Identification of exosome-like nanoparticle-derived microRNAs from 11 edible fruits and vegetables

Xiao, Juan; Feng, Siyuan; Wang, Xun; Long, Keren; Luo, Yi; Wang, Yuhao; Ma, Jideng; Tang, Qianzi; Jin, Long; Li, Xuewei; Li, Mingzhou

doi:10.7717/peerj.5186

Cited by 196 publications

(219 citation statements)

References 63 publications

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“…It is necessary to establish the possibilities for these miRNAs to enter the human and animal organisms. Several studies have shown that miRNAs in various parts of plants are present in exosomes 30-400 nm in size and are distributed in the body as part of these nanoparticles (Bang & Thum, 2012; Denzer et al, 2000; Xiao et al, 2018). Such compaction of miRNAs in exosomes contributes to their conservation and facilitates the entry of miRNAs into animals through the digestive tract (Redis et al, 2012; Théry, Zitvogel & Amigorena, 2002; Valadi et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Human Genes Are In Silico Potential Targets For Rice miRNA

Rakhmetullina

Pyrkova

Aisina

et al. 2020

Preprint

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16Exogenous miRNAs enter the human body through food, and their effects on metabolic 17 processes can be considerable. It is important to determine which miRNAs from plants 18 affect the expression of human genes and the extent of their influence. The binding sites of 19 738 osa-miRNAs that interact with 17508 mRNAs of human genes were determined using 20 the MirTarget program. The characteristics of the binding of 46 single osa-miRNAs to 86 21 mRNAs of human genes with a value of free energy (ΔG) interaction equal 94% to 100% 22 from maximum ΔG were established. The findings showed that osa-miR2102-5p, osa-23 miR5075-3p, osa-miR2097-5p, osa-miR2919 targeted the largest number of genes at 38, 36, 24 23, 19 sites, respectively. mRNAs of 86 human genes were identified as targets for 93 osa-25 miRNAs of all family osa-miRNAs with ΔG values equal 94% to 98% from maximum ΔG. 26Each miRNA of the osa-miR156-5p, osa-miR164-5p, osa-miR168-5p, osa-miR395-3p, osa-27 miR396-3p, osa-miR396-5p, osa-miR444-3p, osa-miR529-3p, osa-miR1846-3p, osa-28 miR2907-3p families had binding sites in mRNAs of several human target genes. The 29 binding sites of osa-miRNAs in mRNAs of the target genes for each family of osa-miRNAs 30 were conserved when compared to flanking nucleotide sequences. mRNA human genes of 31 osa-miRNAs are candidate genes of cancer, cardiovascular and neurodegenerative diseases.32 33 104genes. Each of the 35 miRNAs could bind to mRNAs of one target gene, six miRNAs had targets 105 with two genes, and four miRNAs had three target genes with a value ΔG/ΔGm equal to 94-106 98%. miR2102-5p had 11 target genes with a value ΔG/ΔGm of 94-100%, and the free energy of 107 the interaction of the miRNAs with the mRNAs of these genes varied from -115 kJ/mole to -121 108 4 kJ/mole. The miR2102-5p binding sites were located mainly in the 5'UTR, which suggests that 109 they have a role in the early inhibition of the translation process. However, this property of 110 miR2102-5p indicates the need to control its plant food-derived concentration in the human 111 body. 19 target genes were associated with miR2919. miR5075-3p could bind to three mRNAs at 112 binding sites located in the coding domain sequence and the 5'-untranslated region. The high-113 affinity binding sites were located in the 5'UTR and CDS of the mRNAs with the ΔG/ΔGm value 114 was 94-98%. 17 miRNA binding sites were located in 5'UTR, 39 in CDS and 33 in 3'-115 untranslated region. Therefore, monitoring the concentrations of miR2102-5p, miR2919 and 116 miR5075-3p in human biological fluids is also necessary. 117 118 122 development of cancer of various types: ENAH, MAPT, PRKCE, PRRT2, RBMS2, RHOBTB2, 123 RPS6KA5 and ZFHX3 -breast cancer (Aisina et al., 2019); ADAMTS5, PRAPRGG1A, PVR, 124 SPRY4 -colorectal cancer; CHSY1 -colorectal cancer and hepatocellular carcinoma; PPM1F, 125 FXYD6, DUT, FAM83H -hepatocellular carcinoma; HK2 -gallbladder cancer and leukaemia; 126 C19orf6 -ovarian carcinoma; AFAP1 -oesophageal adenocarcinoma; IOGAP1 -pancreatic 127 6 ductal adenoca...

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Section: Discussionmentioning

confidence: 99%

Human Genes Are In Silico Potential Targets For Rice miRNA

Rakhmetullina

Pyrkova

Aisina

et al. 2020

Preprint

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“…The same authors also speculate that exosomes liberated from digested edible plants may be a way of cross-species communication. Recently, plant derived exosome-like nanovescicles, having a structure similar to those of mammalian exosomes, have been characterized in different edible plant [90]. These nanovescicles can be absorbed in the mammalian gastrointestinal tract and have the potential to mediate plant-animal intercellular communication [91,92].…”

Section: Mirnas and Evolutionmentioning

confidence: 99%

Small RNAs in eucaryotes: new clues for amplifying microRNA benefits

et al. 2020

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miRNAs, the smallest nucleotide molecules able to regulate gene expression at post transcriptional level, are found in both animals and plants being involved in fundamental processes for growth and development of living organisms. The number of miRNAs has been hypothesized to increase when some organisms specialized the process of mastication and grinding of food. Further to the vertical transmission, miRNAs can undergo horizontal transmission among different species, in particular between plants and animals. In the last years, an increasing number of studies reported that miRNA passage occurs through feeding, and that in animals, plant miRNAs can survive the gastro intestinal digestion and transferred by blood into host cells, where they can exert their functions modulating gene expression. The present review reports studies on miRNAs during evolution, with particular focus on biogenesis and mechanisms regulating their stability in plants and animals. The different biogenesis and post biogenesis modifications allow to discriminate miRNAs of plant origin from those of animal origin, and make it possible to better clarify the controversial question on whether a possible cross-kingdom miRNA transfer through food does exist. The majority of human medicines and supplements derive from plants and a regular consumption of plant food is suggested for their beneficial effects in the prevention of metabolic diseases, cancers, and dietary related disorders. So far, these beneficial effects have been generally attributed to the content of secondary metabolites, whereas mechanisms regarding other components remain unclear. Therefore, in light of the above reported studies miRNAs could result another component for the medical properties of plants. miRNAs have been mainly studied in mammals characterizing their sequences and molecular targets as available in public databases. The herein presented studies provide evidences that miRNA situation is much more complex than the static situation reported in databases. Indeed, miRNAs may have redundant activities, variable sequences, different methods of biogenesis, and may be differently influenced by external and environmental factors. In-depth knowledge of mechanisms of synthesis, regulation and transfer of plant miRNAs to other species can open new frontiers in the therapy of many human diseases, including cancer.

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“…In reference to the current efforts to use EXs as drug delivery systems, it should be highlighted that despite the identified very high organotropism of EXs derived from cancer cells, a shift to safer alternatives such as EXs produced from foods, has been recently made in this research field. From all the relevant sources of this category, bovine milk-and fruit-derived EXs are probably the most investigated for the purpose [72]. Indeed, bovine milk has been proposed as a scalable source of exosomes that can act as a carrier for chemotherapeutic/chemopreventive agents [73].…”

Section: Sources Of Exosomesmentioning

confidence: 99%

Exosomes and Exosome-Inspired Vesicles for Targeted Drug Delivery

Antimisiaris

Mourtas

Marazioti

2018

Preprint

125

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The similarities between exosomes and liposomes, together with the high organotropism of several types of exosomes, have recently prompted the development of engineered-exosomes or exosome-mimetics, which may be artificial (liposomal) or cell-derived vesicles, as advanced platforms for targeted drug delivery. Here we provide the current state-of-the-art of using exosome or exosome-inspired systems for drug delivery. We review the various approaches investigated and the shortcomings of each approach. Finally the challenges identified up-to-date in this field are summarized.

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Identification of exosome-like nanoparticle-derived microRNAs from 11 edible fruits and vegetables

Cited by 196 publications

References 63 publications

Human Genes Are In Silico Potential Targets For Rice miRNA

Human Genes Are In Silico Potential Targets For Rice miRNA

Small RNAs in eucaryotes: new clues for amplifying microRNA benefits

Exosomes and Exosome-Inspired Vesicles for Targeted Drug Delivery

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