Identification of Epstein-Barr virus terminal protein 1 (TP1) in extracts of four lymphoid cell lines, expression in insect cells, and detection of antibodies in human sera

Frech, Barbara; Zimber-Strobl, Ursula; Suentzenich, K O; Pavlish, O; Lenoir, Gilbert; Bornkamm, G. W.; Mueller‐Lantzsch, Nikolaus

doi:10.1128/jvi.64.6.2759-2767.1990

Cited by 41 publications

(18 citation statements)

References 35 publications

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“…This suggests that, when suitable serological reagents are available, the LMP2A protein will be regularly detectable in NPC. In this context, we note with interest that a recent serological survey has reported a high prevalence of LMP2-specific antibodies in the sera of NPC patients (10).…”

Section: Discussionmentioning

confidence: 94%

Epstein-Barr virus latent gene transcription in nasopharyngeal carcinoma cells: coexpression of EBNA1, LMP1, and LMP2 transcripts

Brooks

Yao

Rickinson

et al. 1992

J Virol

429

163

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Epstein-Barr virus (EBV) genome-positive nasopharyngeal carcinomas (NPCs) regularly express the virus-coded nuclear antigen EBNAI, but not other EBNAs, and a subset of tumors also appear to be latent membrane protein LMP1 positive; the status of NPCs with respect to a second virus-coded latent membrane protein LMP2 is unknown. In the present work the EBV-NPC cell interaction has been analyzed at the RNA level with reverse transcription and polymerase chain reaction-based amplification to detect specific latent viral mRNAs. All four transplantable NPC cell lines studied and 17 of 18 fresh snap-frozen NPC biopsy specimens expressed an EBNAl mRNA with a BamHI Q/U/K splice structure exactly like that recently identified in group I Burkitt's lymphoma (BL) cell lines and shown to be driven from a novel viral promoter, Fp. The BamHI Y3/U/K-spliced EBNA1 mRNA characteristic of virus-transformed B-lymphoblastoid cell lines (LCLs) was never found in NPCs. These same NPC biopsy specimens were then analyzed for evidence of the various LMP transcripts which are constitutively expressed in LCLs but down-regulated in BL cells. While only 3 of 18 tumors gave a clear LMP1 mRNA-specific signal after first-round amplification with either of two sets of polymerase chain reaction primers, the majority proved to be LMP1 mRNA positive after second-round amplification with nested primers. A rather similar pattern of results was obtained with respect to LMP2B mRNA expression, such transcripts being detectable only in a subset of tumors, and then at apparently low levels. In contrast, clear evidence of LMP2A mRNA expression was obtained in 17 of 18 fresh biopsies. The predominant form of EBV infection in NPCs, with coexpression of EBNA1 and LMP mRNAs, is therefore quite distinct from that seen in BL cells (in which EBNA1 is the only expressed mRNA) and in LCL cells (in which all six EBNA and three LMP transcripts are present). This third form of EBV latency may not be restricted to NPC but may have more general relevance in the context of EBV infection in vivo.

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Section: Discussionmentioning

confidence: 94%

Epstein-Barr virus latent gene transcription in nasopharyngeal carcinoma cells: coexpression of EBNA1, LMP1, and LMP2 transcripts

Brooks

Yao

Rickinson

et al. 1992

J Virol

429

163

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“…(There is no evidence that the latter exist.) The structure of two TP transcripts has been elucidated (12,15) and recently, two differently-sized TP proteins have been demonstrated to exist in B-cells (16,17). We have found the region encoding the TP gene to be transcriptionally active in the NPC tumours examined, as well as in a B-cell line established with rescued virus from one of the tumours.…”

Section: Introductionmentioning

confidence: 83%

“…The terminal protein (TP) genes-TP1 and TP2-encoded by the fused terminal repeats of the viral genome prove to be an instructive model for studying control of viral gene expression in EBV-carrying cells. Although biological functions for these two related genes have not yet been identified, they use different promoters and specify differently-sized (40 and 53kD) proteins (16,17). Presumably these proteins have different activities.…”

Section: Discussionmentioning

confidence: 99%

Differential expression of Epstein Barr viral transcripts for two proteins (TP1 and LMP) in lymphocyte and epithelial cells

Smith

Griffin

1991

Nucl Acids Res

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Studies presented here show that some functions of the human herpesvirus, EBV, may be transcriptionally differentially expressed in two cell types which carry the same (C15) isolate of this virus. Of the 'latent' viral functions investigated, only one (TP2) of the episomally-specific genes that encode terminal proteins (TP1 and TP2) is found to be expressed in the C15 epithelial cell tumour environment, whereas both are transcribed--as different, but related, messengers--in a B-cell line generated with virus from the C15 tumour. The other gene investigated is that for latent membrane protein (LMP), which is found in the same region of the EBV genome but on the opposite strand. This gene, apparently transcriptionally silent in B-cell (Burkitt's) lymphomas, is expressed in the C15 epithelial tumour, as well as in other nasopharyngeal carcinomas investigated. Promoter usage in the carcinomas and B-cells appears, in some cases at least, to be cell-type specific. Expression may also be governed by methylation since a chromosomally silent region in the carcinoma (that encompassing TP1) is highly methylated on CpG residues, whereas the active region (encoding TP2 and LMP) is virtually free of such methylation. Our data suggest that there may be selective transcriptional regulation of EBV genes in the two types of cells investigated. Thus, it may be unnecessary to invoke different virus genotypes to account for the two distinct malignancies--Burkitt's lymphoma and nasopharyngeal carcinoma--associated with EBV.

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“…Epstein-Barr virus (EBV) is a human herpesvirus which infects B lymphocytes in vivo or in vitro (34,46). EBV establishes a latent infection in B lymphocytes in which EBV expresses a set of six nuclear proteins (34, 46) and two integral plasma membrane proteins, LMP1 (21,26) and LMP2 (22,40,44,54,55). LMP1 and LMP2 have 6 and 12 hydrophobic transmembrane domains, respectively.…”

mentioning

confidence: 99%

An Epstein-Barr virus transformation-associated membrane protein interacts with src family tyrosine kinases

Burkhardt

Bolen

Kieff

et al. 1992

J Virol

124

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In latently infected growth-transformed human lymphocytes, Epstein-Barr virus (EBV) encodes two integral plasma membrane proteins: LMP1, which constitutively induces B-lymphocyte activation and intercellular adhesion, and LMP2A, which associates with LMP1 and is a tyrosine kinase substrate. We now demonstrate that LMP2A associates with src family protein tyrosine kinases, particularly lyn kinase, in nonionic detergent extracts of transfected B lymphoma cells or in extracts of EBV-transformed B lymphocytes. The LMP2A and tyrosine kinase association is stable in nonionic detergents and includes a 70-kDa cell protein which is also an in vitro or in vivo kinase substrate. This LMP2A association with B-lymphocyte src family tyrosine kinases is likely to be an important pathway in EBV's effects on cell growth.

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Identification of Epstein-Barr virus terminal protein 1 (TP1) in extracts of four lymphoid cell lines, expression in insect cells, and detection of antibodies in human sera

Cited by 41 publications

References 35 publications

Epstein-Barr virus latent gene transcription in nasopharyngeal carcinoma cells: coexpression of EBNA1, LMP1, and LMP2 transcripts

Epstein-Barr virus latent gene transcription in nasopharyngeal carcinoma cells: coexpression of EBNA1, LMP1, and LMP2 transcripts

Differential expression of Epstein Barr viral transcripts for two proteins (TP1 and LMP) in lymphocyte and epithelial cells

An Epstein-Barr virus transformation-associated membrane protein interacts with src family tyrosine kinases

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