Fast differentiation of Apocynum venetum with related species by UPLC/MS and UPLC/SPE/NMR

Abstract

The newly established hyphenated instrumentation of UPLC/MS and UPLC/SPE/NMR techniques has been applied for a fast comparison of the major chemical components from Chinese medicinal herb Apocynum venetum and related species. The content of the compounds among three species was found to be highly variable qualitatively as well as quantitatively. The identification of the constituents was based on matching their UV spectrum, online UPLC/MS/MS, and UPLC/SPE/NMR data. The system successfully identified 16 compounds in one step via operation for 4 h. Hyperforin was described for the first time in A. venetum, all of which had not previously been reported in Apocynum hendersonii and Apocynum pictum.

Quality Evaluation of Apocyni Veneti Folium from Different Habitats and Commercial Herbs Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis

“…At present, due to the shortage of AVF, the leaves of Poacynum hendersonii (Hook. f.) Woodson (PHF), a non-official plant species, have been used indiscriminately as a substitute and medicinal resource for many years regardless of their potential interspecific differences in components [ 30 ]. Adulteration with PHF seriously affects the safety and efficacy of AVF.…”