion of 75 mg of elastin hydrolysate, based on a previous study where Pro‐Gly was detected at approximately 18 µmol L −1 30 min after ingestion of 10 g of elastin hydrolysate . Pro‐Gly at 0.14 µmol L −1 would have effects on cell proliferation and elastin synthesis according to results from the present study.…”

“…In this study, we investigated effects of elastin hydrolysate and Pro‐Gly on human skin fibroblasts and skin condition. In a previous study, 0, 100, 1000 and 10 000 ng mL −1 of Pro‐Gly did not affect proliferation of NHDF cells . Based on these results, we examined lower concentrations of Pro‐Gly.…”

“…Interestingly, there seems to be a similar association between elastin and the prolyl‐glycine (Pro‐Gly) dipeptide. Pro‐Gly was detected in peripheral blood of human following oral ingestion of elastin hydrolysate derived from fish (skipjack) . Additionally, elastin synthesis by the human skin fibroblasts in vitro was significantly enhanced from 0.1 to 10 µg mL −1 Pro‐Gly after a 72‐h incubation period .…”

“…Pro‐Gly was detected in peripheral blood of human following oral ingestion of elastin hydrolysate derived from fish (skipjack) . Additionally, elastin synthesis by the human skin fibroblasts in vitro was significantly enhanced from 0.1 to 10 µg mL −1 Pro‐Gly after a 72‐h incubation period . While clinical trials with humans have not yet been conducted, this result suggests that oral ingestion of elastin hydrolysate may have the potential to improve human skin conditions.…”