Identification of Differentially Expressed Genes During the Brachyurization of the Chinese Mitten Crab Eriocheir japonica sinensis

Abstract

Expressed sequence tags (ESTs) obtained from two-directional suppression subtractive hybridization (SSH) of cDNA libraries derived from the pleon of the Chinese mitten crab were analyzed using mRNA subtractive hybridization. After cDNA cloning and sequencing, gene expression profiles were examined, focusing on brachyurization. We assembled 211 non-redundant ESTs from SSH library 1 (yielding 130 unique transcripts: 25 consensi, 105 singletons) and 669 from SSH library 2 (195: 51 consensi, 144 singletons). Functional analysis shows that these genes are putatively involved in various cellular processes (33%), ribosomal RNA/proteins (30%), molting (12%), signal transduction (6%), immune factor and stress proteins (6%), development (4%), energy metabolism (3%), and mitochondrial membrane protein, hormone metabolizing, and chaperone (1% each) of other organisms. Some 3% are of unknown function in SSH library 1. The results facilitate the functional study of candidate genes involved in early developmental processes, especially the regulation of brachyurization in this crab.

Identification of an Alpha-Tubulin Gene from the Chinese Mitten Crab Eriocheir sinensis: Expression Profiles under Immune Challenge and during Larval Development

“…Based on ESTs (355 base pairs (bp), dbEST_Id 62013217 and GenBank accession number GE329293; 167 bp, dbEST_Id 62013221 and GenBank accession number GE329297) sequence information obtained from a subtractive hybridization cDNA Library of E. sinensis [26]. New gene-specific primers (Table 1) were designed and synthesized to obtain full-length cDNA sequence.…”

Screening and identification of microRNAs during larval metamorphic development of Chinese mitten crab Eriocheir sinensis

“…Previous studies on brachyurization metamorphosis have determined the morphological and physiological processes, while the molecular mechanisms remain poorly understood (Li, Zha, Sun, Song, & Zhou, 2011;Song et al, 2015). To date, numerous immune-associated miRNAs have been identified in E. sinensis (Ou et al, 2012;Wang et al, 2011), Daphnia pulex (Wheeler et al, 2009) and Marsupenaeus japonicas , but there have been very few reports on the regulated functions of miRNAs in the larval metamorphosis of brachyuran decapod crustaceans.…”