Molecular Dissection of Extracellular Matrix Proteome Reveals Discrete Mechanism Regulating Verticillium Dahliae Triggered Vascular Wilt Disease in Potato

Abstract

Plants exposed to patho-stress mostly succumb due to disease by disruption of cellular integrity and changes in the composition of the extracellular matrix (ECM). Vascular wilt, caused by the soil borne hemibiotrophic filamentous fungus Verticillium dahliae, is one of the most significant diseases that adversely affect plant growth and productivity. The virulence of the pathogen associated with the ECM-related susceptibility of the host plant is far from being understood. To better understand ECM-associated disease responses that allow the pathogen to suppress plant immunity, a temporal analysis of ECM proteome was carried out in vascular wilt susceptible potato cultivar upon V. dahliae infection. The proteome profiling led to the identification of 75 patho-stress responsive proteins (PSRPs), predominantly involved in wall hydration, architecture, and redox homeostasis. Two novel clues regarding wilt disease of potato were gained from this study. First, wall crosslinking and salicylic acid signaling significantly altered during patho-stress. Second, generation of reactive oxygen species and scavenging proteins increased in abundance leading to cell death and necrosis of the host. We provide evidence for the first time that how fungal invasion affects the integrity of ECM components and host reprogramming for susceptibility may function at the cell surface by protein plasticity.

Overexpression of DUF538 from Wild Arachis Enhances Plant Resistance to Meloidogyne spp.

“…Regarding biotic stresses, some reports identified DUF538 transcripts or proteins in response to infection by fungal pathogens [13][14][15][16], where they seem to be important activators of the plant antioxidative system and a common component in the responses of different stress-challenged plants [17][18][19]. Additionally, a study of grapevine (Vitis vinifera) showed that genes encoding sugar transporters containing a DUF538 domain, such as SWEET17 proteins, are significantly upregulated in leaves infected with phytoplasma [20].…”

Proteo-metabolomic Dissection of Extracellular Matrix Reveals Alterations in Cell Wall Integrity and Calcium Signaling Governs Wall-Associated Susceptibility during Stem Rot Disease in Jute

“…Therefore, unraveling the repertoire of extracellular matrix proteins for managing rot fungus would offer insight into key processes that regulate disease or immune response. Various studies to date reported the ECM proteome of plants in response to hemibiotrophic fungal pathogens, particularly Fusarium and Verticillium , and highlighted the role of ECM proteins in disease and defense pathways. − Also, Fusarium verticillioides elicitor-induced changes were reported in the maize extracellular matrix proteome . In this study, we investigated the stem rot responsive temporal ECM proteome using (isobaric tags for relative and absolute quantitation) iTRAQ based quantitative proteomics to understand wall mediated changes triggering disease responses in a jute variety in response to M. phaseolina attack.…”