Identification of expressed sequence tags in an alkali grass (Puccinellia tenuiflora) cDNA library

Physiological characterization, transcriptomic profiling, and microsatellite marker mining of Lycium ruthenicum

“…As a result, unigenes encoding certain transcription factors including dehydration responsive element binding protein (DREB), myeloblastosis (MYB), and WRKY were overrepresented in the 300 unigenes. This result was consistent with the findings that high salinity and alkalinity could trigger the expression of specific families of transcription factors, which consequently activates the downstream stress-responsive genes correspondingly (Zhu, 2001;Wang et al, 2007). Kinases such as serine/threonine-protein kinase and cysteine-rich receptor-like protein kinase were overrepresented in the top 300 lists.…”

Characterization of two plasma membrane protein 3 genes (PutPMP3) from the alkali grass, Puccinellia tenuiflora, and functional comparison of the rice homologues, OsLti6a/b from rice

“…Among these clones, we identified a full-length cDNA encoding a PMP3 homolog. Analyzing the data published by Wang et al (20), a 91-bp fragment from one of the EST clones (Accession number, CN487549) was found to have high sequence similarity with the PMP3 gene. The full-length cDNA was obtained with 3' and 5' RACE.…”

“…The PutPMP3-2 cDNA was identified directly from the EST clones sequenced. A part of PutPMP3-1 cDNA was amplified according to published data (GenBank accession number, CN487549) (20), and the full-length cDNA was obtained following 3' and 5' RACE methods. The sequences were aligned using the BioEdit software.…”

“…P. tenuiflora appears to have developed an efficient stress tolerance system and is a potential source of genetic determinants for saline tolerance (19)(20)(21). Under NaHCO3 stress, nearly 20 % of the expressed sequence tags from P. tenuiflora shared significant similarities with rice (20,21).…”

Molecular Breeding for Enhancing Abiotic Stress Tolerance Using Halophytes

“…Identification and isolation of novel genes by genomic approaches will advance the understanding of these mechanisms [24]. Hence, different efforts in large-scale ESTsequencing and analysis have been made in a number of dicotyledonous halophytes, such as Suaeda salsa [25], Thellungiella halophila [26], Mesembryanthemum crystallinum [27], Aegiceras corniculatum [28], Avicennia marina [29], Tamarix hispida [30], and Limonium sinense [31], and monocotyledonous halophytes such as Leymus chinensis [32], Puccinellia tenuiflora [33], Lolium temulentum [34], Aeluropus littoralis [35], and Spartina alterniflora [36].…”