Confirmation of Alleles Inheritance in F1 Progenies Derived from a Cross of Calcutta-4 [Musa acuminata ssp. burmannicoides] and Musa acuminata ssp. microcarpa Based on SSR Markers

Abstract

<p>Banana breeding to produce improved varieties with disease resistance haracters and other desired traits could sustain its yield. Alleles harbored by parents could be passed on to the offsprings through hybridization, but need to be confirmed using molecular markers. This study aimed to confirm allele inheritance in F1 progenies derived from a cross of Calcutta-4 (Musa acuminata ssp. burmannicoides) and M. acuminata ssp. microcarpa based on SSR markers. Eleven pairs of SSR primers were used to amplify DNA of 44 progenies using the PCR technique. The results showed that six SSR markers (MaSSR 1.1, MaSSR<br />5.1, MaSSR 6.1, MaSSR 7.1, MaSSR 8.1, and MaSSR 11.1) were polymorphic in both parents. Four markers (MaSSR 1.1, MaSSR 5.1, MaSSR 6.1, and MaSSR 8.1) had PIC >0.7, indicating their informativeness to distinguish these progenies and other genetic studies of banana germplasms. A total of 44 F1 individuals were confirmed to harbor alleles inherited from their parents,<br />suggesting as true progenies from the cross of Calcutta-4 and M. acuminata ssp. microcarpa. This population demonstrated 100% success of hybridization performed. Chi-Square analysis revealed that segregation of all markers did not match to Mendelian ratio 1:2:1, except for MaSSR 1.1 (x2 = 5,62) and MaSSR 6.1 (x2 = 3,77) markers. The genetic traceability of banana F1 progenies demonstrating the usefulness and feasibility of SSR markers in this study provided information on selection of true progenies which may be valuable for breeders to assist selection process in future banana breeding program in Indonesia.</p>

Determination of The Parents Based on Molecular Analysis for Soybean Lines Development

“…Selain itu, penanda SSR dapat digunakan dalam analisis genetik karena menunjukkan tingkat variasi alelik dan dapat direproduksi. Penanda SSR juga dilaporkan merupakan penanda kodominan yang dapat diterapkan praktis untuk mendukung program pemuliaan tanaman (Reflinur & Lestari, 2015), antara lain keberhasilan persilangan pada generasi F1 tanaman pisang (Rosalia et al, 2020).…”

“…Sejak berkembangnya seleksi berbasiskan marka molekuler, seleksi molekuler memiliki keunggulan-keunggulan yaitu dapat mencapai sasaran target secara akurat, tidak dipengaruhi oleh lingkungan, tidak merusak karena hanya memerlukan bagian kecil dari tanaman dan dapat dilakukan pada fase bibit (Reflinur & Lestari, 2015;Santoso et al, 2006). Kesuksesan hibridisasi dapat diketahui oleh munculnya alelalel yang terbawa hasil introgresi dari kedua tetuanya sehingga generasi keturunannya tersebut terkonfirmasi sebagai tanaman hibrida (Rosalia et al, 2020).…”