Identification of DUF538 cDNA clone from Celosia cristata expressed sequences of nonstressed and stressed leaves

Gholizadeh, Ashraf; Kohnehrouz, Bahram Baghban

doi:10.1134/s1021443710020123

Cited by 13 publications

(14 citation statements)

References 16 publications

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“…A switchgrass DUF538 domain containing protein (ortholog of Os01g11240) expressed at very low level under normal condition was strongly induced by heat stress (56.7-fold). The function of DUF538 protein is unknown, however it has been proposed as putative candidate for the common stress related proteins in the plant system [ 65 ]. Exogenously applying maltose-binding fusion protein (MBP-DUF538) on the leaves of tobacco can elevate activities of redox enzymes including catalase, peroxidase, polyphenol oxidase and phenyalanine ammonia lyase [ 66 ].…”

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis of heat stress response in switchgrass (Panicum virgatumL.)

Wang

Tang

et al. 2013

BMC Plant Biol

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BackgroundGlobal warming predictions indicate that temperatures will increase by another 2-6°C by the end of this century. High temperature is a major abiotic stress limiting plant growth and productivity in many areas of the world. Switchgrass (Panicum virgatum L.) is a model herbaceous bioenergy crop, due to its rapid growth rate, reliable biomass yield, minimal requirements of water and nutrients, adaptability to grow on marginal lands and widespread distribution throughout North America. The effect of high temperature on switchgrass physiology, cell wall composition and biomass yields has been reported. However, there is void in the knowledge of the molecular responses to heat stress in switchgrass.ResultsWe conducted long-term heat stress treatment (38°/30°C, day/night, for 50 days) in the switchgrass cultivar Alamo. A significant decrease in the plant height and total biomass was evident in the heat stressed plants compared to controls. Total RNA from control and heat stress samples were used for transcriptome analysis with switchgrass Affymetrix genechips. Following normalization and pre-processing, 5365 probesets were identified as differentially expressed using a 2-fold cutoff. Of these, 2233 probesets (2000 switchgrass unigenes) were up-regulated, and 3132 probesets (2809 unigenes) were down-regulated. Differential expression of 42 randomly selected genes from this list was validated using RT-PCR. Rice orthologs were retrieved for 78.7% of the heat stress responsive switchgrass probesets. Gene ontology (GOs) enrichment analysis using AgriGO program showed that genes related to ATPase regulator, chaperone binding, and protein folding was significantly up-regulated. GOs associated with protein modification, transcription, phosphorus and nitrogen metabolic processes, were significantly down-regulated by heat stress.ConclusionsPlausible connections were identified between the identified GOs, physiological responses and heat response phenotype observed in switchgrass plants. Comparative transcriptome analysis in response to heat stress among four monocots – switchgrass, rice, wheat and maize identified 16 common genes, most of which were associated with protein refolding processes. These core genes will be valuable biomarkers for identifying heat sensitive plant germplasm since they are responsive to both short duration as well as chronic heat stress treatments, and are also expressed in different plant growth stages and tissue types.

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Section: Resultsmentioning

confidence: 99%

Transcriptome analysis of heat stress response in switchgrass (Panicum virgatumL.)

Wang

Tang

et al. 2013

BMC Plant Biol

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“…DUF538 domain containing protein is known to activate redox system and stress response. [75] The mRNA expression corroborating with protein levels of DUF538 showed increased abundance at early stage and decrease during later stage of infection in cv. Kufri Chipsona-1.…”

Section: Redox Dependent Cell Death and Necrosismentioning

confidence: 68%

Molecular Dissection of Extracellular Matrix Proteome Reveals Discrete Mechanism Regulating Verticillium Dahliae Triggered Vascular Wilt Disease in Potato

et al. 2017

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Plants exposed to patho-stress mostly succumb due to disease by disruption of cellular integrity and changes in the composition of the extracellular matrix (ECM). Vascular wilt, caused by the soil borne hemibiotrophic filamentous fungus Verticillium dahliae, is one of the most significant diseases that adversely affect plant growth and productivity. The virulence of the pathogen associated with the ECM-related susceptibility of the host plant is far from being understood. To better understand ECM-associated disease responses that allow the pathogen to suppress plant immunity, a temporal analysis of ECM proteome was carried out in vascular wilt susceptible potato cultivar upon V. dahliae infection. The proteome profiling led to the identification of 75 patho-stress responsive proteins (PSRPs), predominantly involved in wall hydration, architecture, and redox homeostasis. Two novel clues regarding wilt disease of potato were gained from this study. First, wall crosslinking and salicylic acid signaling significantly altered during patho-stress. Second, generation of reactive oxygen species and scavenging proteins increased in abundance leading to cell death and necrosis of the host. We provide evidence for the first time that how fungal invasion affects the integrity of ECM components and host reprogramming for susceptibility may function at the cell surface by protein plasticity.

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“…The expression of Celosia leaf DUF5538 gene has been previously examined under drought stress conditions by using basic RT-PCR method through a time-course experiment (Gholizadeh and BaghbanKohnehrouz 2010). In this work, parallel to the chlorophyll assays, the presence and the level of DUF538 gene expression was analyzed in 3 different drought-stressed leaves of Celosia plant using real time gene expression method.…”

Section: Duf538 Gene Expression Is Relevant To Chlorophyll Losementioning

confidence: 99%

“…DUF538 domain containing proteins have been mostly identified using genome annotation tools and cloned as induced genes from plants challenged with various environmental stresses such as nutrient deficiency, crown gall, mixed elicitors, and mild drought (Gholizadeh and Baghbankohnehrouz 2010). On the basis of the high phosphorylation potential, DUF538 proteins have been predicted to play important regulatory roles in different stress-challenged plants (Nakagami et al 2010).…”

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confidence: 99%

DUF538 protein superfamily is predicted to be chlorophyll hydrolyzing enzymes in plants

Gholizadeh

2015

Physiol Mol Biol Plants

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The possible hydrolytic activity towards chlorophyll molecules was predicted for DUF538 protein superfamily in plants. It was examined by using computational as well as experimental tools including in vitro chlorophyll degradation, antioxidant compounds production and in vivo real-time gene expression tests.Comparison of the computational data with the experimental results indicated that DUF538 proteins might be chlorophyll hydrolyzing enzyme (most probably carboxyesterase) which degrade chlorophyll molecules (66 % per 12 hrs) to produce new compounds (1.8 fold per 12 hrs) with antioxidant properties. The relevance of DUF538 gene expression level with the chlorophyll contents (2.8 fold increase per chlorophyll content of 50 %) of the drought-stressed leaves showed that chlorophyll degradation by DUF538 is most probably induced in response to stress stimuli. Despite membranous chlorophyll catabolic pathways, DUF538-dependent reactions is predicted to be occurred in the cytosol of the under stressed plants. We addressed as to whether chlorophyll breakdown to antioxidant compounds by DUF538 is a defense mechanism of plants against stress stimuli, in vivo? This question is going to be investigated in our next research project.

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Identification of DUF538 cDNA clone from Celosia cristata expressed sequences of nonstressed and stressed leaves

Cited by 13 publications

References 16 publications

Transcriptome analysis of heat stress response in switchgrass (Panicum virgatumL.)

Transcriptome analysis of heat stress response in switchgrass (Panicum virgatumL.)

Molecular Dissection of Extracellular Matrix Proteome Reveals Discrete Mechanism Regulating Verticillium Dahliae Triggered Vascular Wilt Disease in Potato

DUF538 protein superfamily is predicted to be chlorophyll hydrolyzing enzymes in plants

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