Identification of dysregulated long non-coding RNAs/microRNAs/mRNAs in TNM I stage lung adenocarcinoma

Abstract

Lung adenocarcinoma (LUAD) is the primary subtype in lung cancer, which is the leading cause of cancer-related death worldwide. This study aimed to investigate the aberrant expression profiling of long non-coding RNA (lncRNA) in TNM I stage (stage I) LUAD. The lncRNA/mRNA/miRNA expression profiling of stage I LUAD and adjacent non-tumor tissues from 4 patients were measured by RNA-sequencing. Total of 175 differentially expressed lncRNAs (DELs), 1321 differentially expressed mRNAs (DEMs) and 94 differentially expressed microRNAs (DEMIs) were identified in stage I LUAD. DEMI-DEM regulatory network consisted of 544 nodes and 1123 edge; miR-200 family members had high connectivity with DEMs. In DEL-DEM co-expression network, CDKN2B-AS1, FENDRR and LINC00312 had the high connectivity with DEMs, which co-expressed with 105, 63 and 61 DEMs, respectively. DEL-DEMI-DEM network depicted the links among DELs, DEMI and DEMs. Identified DEMs were significantly enriched in cell adhesion molecules, focal adhesion and tight junction of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways; and enriched in cell adhesion, angiogenesis and regulation of cell proliferation of Gene Ontology biological processes. Quantitative real-time polymerase chain reaction results were generally consistent with our bioinformatics analyses. LINC00312 and FENDRR had diagnostic value for LUAD patients in The Cancer Genome Atlas database. Our study might lay the foundation for illumination of pathogenesis of LUAD and identification of potential therapeutic targets and novel diagnosis biomarkers for LUAD patients.

Identification of ceRNA network based on a RNA‑seq shows prognostic lncRNA biomarkers in human lung adenocarcinoma

“…However, the study of lncRNA expression profiles in LUAD has gradually increased. Using TNM I stage lung adenocarcinoma samples in the TCGA database, Tian et al ( 40 ) demonstrated that FENDRR and LINC00312 had diagnostic value in patients with LUAD. A recent study has demonstrated that lncRNA can act as a ceRNA that adsorbs onto miRNAs to regulate mRNA expression ( 41 ).…”

Survival analysis and functional annotation of long non‐coding RNAs in lung adenocarcinoma

“…It is reported that JAM2 is down‐regulated in NSCLC and LUAD . Also, Tian et al demonstrated that JAM2, ADARB1, FENDRR and some other LUAD DEGs might synergistically function in the tumourigenesis of stage I LUAD . Furthermore, Glen et al reported that JAM2 could be targeted for the treatment of NSCLC .…”

Diagnostic and Prognostic Potential of Circulating Long Non-Coding RNAs in Non Small Cell Lung Cancer

“…For instance, MINCR and LBX2-AS1 were up-regulated in RNAsequencing and TCGA dataset, while the results of qRT-PCR showed down-regulation of both lncRNAs. HNF1A-AS1 was significantly down-regulated in RNA-sequencing and qRT-PCR, but was up-regulated in TCGA dataset [32].…”

“…Using qRT-PCR, RNA-SEQ and the TCGA dataset, Tian and his colleges [32] aimed to generate a histopathology relevant lncRNAs for scanning of functional lncRNAs in 6 TNM I stage (stage I) LUAD. They found that the expression of LINC00963, NR2F2-AS1, LINC00515, LINC00162, LINC00312, MGC27382, LINC00472 and FENDRR were significantly dysregulated in LUAD tissues.…”