Identification of epitopes on nonstructural protein 7 of porcine reproductive and respiratory syndrome virus recognized by monoclonal antibodies using phage-display technology

Wang, Heng; Liu, Rongchang; Zhang, Weidong; Sun, Li; Ning, Zhangyong; Ji, Fangxiao; Cui, Jin; Zhang, Guihong

doi:10.1007/s11262-017-1472-9

Cited by 4 publications

(5 citation statements)

References 46 publications

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“…In recent years, a PRRSV mAb has been successfully produced, and many researchers have prepared PRRSV mAbs targeting a certain structural or non-structural protein found in different types of PRRSV. The nonstructural proteins include NSP2 ( Yan et al, 2007 ; Wang et al, 2008 ; Hu et al, 2009 ; Li et al, 2013 ; Wang H. et al, 2017 ), NSP7 ( Wang F.-X. et al, 2017 ), NSP9 ( Wu et al, 2012 ; Zhao et al, 2016 ), NSP10 ( Zhang Z. et al, 2017 ), and NSP12 ( Bi et al, 2017 ); the structural proteins include GP2 ( Liu et al, 2006 ), GP3 ( Sun et al, 2013 ; Liang et al, 2021 ), GP4 ( Guo et al, n.d. ; Liu et al, 2019 ), GP5 ( Zhou et al, 2005a , b ; Ma et al, 2006 , 2007 , 2008 ; Cai et al, 2009 ; Wang et al, 2009 , 2012 ; Zhong et al, 2011 ; Tang et al, 2013 ; Li et al, 2016 , 2019 ; Huang et al, 2019 ; Yang et al, 2020 ; Qin et al, 2021 ; Young et al, 2021 ; Wang F. et al, 2022 ; Supplementary Table S1 ).…”

Section: Serological Detection Methodsmentioning

confidence: 99%

“…The commonly used HP-PRRSV vaccines have poor cross-protection against NADC30-like PRRSV ( Wu et al, 2021 ; Zhao et al, 2022 ). The RT-PCR assays developed by Xu and Zhu (2019) , Rao (2020) , and Wang M. et al (2022) are able to detect NADC30-like PRRSV to essentially solve the above situation. The detection rate of NADC34-like PRRSV as a potentially prevalent strain is increasing.…”

Section: Molecular Biology Detection Methodsmentioning

confidence: 99%

“…The RT-PCR assays developed by Xu and Zhu (2019), Rao (2020), and Wang M. et al (2022) are able to detect NADC30-like PRRSV to essentially solve the above situation. The detection rate of NADC34-like PRRSV as a potentially prevalent strain is increasing.…”

Section: Protein Of Target Application Referencesmentioning

confidence: 99%

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Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus

Pan

Zeng²,

Zhao³

et al. 2023

Front. Microbiol.

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Porcine reproductive and respiratory syndrome virus (PRRSV) causes clinical syndromes typified as reproductive disorders in sows and respiratory diseases in piglets. PRRSV remains one of the most prevalent pathogens affecting the pig industry, because of its complex infection profile and highly heterogeneous genetic and recombination characteristics. Therefore, a rapid and effective PRRSV detection method is important for the prevention and control of PRRS. With extensive in-depth research on PRRSV detection methods, many detection methods have been improved and promoted. Laboratory methods include techniques based on virus isolation (VI), enzyme-linked immunosorbent assays (ELISA), indirect immunofluorescence assays (IFA), immunoperoxidase monolayer assays (IPMA), polymerase chain reaction (PCR), quantitative real-time PCR (qPCR), digital PCR (dPCR), loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), clustered regularly interspaced short palindromic repeats (CRISPR), metagenomic next-generation sequencing (mNGS), and other methods. This study reviews the latest research on improving the main PRRSV detection methods and discusses their advantages and disadvantages.

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Section: Serological Detection Methodsmentioning

confidence: 99%

Section: Molecular Biology Detection Methodsmentioning

confidence: 99%

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Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus

Pan

Zeng²,

Zhao³

et al. 2023

Front. Microbiol.

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“…Phages carrying NSP7 peptides have the potential to be used as PRRSV diagnostic reagents and antiviral drugs. The epitope distribution of the PRRSV NSP7 protein was also screened using phage display technology [56]. A new linear epitope, NAWGDEDRLN, was found on the PRRSV-2 NSP7β subunit at amino acids 153-162, which showed good reactivity with PRRSV-positive serum.…”

Section: Application Of Nsp7 In Prrsv Diagnosismentioning

confidence: 99%

Research Progress on Porcine Reproductive and Respiratory Syndrome Virus NSP7 Protein

Luo

Jing

et al. 2023

Animals

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Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious and severe infectious disease caused by the PRRS virus (PRRSV). PRRS is characterized by reproductive disorders in sows and respiratory dysfunction in pigs. Non-structural protein 7 (NSP7) is one of the most conserved functional proteins in PRRSV, and it plays an important role in viral replication and humoral immune responses in infected hosts. This review discusses the biological characteristics of NSP7 to provide theoretical support for its application in PRRS diagnosis, novel vaccine design, and therapeutic drug development.

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“…Recently, several methods of epitope prediction have been in use, the majority of which are limited to one antigen, although they still provide a satisfactory predictive capacity (7–9). X-ray diffraction requires more time and energy to identify epitope structures.…”

Section: Introductionmentioning

confidence: 99%

H1N1 influenza virus epitopes classified by monoclonal antibodies

et al. 2018

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Epitopes serve an important role in influenza infection. It may be useful to screen universal influenza virus vaccines, analyzing the epitopes of multiple subtypes of the hemagglutinin (HA) protein. A total of 40 monoclonal antibodies (mAbs) previously obtained from flu virus HA antigens (development and characterization of 40 mAbs generated using H1N1 influenza virus split vaccines were previously published) were used to detect and classify mAbs into distinct flu virus sub-categories using the ELISA method. Following this, the common continuous amino acid sequences were identified by multiple sequence alignment analysis with the GenBank database and DNAMAN software, for use in predicting the epitopes of the HA protein. Synthesized peptides of these common sequences were prepared, and used to verify and determine the predicted linear epitopes through localization and distribution analyses. With these methods, nine HA linear epitopes distributed among different strains of influenza virus were identified, which included three from influenza A, four from 2009 H1N1 and seasonal influenza, and two from H1. The present study showed that considering a combination of the antigen-antibody reaction specificity, variation in the influenza virus HA protein and linear epitopes may present a useful approach for designing effective multi-epitope vaccines. Furthermore, the study aimed to clarify the cause and pathogenic mechanism of influenza virus HA-induced flu, and presents a novel idea for identifying the epitopes of other pathogenic microorganisms.

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Identification of epitopes on nonstructural protein 7 of porcine reproductive and respiratory syndrome virus recognized by monoclonal antibodies using phage-display technology

Cited by 4 publications

References 46 publications

Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus

Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus

Research Progress on Porcine Reproductive and Respiratory Syndrome Virus NSP7 Protein

H1N1 influenza virus epitopes classified by monoclonal antibodies

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