Identification of fatty acid steryl esters in margarine and corn using direct flow injection ESI-MSn ion trap-mass spectrometry

Hailat, Iyad; Helleur, Robert

doi:10.1016/j.ijms.2014.02.017

Cited by 10 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S2C) was similar to stigmasteryl glycoside, but the main ion m / z 69 suggested a methyl group at C24. In addition, its mass spectrum obtained in this study shows some similarities to its atmospheric‐pressure chemical ionization MS‐CID spectrum in a previous report …”

Section: Resultssupporting

confidence: 79%

See 1 more Smart Citation

Characterization of steryl glycosides in marine microalgae by gas chromatography–triple quadrupole mass spectrometry (GC–QQQ‐MS)

Zhang

Ran

et al. 2017

J Sci Food Agric

View full text Add to dashboard Cite

show abstract

Section: Resultssupporting

confidence: 79%

“…C) exhibit similar fragment patterns. Those similar ions m / z 147, 161, 135, 189, 121, 95, and 81 were produced by multi‐cleavages of carbon rings suggested by Hailat and Helleur . In particular, fragment ions m / z 147 and 161 were assigned as steryl cations having a double bond in the B ring.…”

Section: Resultsmentioning

confidence: 87%

Characterization of steryl glycosides in marine microalgae by gas chromatography–triple quadrupole mass spectrometry (GC–QQQ‐MS)

Zhang

Ran

et al. 2017

J Sci Food Agric

View full text Add to dashboard Cite

show abstract

“…Structural confirmation of steryl ester isomers was successfully accomplished by performing second generation MS analysis, i.e.MS 3 . In fact ESI was superior in analyzing intact sterol esters compared to APCI that predominantly formed [M+H-HOOCR] + ions resulting from in-source fragmentation 95 .…”

Section: Liquid Chromatography-electrospray Ionization-mass Spectromementioning

confidence: 99%

Mass Spectrometric Approaches for the Analysis of Phytosterols in Biological Samples

Gachumi

El‐Aneed

2017

J. Agric. Food Chem.

View full text Add to dashboard Cite

Plant sterols (phytosterols) are important structural components of plant cellular membranes, and they play a major role during development and metabolism. They have health-associated benefits, especially in lowering blood cholesterol levels. Because of their many health claims, there is a growing interest in their analysis. Although various analytical strategies have been employed in analyzing phytosterols, chromatography linked to mass spectrometry (MS) is superior due to its sensitivity. Furthermore, specificity and selectivity are enhanced by utilizing tandem mass spectrometry (MS/MS). This article reviews the various mass spectrometric strategies used for the analysis of phytosterols. It highlights the applications and limitations associated with each MS strategy in various sample matrixes such as plant, human, animal, food, and dietary supplements. GC-MS was historically the method of choice for analysis; however, the derivatization step rendered it tedious and time-consuming. On the other hand, liquid chromatography coupled to MS (LC-MS) simplifies the analysis. Many ionization techniques have been used, namely, electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photoionization (APPI). APCI showed superiority in terms of ion intensity and consistency in ion formation, primarily forming [M + H - HO] ions rather than [M + H]. In addition, matrix assisted laser desorption ionization (MALDI) as well as ambient mass spectrometry such as direct analysis in real time (DART) have also been evaluated.

show abstract

“…ammonium salts, are frequently applied for the analysis of less polar compounds via ESI. 10 Moreover the usage of 2-(2-methoxyethoxy)ethanol (2-MEE) as a postcolumn additive for liquid chromatography coupled to negative mode ESI-MS proved to be beneficial. 11,12 In this study, we present the utilization of so-called proton sponges for the analysis of polar acidic compounds with ESI-MS. Proton sponges are a special class of aromatic diamines that possess a very high proton affinity and due to their extremely high pKa-value they tend to deprotonate acidic analyte molecules.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Using aromatic polyamines with high proton affinity as “proton sponge” dopants for electrospray ionisation mass spectrometry

Wirth

Rüger

Sklorz

et al. 2017

Eur J Mass Spectrom (Chichester)

View full text Add to dashboard Cite

Proton sponges are polyamines with high proton affinity that enable gentle deprotonation of even mildly acidic compounds. In this study, the concept of proton sponges as signal enhancing dopants for electrospray ionisation is presented for the first time. 1,8-Bis(dimethylamino)naphthalene (DMAN) and 1,8-bis(tetramethylguanidino)naphthalene (TMGN) were chosen as dopants, using methanol and acetonitrile/methanol as solvents. Individual standard compounds, compound mixtures and a diesel fuel as a complex sample matrix were investigated. Both proton sponges enhanced signal intensities in electrospray ionisation negative mode, but TMGN decomposed rapidly in methanolic solution. Significantly higher signals were only achieved using the acetonitrile/methanol mixture. On average a more than 10-fold higher signal intensity was measured with 10mol l DMAN concentration. A stronger signal increase of alcohol functionalities was observed compared to acid functionalities. All compound classes which were detected in the diesel fuel (CH- and CHO-class) received roughly 100-fold higher signal intensities when using DMAN as a dopant. Furthermore, the number of detected compounds as well as the double bond equivalent of the detected compounds increased. The compound class distribution shifted when adding DMAN and the formerly dominant CHO-, CHO-, and CHO- classes received similar relative intensities as formerly less accessible classes. The findings depict DMAN as a promising additive for electrospray ionisation negative analysis of at least mildly acidic compounds, even within complex sample material.

show abstract

Identification of fatty acid steryl esters in margarine and corn using direct flow injection ESI-MSn ion trap-mass spectrometry

Cited by 10 publications

References 20 publications

Characterization of steryl glycosides in marine microalgae by gas chromatography–triple quadrupole mass spectrometry (GC–QQQ‐MS)

Characterization of steryl glycosides in marine microalgae by gas chromatography–triple quadrupole mass spectrometry (GC–QQQ‐MS)

Mass Spectrometric Approaches for the Analysis of Phytosterols in Biological Samples

Using aromatic polyamines with high proton affinity as “proton sponge” dopants for electrospray ionisation mass spectrometry

Contact Info

Product

Resources

About