Human Genes Are In Silico Potential Targets For Rice miRNA

Abstract

16Exogenous miRNAs enter the human body through food, and their effects on metabolic 17 processes can be considerable. It is important to determine which miRNAs from plants 18 affect the expression of human genes and the extent of their influence. The binding sites of 19 738 osa-miRNAs that interact with 17508 mRNAs of human genes were determined using 20 the MirTarget program. The characteristics of the binding of 46 single osa-miRNAs to 86 21 mRNAs of human genes with a value of free energy (ΔG) interaction equal 94% to 100% 22 from maximum ΔG were established. The findings showed that osa-miR2102-5p, osa-23 miR5075-3p, osa-miR2097-5p, osa-miR2919 targeted the largest number of genes at 38, 36, 24 23, 19 sites, respectively. mRNAs of 86 human genes were identified as targets for 93 osa-25 miRNAs of all family osa-miRNAs with ΔG values equal 94% to 98% from maximum ΔG. 26Each miRNA of the osa-miR156-5p, osa-miR164-5p, osa-miR168-5p, osa-miR395-3p, osa-27 miR396-3p, osa-miR396-5p, osa-miR444-3p, osa-miR529-3p, osa-miR1846-3p, osa-28 miR2907-3p families had binding sites in mRNAs of several human target genes. The 29 binding sites of osa-miRNAs in mRNAs of the target genes for each family of osa-miRNAs 30 were conserved when compared to flanking nucleotide sequences. mRNA human genes of 31 osa-miRNAs are candidate genes of cancer, cardiovascular and neurodegenerative diseases.32 33 104genes. Each of the 35 miRNAs could bind to mRNAs of one target gene, six miRNAs had targets 105 with two genes, and four miRNAs had three target genes with a value ΔG/ΔGm equal to 94-106 98%. miR2102-5p had 11 target genes with a value ΔG/ΔGm of 94-100%, and the free energy of 107 the interaction of the miRNAs with the mRNAs of these genes varied from -115 kJ/mole to -121 108 4 kJ/mole. The miR2102-5p binding sites were located mainly in the 5'UTR, which suggests that 109 they have a role in the early inhibition of the translation process. However, this property of 110 miR2102-5p indicates the need to control its plant food-derived concentration in the human 111 body. 19 target genes were associated with miR2919. miR5075-3p could bind to three mRNAs at 112 binding sites located in the coding domain sequence and the 5'-untranslated region. The high-113 affinity binding sites were located in the 5'UTR and CDS of the mRNAs with the ΔG/ΔGm value 114 was 94-98%. 17 miRNA binding sites were located in 5'UTR, 39 in CDS and 33 in 3'-115 untranslated region. Therefore, monitoring the concentrations of miR2102-5p, miR2919 and 116 miR5075-3p in human biological fluids is also necessary. 117 118 122 development of cancer of various types: ENAH, MAPT, PRKCE, PRRT2, RBMS2, RHOBTB2, 123 RPS6KA5 and ZFHX3 -breast cancer (Aisina et al., 2019); ADAMTS5, PRAPRGG1A, PVR, 124 SPRY4 -colorectal cancer; CHSY1 -colorectal cancer and hepatocellular carcinoma; PPM1F, 125 FXYD6, DUT, FAM83H -hepatocellular carcinoma; HK2 -gallbladder cancer and leukaemia; 126 C19orf6 -ovarian carcinoma; AFAP1 -oesophageal adenocarcinoma; IOGAP1 -pancreatic 127 6 ductal adenoca...

The Impact of Dietary Compounds in Functional Foods on MicroRNAs Expression

“…Cancer, cardiovascular and neurodegenerative diseases [119] Table 2. Dietary miRNAs and human gene regulation.…”

“…The JAM-A was up-regulated in atherosclerotic lesions from cardiovascular disease patients and miR156a could suppressed inflammatory cytokine-induced monocytes adhesion by targeting JAM-A [118]. The very recently report using a computational approach to predict the potential target of rice miRNAs including miR156-5p, miR164-5p, miR168-5p, miR395-3p, miR396-3p, miR396-5p, miR444-3p, miR529-3p, miR1846-3p, miR2907-3p, which can bind to the human mRNA [119]. Most of these target genes were associated with cancer, cardiovascular and neurodegenerative diseases [119].…”

“…The very recently report using a computational approach to predict the potential target of rice miRNAs including miR156-5p, miR164-5p, miR168-5p, miR395-3p, miR396-3p, miR396-5p, miR444-3p, miR529-3p, miR1846-3p, miR2907-3p, which can bind to the human mRNA [119]. Most of these target genes were associated with cancer, cardiovascular and neurodegenerative diseases [119]. miR14 derived from Curcuma longa was detected and remarkably stable in human serum for 48 h. The potential targets of miR14 were associated with inflammation in rheumatoid arthritis such as Phosphotidylinositol-specific-phospholipase C (PLCXD3), Adenylate cyclase 9 (ADCY9), and 3′ (2′), 5′-bisphosphate nucleotidase (BPNT1) [120].…”