Identification and methods for prevention of &lt;i&gt;Enterococcus mundtii&lt;/i&gt; infection in silkworm larvae, &lt;i&gt;Bombyx mori&lt;/i&gt;, reared on artificial diet

Nwibo, Don Daniel; Matsumoto, Yasuhiko; Sekimizu, Kazuhisa

doi:10.5582/ddt.2015.01036

Cited by 7 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This pathogen has been isolated from fresh ML or AD made from ML. When silkworms are infected with this pathogen, the larvae can become anorexic, thus affecting their development [ 29 , 30 ]. Among DEGs, the expression level of PGRP in the AD group was hundreds of times (KWMTBOMO09651: 295.69-fold down-regulation in the FG group and 291.39-fold down-regulation in the MG group) lower than that in the ML group, thus, indicating that, compared with that in the control group, the expression of the PGRP gene in the midgut in silkworms fed AD was significantly diminished; therefore, the antimicrobial ability of silkworms in the experimental group was significantly weakened.…”

Section: Discussionmentioning

confidence: 99%

Midgut and Head Transcriptomic Analysis of Silkworms Reveals the Physiological Effects of Artificial Diets

Chen

Zha

2022

Insects

View full text Add to dashboard Cite

Silkworms, a model lepidopteran insect, have a very simple diet. Artificial diets as an alternative nutrient source for silkworms are gradually being developed. To understand the effects of various nutrients on the growth and development of silkworms, we studied the transcriptomic differences in the midgut and head tissues of male and female silkworms fed either fresh mulberry leaves or artificial diets. In the artificial diet group, compared with the control group (fed mulberry leaves), 923 and 619 differentially expressed genes (DEGs) were identified from the midgut, and 2969 and 3427 DEGs were identified from the head, in female and male silkworms. According to our analysis, the DEGs were mainly involved in the digestion and absorption of nutrients and silkworm innate immunity. These experimental results provide insights into the effects of different foods, such as artificial diets or fresh mulberry leaves, on silkworms.

show abstract

Section: Discussionmentioning

confidence: 99%

Midgut and Head Transcriptomic Analysis of Silkworms Reveals the Physiological Effects of Artificial Diets

Chen

Zha

2022

Insects

View full text Add to dashboard Cite

show abstract

“…In our laboratory, silkworm eggs are treated with formalin and the larvae are fed an artificial diet containing antibiotics. Under such rearing conditions, viable bacteria and fungi are not detected on agar plates when the materials in the silkworm intestinal tract are spread and incubated (27). In the present study, we examined whether lactic acid bacteria can be recovered from the intestinal tract when silkworms are fed a diet supplemented with lactic acid bacteria (Table 1).…”

Section: Viability Of Orally Administered Lactic Acid Bacteria In Thementioning

confidence: 99%

Establishment of a gnotobiotic silkworm model

Nakajima¹,

Matsumoto

Sekimizu

2018

DD&T

Self Cite

View full text Add to dashboard Cite

Gnotobiotic animals are useful for investigation of the effects of specific lactic acid bacteria on individual animals. Here we report that lactic acid bacteria colonize and proliferate in the intestinal tract of germ-free silkworms. When silkworms hatching from formalin-treated eggs were reared to fifth-instar larvae with an artificial diet containing antibiotics, bacteria and fungi were not observed in their intestines. An antibiotic-free diet supplemented with viable lactic acid bacteria, such as Enterococcus faecalis 0831-07, Lactococcus lactis 11/19-B1, or Leuconostoc carnosum #7-2, was fed to the germ-free silkworms for 1 day. After feeding the larvae on a diet without lactic acid bacteria for 5 days, each type of lactic acid bacterium was found in the intestine. Moreover, an increase in the number of Enterococcus faecalis 0831-07 was observed in the intestine 2-5 days after ingestion. These findings suggest that we successfully established a method to construct a gnotobiotic silkworm model.

show abstract

“…This problem can be resolved by using silkworm as a model. Silkworms possess similar pharmacokinetics (Absorption, Distribution, Metabolism and Excretion) characteristics as mammals (Hamamoto, et al, 2009; Nwibo et al, 2015). When hepato‐toxic chemicals are injected into silkworm, it causes elevation of alanine aminotransferase (ALT), an essential biomarker for tissue injury, in the hemolymph of the silkworm (Inagaki et al, 2012; Inagaki et al, 2016).…”

Section: Silkworm B Mori As Antimicrobial Drug Screening and Pathogen...mentioning

confidence: 99%

“…These intriguing characteristics led researchers to study this insect not just as a silk‐producing organism, but also as an organism with the potential to serve as a model organism in life science research. The application of silkworm has emerged greatly in its use in drug screening and discovery (Hamamoto, et al, 2009; Matsumoto & Sekimizu, 2016; Nwibo et al, 2015), environmental safety, anti‐pathogenic drug screening, study of antiviral agents (Ishii et al, 2008) and toxicological research (Abdelli et al, 2018). This review covers areas related to human health and diseases where silkworm B. mori has been used as a model organism or has the potential of being so, and also bolsters the other review papers already published in this area by supplementing it with new knowledge.…”

Section: Introductionmentioning

confidence: 99%

Silkworm Bombyx mori as a model organism: A review

Ashraf,

Qamar

2023

Physiological Entomology

View full text Add to dashboard Cite

Bombyx mori Linnaeus, 1758 (Lepidoptera: Bombycidae), a very important economical insect and backbone of the silk industry, is fully reliant on humans for its life cycle. It has short life span, possessing many genes having high degree of homology with human disease‐causing genes, low breeding and maintenance cost, has less ethical issues associated with it and has also got its genome fully sequenced. Because of these characteristics, it has been recognized as an alternate invertebrate model organism candidate for use in life science research. It has been successfully used as an alternative invertebrate model organism in a variety of scientific domains, including human disease models, environmental monitoring models, epigenetic models and microbial drug screening and discovery models, since the last decade. This newly emerged model has given promising results so far in its short journey and has a tremendous future prospect of establishing itself as a successful model just like the classical invertebrate models, Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae) and Caenorhabditis elegans Maupas, 1900 (Rhabditida: Rhabditidae). The use of the silkworm B. mori as a model organism in areas linked to human health and disease is reviewed here.

show abstract

Identification and methods for prevention of <i>Enterococcus mundtii</i> infection in silkworm larvae, <i>Bombyx mori</i>, reared on artificial diet

Cited by 7 publications

References 18 publications

Midgut and Head Transcriptomic Analysis of Silkworms Reveals the Physiological Effects of Artificial Diets

Midgut and Head Transcriptomic Analysis of Silkworms Reveals the Physiological Effects of Artificial Diets

Establishment of a gnotobiotic silkworm model

Silkworm Bombyx mori as a model organism: A review

Contact Info

Product

Resources

About