Zero Waste is a goal that is ethical, economical, efficient and visionary, to guide people in changing their lifestyles and practices to emulate sustainable natural cycles, where all discarded materials are designed to become resources for others to use. In coastal area of Banggai, economic activities in fisheries with zero waste approach is implemented also to increase the added value of fish. Fish processing activities into food products cannot be separated from the waste generated. Waste generated from fish processing is in the form of bone, head, and entrails of fish. The amount and intensity of waste that appears can be reduced by applying the concept of zero waste through optimizing the use of waste generated during the fish processing process. The application of the zero waste concept provides benefits and reduces waste handling activities. From processing activities, there was an increase in the added value of Rp. 34,079,315.00. If calculated as an increase in income for members, this activity increases income by Rp. 236,661.91 per member per month or 10.10% of the UMR of Banggai Regency (Rp.2,343,970,-), and the ability to prevent waste generation by 75.61 kg per year.
<p>Swampland is a prospective marginal land to be developed for rice cultivation. Rice plants that are adaptive to swampland usually have good tolerance to Fe. Previously, we obtained several rice lines tolerant to Fe toxicity in the field. This study aimed to analyze the swampy rice genotypes using STS molecular markers linked to bacterial leaf blight (BLB)-resistant genes and evaluate the resistance level of the genotypes against three BLB races under the greenhouse condition. This research was conducted in the Molecular Biology Laboratory and Greenhouse of the Indonesian Center for Agricultural Biotechnology and<br />Genetic Resources Research and Development from January to May 2021. Eighteen swampy rice varieties/lines and two check varieties for Fe toxicity and BLB, respectively, were used in this research. The eight STS molecular markers linked to BLB resistance genes were used for genotyping analysis. The results showed that all rice plants tested contained xa5 allele gene, 19 genotypes had Xa4 allele gene, 17 genotypes had Xa7 allele gene, 9 genotypes had xa13 allele gene, 5 genotypes had xa13 allele gene, and 6 genotypes had Xa4 and Xa21 allele genes. Line B146E-4 was estimated to have 7 resistance alleles genes, meanwhile lines B14301E-KA-17-a, B14315E-KA-1, and B13-1-a have 6 resistance allele genes. The resistance evaluation of the genotypes against the three BLB races (races III, IV, and VIII) under the greenhouse condition showed that line B14315E-KA-1 was confirmed to be highly resistant to BLB disease. This line can be a promising candidate for swampy rice that is resistant to<br />BLB disease.</p>
This study investigates the intellectual endeavor to revive Islamic tradition and thoughts on nature conservation by using scientific narration and reasoning. The intellectual exercise, which is made by the Center for Islamic Studies Universitas Nasional (PPI-Unas), Jakarta that leads to various actions on nature conservation, from protecting the forest and endangered wildlife from poaching, preventing forest fire, and reducing CO2 emission to fighting climate change. The temporary result of these efforts to integrate Islamic teaching and sciences is then translated into 42 social and environmental projects and has been drawing support at home and overseas, academic circles, research institutions, donors, and NGOs. The obvious indication of international support is the accreditation of the PPI-Unas by the United Nations Environment Program (UNEP). Conclusion: At the practical level, modern sciences and Islamic teaching on nature conservation are complementing each other.
Soybean is the third most important food commodity in Indonesia, which is a cheap source of protein and rich in different nutritional contents for humans. This study aimed to analyze the four genotypes of the crossing parents using SSR primers and select one SSR polymorphic primer to confirm the F1 generation alleles compared to their parents. The research was conducted in the laboratory and greenhouse of the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD). The research activities included a polymorphic primers survey, population formation, and confirmation of crossing populations using one polymorphic primer. A total of 20 SSR primers were used to amplify the DNA of the four crossing parents (Biosoy 1, Biosoy 2, Demas, and Tanggamus). The results of the polymorphic SSR survey showed that 6 SSR primers could distinguish the combination of Biosoy 2 vs Demas parents, then 7 SSR primers could distinguish the combination of Biosoy 1 vs Tanggamus and Biosoy 2 vs Tanggamus parents. Satt 406 polymorphic primer was chosen to analyze F1 hybrid lines of three crossings. Based on phenotypic observation, two individuals were suspected to be hybrid lines. Molecular analysis using Satt 406 showed that alleles from male parents were not found in 16 F1 individuals from the three crossings. Selection using molecular markers such as Satt 406 polymorphic SSR can help breeders screen heterozygous populations in F1 generations to check successful crossings.Keywords: biosoy 1; biosoy 2; Demas; Tanggamus; Al tolerance; SSR markers ABSTRAKPenentuan Tetua Berbasis Analisis Molekuler Untuk Pembentukan Galur KedelaiKedelai merupakan komoditas pangan penting ketiga di Indonesia yang dimanfaatkan sebagai sumber protein yang murah dan kaya berbagai kandungan gizi bagi manusia. Tujuan penelitian ini adalah untuk untuk menganalisis 4 genotip tetua persilangan menggunakan primer SSR dan memilih satu primer polimorfik SSR untuk mengonfirmasi alel-alel generasi F1 dibandingkan dengan para tetuanya. Penelitian dilakukan di laboratorium dan rumah kaca Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian (BB Biogen). Kegiatan penelitian terdiri dari survei primer polimorfik, pembentukan populasi, dan konfirmasi populasi persilangan menggunakan satu primer polimorfik. Sebanyak 20 primer SSR digunakan untuk mengamplifikasi DNA dari empat tetua persilangan kedelai (Biosoy 1, Biosoy 2, Demas, dan Tanggamus). Hasil survei polimorfisme SSR menunjukkan bahwa 6 primer SSR dapat membedakan kombinasi tetua Biosoy 2 vs Demas, serta 7 primer SSR dapat membedakan Biosoy 1 vs Tanggamus dan Biosoy 2 vs Tanggamus. Primer polimorfik Satt 406 terpilih untuk menganalisis hibrida F1 dari tiga persilangan. Berdasarkan hasil pengamatan fenotipik, diperoleh 2 nomor individu F1 yang diduga sebagai generasi hibrida. Analisis molekuler menggunakan Satt 406 menunjukkan bahwa alel-alel dari tetua jantan tidak ditemukan pada 16 nomor tanaman dari 3 populasi persilangan. Seleksi menggunakan marka molekuler seperti SSR polimorfik Satt 406 membantu pemulia dalam menskrining populasi heterozigot pada generasi F1 untuk mengetahui keberhasilan persilangan.Kata kunci: biosoy 1, biosoy 2, Demas, Tanggamus, toleransi Al, marka SSR
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