Identification of F1 Cassava (<i>Manihot esculenta</i> Crantz) Progeny Using Microsatellite Markers and Capillary Electrophoresis

Abstract

Generation of genetic diversity is necessary in improving on the potential of cassava when faced with various biotic and abiotic challenges. Presently, cassava breeders are breeding for a number of traits, such as drought tolerance, early root bulking, yield, starch, beta-carotene, protein, dry matter, pest and disease resistance, by relying on genetic diversity that exists in manihot esculenta germplasm. Controlled pollination is one of the main methods used to generate genetic diversity in cassava. However, the process of controlled pollination especially in an open field is prone to contamination by illegitimate pollen right from the time of pollination, seed collection, nursery bed establishment to planting of the trials. Therefore, authentication of the progeny obtained from cassava crosses is very important for genetic studies. Twelve informative microsatellite markers were used to verify the authenticity of 364 F 1 progeny thought to come from four controlled parental crosses. The transmission of each allele at nine microsatellite loci was tracked from parents to progeny in each of the four Namikonga-derived F 1 cassava families. Out of the 364 F 1 progeny, 317 (87.1%) were true-to-type, 44 (12.1%) were a product of self-pollination and 3 (0.8%) were a product of open pollination. The consistency of the results obtained using microsatellite markers makes this technique a reliable tool for assessing the purity of progeny generated from cassava crosses.

Molecular Fingerprinting and Hybridity Authentication in Cowpea Using Single Nucleotide Polymorphism Based Kompetitive Allele-Specific PCR Assay

“…A final focus was made on the molecular assessment of hybridity of the F 1 plants derived from multi-parental crosses. Molecular determination of hybridity has been successfully demonstrated in various crops; tomato (Osei et al, 2020), maize (Salgado et al, 2006), lettuce (Patella et al, 2019a), Capsicum (Mongkolporn et al, 2004), rice (Yashitola et al, 2002), cotton (Dongre and Parkhi, 2005), cassava (Vincent et al, 2014), and peanut (Gomez et al, 2008). Apart from that on tomato, these studies deployed microsatellite markers for hybridity testing as a QC to determine true F 1 for advancement.…”

Paternity Assignment in White Guinea Yam (Dioscorea Rotundata) Half-Sib Progenies from Polycross Mating Design Using SNP Markers

“…Parental reconstruction has also been successfully done in other root and tuber crops such as cassava (Manihot esculenta Crantz) [40][41][42], potato (Solanum tuberosum L.) [43,44] and sweetpotato (Ipomoea batatas L.) [45]. In these crops, molecular parentage analysis facilitated the discovery of pedigree errors or mismatches in populations improved using natural and artificial mating schemes [44,46,47].…”

Confirmation of Alleles Inheritance in F1 Progenies Derived from a Cross of Calcutta-4 [Musa acuminata ssp. burmannicoides] and Musa acuminata ssp. microcarpa Based on SSR Markers

“…Berdasarkan pola pita polimorfik yang dihasilkan pada kedua tetua terlihat bahwa enam marka SSR polimorfik mampu menganalisis keragaman genetik pisang sebagaimana hasil penelitian sebelumnya (Li et al 2010;Changadeya et al 2012;Rotchanapreeda et al 2016;Nyine et al 2017). Sementara itu, penggunaan marka SSR untuk seleksi populasi persilangan telah banyak diaplikasikan pada beberapa tanaman, seperti kedelai (Santana et al 2014;Lestari et al 2017), jagung (Elci dan Hancer 2015), singkong (Vincent et al 2014), kapas (Dongre et al 2011), dan Eucalyptus (Subashini et al 2013). Konfirmasi pewarisan alel pada pisang dengan menggunakan marka SSR belum pernah dilakukan sebelumnya, akan tetapi keberhasilan yang ditunjukkan marka SSR pada tanaman lain dapat digunakan sebagai acuan untuk konfirmasi progeni hasil persilangan dalam studi genetik plasma nutfah pisang.…”