Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay

Subramanian, V.; Gurtu, S; Rao, R. C. Nageswara; Nigam, S. N.

doi:10.1139/g00-034

Cited by 81 publications

(59 citation statements)

References 19 publications

(23 reference statements)

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“…Cultivars S 230 and Spanish Improved derived as selections from germplasm were in different group as per quantitative traits, but at genetic level they were similar. As observed in the present study also very low diversity among cultivars at genetic level have been reported by using RAPD (Subramanian et al, 2000 ;Dwivedi et al, 2001), AFLP (Herselman, 2003) and SSR (Raina et al, 2001;Krishna et al, 2004) markers.…”

Section: Morphological Vs Molecular Diversitysupporting

confidence: 53%

“…Banding pattern of seventeen genotypes is presented in Figure 5. High polymorphism observed among the cultivars may be due to use of reported polymorphic primers (Subramanian et al, 2000;Dwivedi et al, 2001;Rajendraprasad, 2003;Vijay, 2003;Nagaraja Reddy et al, 2004;Pattanashetti, 2005). In contrast, Dwivedi et al, 2001 have reported low to moderate level of polymorphism ranging from 8.7 to 33.1 per cent among primers in twenty-six accessions including interspecific derivatives, land races and released cultivars.…”

Section: Molecular Diversitymentioning

confidence: 99%

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Improving Genetic Attributes of Confectionary Traits in Peanut (Arachis hypogaea L.) Using Multivariate Analytical Tools

Ajay¹,

Gowda²,

Rathnakumar³

et al. 2011

JAS

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Peanut is gaining importance more for its confectionery and nutritive values than for its oil content around the world. Improving confectionery qualities is an added advantage for farming community. Hence, in the present study, multivariate analytical tools were used to identify parents with complementary traits for using them in breeding programme. PCA revealed contribution of pod yield, 100-seed weight, oil content, and O/L ratio towards variance. Pod yield was positively associated with 100-seed weight, oil and protein contents. Oil content had weak association with protein content, oleic acid and O/L ratio. UPGMA clustering revealed grouping of cultivars based on origin and its area recommendation. Cultivars superior for yield (GPBD-4, M-28-2 and JL 24) and confectionery traits (S-230 and Dh-8) were identified. Strong positive relation of yield with confectionery traits indicates possibility of breeding high yielding confectionery grade cultivars. Multivariate analytical tools could be used to identify parents for location specific breeding for improvement of Confectionary traits.

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Section: Morphological Vs Molecular Diversitysupporting

confidence: 53%

Section: Molecular Diversitymentioning

confidence: 99%

Improving Genetic Attributes of Confectionary Traits in Peanut (Arachis hypogaea L.) Using Multivariate Analytical Tools

Ajay¹,

Gowda²,

Rathnakumar³

et al. 2011

JAS

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“…This would compliment classical breeding programmes and provide the basis for map-based cloning for groundnut rosette resistance genes. However, results throughout the world (Grieshammer and Wynne 1990;Halward et al 1991;He and Prakash 1997;Hopkins et al 1999;Subramanian et al 2000;Herselman 2003) have indicated that molecular work on cultivated peanut presents a significant challenge due to its inherent narrow genetic base.…”

Section: Introductionmentioning

confidence: 99%

Identification and mapping of AFLP markers linked to peanut (Arachis hypogaea L.) resistance to the aphid vector of groundnut rosette disease

et al. 2004

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Groundnut rosette disease is the most destructive viral disease of peanut in Africa and can cause serious yield losses under favourable conditions. The development of disease-resistant cultivars is the most effective control strategy. Resistance to the aphid vector, Aphis craccivora, was identified in the breeding line ICG 12991 and is controlled by a single recessive gene. Bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) analysis were employed to identify DNA markers linked to aphid resistance and for the development of a partial genetic linkage map. A F(2:3) population was developed from a cross using the aphid-resistant parent ICG 12991. Genotyping was carried out in the F2 generation and phenotyping in the F3 generation. Results were used to assign individual F2 lines as homozygous-resistant, homozygous-susceptible or segregating. A total of 308 AFLP (20 EcoRI+3/MseI+3, 144 MluI+3/MseI+3 and 144 PstI+3/MseI+3) primer combinations were used to identify markers associated with aphid resistance in the F(2:3) population. Twenty putative markers were identified, of which 12 mapped to five linkage groups covering a map distance of 139.4 cM. A single recessive gene was mapped on linkage group 1, 3.9 cM from a marker originating from the susceptible parent, that explained 76.1% of the phenotypic variation for aphid resistance. This study represents the first report on the identification of molecular markers closely linked to aphid resistance to groundnut rosette disease and the construction of the first partial genetic linkage map for cultivated peanut.

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“…The first studies were based on isozymes and proteins (Krishna and Mitra 1988;Grieshammer and Wynne 1990;Lu and Pickersgill 1993), followed by Restriction Fragment Length Polymorphism-RFLPs Paik-Ro et al 1992;Kochert et al 1996), Random Amplified Polymorphic DNARAPDs (Halward et al , 1992Hilu and Stalker 1995;Subramanian et al 2000), Amplified Fragment Length Polymorphism-AFLPs (He and Prakash, 1997;Gimenes et al 2000;He and Prakash 2001;Gimenes et al 2002;Herselman, 2003;Milla et al 2005aMilla et al , 2005bTallury et al 2005), and more recently microsatellite markers (Hopkins et al 1999;Palmieri et al 2002;He et al 2003;Ferguson et al 2004;Moretzsohn et al 2004;He et al 2005;Moretzsohn et al 2005;Palmieri et al 2005;Bravo et al 2006;Budiman et al 2006;Gimenes et al 2007;Proite et al 2007;Wang et al 2007;Cuc et al 2008;Naito et al 2008;Liang et al 2009;Moretzsohn et al 2009;Song et al 2010;Yuan et al 2010;Koilkonda et al 2012;Macedo et al 2012;Pandey et al 2012) and...…”

Section: Molecular Markers For Arachismentioning

confidence: 99%

“…Screening of isozyme, RFLP, and RAPD markers on accessions of A. hypogaea identified only very low levels of polymorphism among cultivated peanut accessions Halward et al 1992;Lu and Pickersgill 1993;Burow et al 1996;Subramanian et al 2000;Dwivedi et al 2001). The partial first linkage map from a cross between accessions of A. hypogaea was constructed using an F 2 population (Herselman et al 2004).…”

Section: Genetic Maps Based On Cultivated X Cultivated Crossesmentioning

confidence: 99%

Marker‐Assisted Selection for Biotic Stress Resistance in Peanut

Burow¹,

Leal‐Bertioli²,

Simpson³

et al. 2013

Translational Genomics for Crop Breeding

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Peanut is the second-most important legum e grown worldwide. Cultivated peanut is a disomic tetraploid, 2n-4 x -40, with limited genetic diversity due to a genetic bottleneck in formation o f the polyploid from ancestors A. duranensis and A. ipaensis. Consequently, resistance_to biotic stresses is limited in the cultigen; however, w ild species possess strong resistances. Transfer o f these re sistances is hindered by differences o f ploidy, but production o f synthetic amphidiploids, coupled with use o f m olecular markers, enables efficient gene transfer. Marker maps have been made from interspecific crosses, and SSR-based maps from cultivated parents have been developed recently. At least 410 resistance gene analogues have been identified. The first markers for biotic stress tolerance were for root-knot nematode resistance and introgressed from one A. cardenasii chromosome. These and improved markers have been used for marker-assisted backcrossing, contributing to release o f three cultivars. Additional QTLs have been identified since.Early and late leafspots cause significant yield losses worldwide, and resistance depends on multiple genes. U sing interspecific populations, five resistance QTLs for early leafspot were identified using greenhouse inoculations, and five QTLs for late leafspot were identified using detached leaf assays. U sing cultivated species populations, 28 QTLs were identified for LLS resistance; all but one w ere minor QTLs; the major QTL was donated by an interspecific introgression line parent. Rust often occurs alongside leafspots, and rust resistance was characterized as one major QTL, plus several smaller QTLs. Marker-assisted backcrossing o f this major QTL has been performed into different populations. QTLs for resistance to other biotic stresses have been identified, namely to groundnut rosette virus, Sclerotinia blight, afiatoxin contamination, aphids, and tomato spotted w ilt virus. Marker-assisted breeding is still in early stages, and develop ment o f more rapid and inexpensive markers from transcriptome and genom e sequencing is expected to accelerate progress.

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Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay

Cited by 81 publications

References 19 publications

Improving Genetic Attributes of Confectionary Traits in Peanut (Arachis hypogaea L.) Using Multivariate Analytical Tools

Improving Genetic Attributes of Confectionary Traits in Peanut (Arachis hypogaea L.) Using Multivariate Analytical Tools

Identification and mapping of AFLP markers linked to peanut (Arachis hypogaea L.) resistance to the aphid vector of groundnut rosette disease

Marker‐Assisted Selection for Biotic Stress Resistance in Peanut

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