Identification of Enterococcus mundtii as a pathogenic agent involved in the “flacherie” disease in Bombyx mori L. larvae reared on artificial diet

Cappellozza, Silvia; Saviane, Alessio; Tettamanti, Gianluca; Squadrin, Marta; Vendramin, Elena; Paolucci, Paolo; Franzetti, Eleonora; Squartini, Andrea

doi:10.1016/j.jip.2010.12.007

Cited by 41 publications

(19 citation statements)

References 17 publications

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“…Cappellozza et al reported previously the infection of silkworm larvae reared on artificial diet by E. mundtii (10). Coupled with our observation, therefore, we suspected that the origin of E. mundtii was artificial diet.…”

Section: Isolation Of Bacteria From Artificial Diet Of Silkwormsupporting

confidence: 86%

“…There have been previous reports of infection of silkworm larvae by various pathogens vis-à-vis: Metarhizium anisopliae (6), Alphabaculovirus (7), parasitic Nosema species (8), and Bombyx densovirus type 1 (9). Similarly, Enterococcus mundtii (E. mundtii) has been shown to be directly associated with flacherie disease of the silkworm larvae reared on artificial diet (10). In this present study, we explored the origin of observed pathogenic infection in industrial and transgenic strains of silkworm larvae, used as a non-mammalian animal model in drug discovery, and possibility of preventing such infections.…”

Section: Introductionmentioning

confidence: 99%

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Identification and methods for prevention of Enterococcus mundtii infection in silkworm larvae, Bombyx mori, reared on artificial diet

Nwibo

Matsumoto

Sekimizu

2015

DD&T

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Section: Isolation Of Bacteria From Artificial Diet Of Silkwormsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Identification and methods for prevention of Enterococcus mundtii infection in silkworm larvae, Bombyx mori, reared on artificial diet

Nwibo

Matsumoto

Sekimizu

2015

DD&T

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“…Hence, the occurrence of AR genes in edible insects could be a direct consequence of these practices. As early as 1992, Peng and others () studied the use of chlortetracycline in laboratory‐reared honey bees; more recently, Hirose and others () described the use of streptomycin to prolong the life of the insect Nezara viridula , whereas Cappellozza and others () reported the use of chloramphenicol in mass‐rearing of silkworms to avoid loss of cocoons.…”

Section: Resultsmentioning

confidence: 99%

Transferable Antibiotic Resistances in Marketed Edible Grasshoppers (Locusta migratoria migratorioides)

Osimani

Garofalo

Aquilanti

et al. 2017

Journal of Food Science

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Grasshoppers are the most commonly eaten insects by humans worldwide, as they are rich in proteins and micronutrients. This study aimed to assess the occurrence of transferable antibiotic resistance genes in commercialized edible grasshoppers. To this end, the prevalence of 12 selected genes [aac(6')-Ie aph(2″)-Ia, blaZ, erm(A), erm(B), erm(C), mecA, tet(M), tet(O), tet(S), tet(K), vanA, vanB] coding for resistance to antibiotics conventionally used in clinical practice was determined. The majority of samples were positive for tet(M) (70.0%), tet(K) (83.3%) and blaZ (83.3%). A low percentage of samples were positive for erm(B) (16.7%), erm(C) (26.7%), and aac(6')-Ie aph(2″)-Ia (13.3%), whereas no samples were positive for erm(A), vanA, vanB, tet(O), and mecA. Cluster analysis identified 4 main clusters, allowing a separation of samples on the basis of their country of origin.

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“…The infection could be achieved at low E. mundtii concentrations added to the diet. The infection could also enter the body cavity after multiplying in the larval gut [34]. Lysenko used two kinds of methods to prove the pathogenicity of the Streptocossus strains on healthy silkworm larvae through feeding and injection [24].…”

Section: Discussionmentioning

confidence: 99%

Impact on Bacterial Community in Midguts of the Asian Corn Borer Larvae by Transgenic Trichoderma Strain Overexpressing a Heterologous chit42 Gene with Chitin-Binding Domain

Gao

et al. 2013

PLoS ONE

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This paper is the first report of the impact on the bacterial community in the midgut of the Asian corn borer (Ostrinia furnacalis) by the chitinase from the transgenic Trichoderma strain. In this study, we detected a change of the bacterial community in the midgut of the fourth instar larvae by using a culture-independent method. Results suggested that Proteobacteria and Firmicutes were the most highly represented phyla, being present in all the midgut bacterial communities. The observed species richness was simple, ranging from four to five of all the 16S rRNA clone libraries. When using Trichoderma fermentation liquids as additives, the percentages of the dominant flora in the total bacterial community in larval midgut changed significantly. The community of the genus Ochrobactrum in the midgut decreased significantly when the larvae were fed with the fermentation liquids of the transgenic Trichoderma strain Mc4. However, the Enterococcus community increased and then occupied the vacated niche of the Ochrobactrum members. Furthermore, the Shannon–Wiener (H) and the Simpson (1-D) indexes of the larval midgut bacterial library treated by feeding fermentation liquids of the transgenic Trichoderma strain Mc4 was the lowest compared with the culture medium, fermentation liquids of the wild type strain T30, and the sterile artificial diet. The Enterococcus sp. strain was isolated and characterized from the healthy larvae midgut of the Asian corn borer. An infection study of the Asian corn borer larvae using Enterococcus sp. ACB-1 revealed that a correlation existed between the increased Enterococcus community in the larval midgut and larval mortality. These results demonstrated that the transgenic Trichoderma strain could affect the composition of the midgut bacterial community. The change of the midgut bacterial community might be viewed as one of the factors resulting in the increased mortality of the Asian corn borer larvae.

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Identification of Enterococcus mundtii as a pathogenic agent involved in the “flacherie” disease in Bombyx mori L. larvae reared on artificial diet

Cited by 41 publications

References 17 publications

Identification and methods for prevention of <i>Enterococcus mundtii</i> infection in silkworm larvae, <i>Bombyx mori</i>, reared on artificial diet

Identification and methods for prevention of <i>Enterococcus mundtii</i> infection in silkworm larvae, <i>Bombyx mori</i>, reared on artificial diet

Transferable Antibiotic Resistances in Marketed Edible Grasshoppers (Locusta migratoria migratorioides)

Impact on Bacterial Community in Midguts of the Asian Corn Borer Larvae by Transgenic Trichoderma Strain Overexpressing a Heterologous chit42 Gene with Chitin-Binding Domain

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