Identification of flavonoids of Rhodiola rosea by liquid chromatography-tandem mass spectrometry

Petsalo, Aleksanteri; Jalonen, Jorma; Tolonen, Ari

doi:10.1016/j.chroma.2005.11.056

Cited by 100 publications

(78 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[16 -18] The combination of liquid chromatography with ESI mass spectrometry (LC/ESI-MS) demonstrates another advantage of using MS, as it provides the molecular weight and the structural information of flavonoid glycosides present in mixtures. [19] A study on structural characterization of flavonoids revealed that the position of sugar substitution has a significant influence on the fragmentation pattern of flavonoid O-glycosides. [20 -24] Previous research revealed that the formation of the aglycone ion (Y 0 − ) and the radical aglycone ion ([Y 0 − H] −• ) plays a significant role in the structural elucidation and differentiation of flavonoid O-glycoside isomers.…”

Section: Introductionmentioning

confidence: 99%

A study of characteristic fragmentation of isoflavonoids by using negative ion ESI‐MSⁿ

Ablajan

2010

J. Mass Spectrom.

View full text Add to dashboard Cite

Isoflavone mono-O-glycosides were investigated by electrospray ionization tandem mass spectrometry with a quadrupole linear ion trap mass spectrometer in negative ion mode. Isoflavonoids having different positions of glycosylation or methylation were differentiated according to the relative abundances of Y(0)(-) and [Y(0)-H](-•) ions generated from the [M-H](-) ion. It is found that the site of glycosyl or methyl group significantly affects relative abundances of the Y(0)(-) and [Y(0)-H](-•) ions. In addition, the characteristic ion [Y(0)-2H](-) was observed in the product ion spectrum of genistein 7-O-β-D-glucoside and was also detected, together with the [Y(0)-CH(3)](-•) and [Y(0)-H-CH(3)](-) ions in the product ion spectra of glycitin and 6-methoxy genistein 7-O-β-D-glucoside. The structures of isoflavonoids can be characterized and identified according to the formation of these diagnostic ions. The results obtained from this investigation can promote the rapid identification of isoflavonoids in crude plant extracts.

show abstract

Section: Introductionmentioning

confidence: 99%

A study of characteristic fragmentation of isoflavonoids by using negative ion ESI‐MSⁿ

Ablajan

2010

J. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…The most important biologically active constituents are phenolics [14]. There are mainly: phenylpropanoids (rosavin, rosin and rosarin), phenyletanoids (salidroside and p-tyrosol), flavonoids, proanthocyanidins as well as tannins [15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Biometric and phytochemical variability of roseroot (Rhodiola rosea L.) from field cultivation

2014

View full text Add to dashboard Cite

S u m m a r y Roseroot (Rhodiola rosea L.) is an adaptogenic plant, widely used in the traditional medicine of Scandinavia, Russia, China and Mongolia. The aim of the study was to determine the biometric and phytochemical co-variability of this species under field cultivation in western Poland (Plewiska near Poznań). The plant material originated from four-year-old cultivation established twice by rhizome division in autumn 2007 and 2008. In the study, 46 individuals (23 plants in 2011 and in 2012) were used. The biometric analysis included measurements of the basic characteristics of plants related to the size (luxuriance) of clumps, shoots, leaves and rhizomes with roots (raw material). The amounts of total polyphenols (expressed as gallic acid), tannins (expressed as pyrogallol) and flavonoids (expressed as quercetin) were determined spectrophotometrically. The obtained results showed high variation of Rh . rosea, especially in the level of flavonoids (0.01-0.20% DM) and in the weight of raw material (113-1156 g FM/plant). There were observed correlations between the phytochemical (flavonoid and total phenolic content) and biometric (water content, leaf number, shoot and clump size) features.

show abstract

“…Peak 4 was identified as avicularin (Lhuillier et al, 2007). Peaks 11 and 12 were identified as quercetin and kaempferol (Hvattum and Ekeberg 2003;Petsalo et al, 2006), respectively. All compounds mentioned above were identified by analysis the UV, MS/MS, as well as 1 H NMR data; the data were shown in Table 1.…”

Section: Resultsmentioning

confidence: 99%

Fast differentiation of Apocynum venetum with related species by UPLC/MS and UPLC/SPE/NMR

et al. 2011

View full text Add to dashboard Cite

The newly established hyphenated instrumentation of UPLC/MS and UPLC/SPE/NMR techniques has been applied for a fast comparison of the major chemical components from Chinese medicinal herb Apocynum venetum and related species. The content of the compounds among three species was found to be highly variable qualitatively as well as quantitatively. The identification of the constituents was based on matching their UV spectrum, online UPLC/MS/MS, and UPLC/SPE/NMR data. The system successfully identified 16 compounds in one step via operation for 4 h. Hyperforin was described for the first time in A. venetum, all of which had not previously been reported in Apocynum hendersonii and Apocynum pictum.

show abstract

Identification of flavonoids of Rhodiola rosea by liquid chromatography-tandem mass spectrometry

Cited by 100 publications

References 21 publications

A study of characteristic fragmentation of isoflavonoids by using negative ion ESI‐MSⁿ

A study of characteristic fragmentation of isoflavonoids by using negative ion ESI‐MSⁿ

Biometric and phytochemical variability of roseroot (Rhodiola rosea L.) from field cultivation

Fast differentiation of Apocynum venetum with related species by UPLC/MS and UPLC/SPE/NMR

Contact Info

Product

Resources

About

Identification of flavonoids of Rhodiola rosea by liquid chromatography-tandem mass spectrometry

Cited by 100 publications

References 21 publications

A study of characteristic fragmentation of isoflavonoids by using negative ion ESI‐MSn

A study of characteristic fragmentation of isoflavonoids by using negative ion ESI‐MSn

Biometric and phytochemical variability of roseroot (Rhodiola rosea L.) from field cultivation

Fast differentiation of Apocynum venetum with related species by UPLC/MS and UPLC/SPE/NMR

Contact Info

Product

Resources

About

A study of characteristic fragmentation of isoflavonoids by using negative ion ESI‐MSⁿ

A study of characteristic fragmentation of isoflavonoids by using negative ion ESI‐MSⁿ