Screening and identification of microRNAs during larval metamorphic development of Chinese mitten crab Eriocheir sinensis

Abstract

MicroRNAs (miRNAs) represent a class of endogenous small non-coding RNAs with 18-25 nucleotides that are involved in diverse biological processes such as organogenesis (Lu et al., 2005), haematopoiesis, behaviour and innate/adaptive immunity (Leaman et al., 2005), cellular proliferation and differentiation (Hwang & Mendell, 2007), gene transcription and post-transcriptional processing (Diederichs & Haber, 2007), individual growth and development (Liu et al., 2009), embryo formation (Kocamis et al., 2013), epigenetics and immune regulation (Rusek et al., 2015).Similar to other types of molecules that regulate gene expression, many miRNAs vary significantly in different tissues and developmental stages, with highly conserved, temporal and tissue-spe-

Eriocheir sinensis feminization-1c (Fem-1c) and Its Predicted miRNAs Involved in Sexual Development and Regulation

“…The PC-3p-795 targeted Ras-related protein acted on the Wnt pathway. Our results agree with the analysis of miRNAs during larval metamorphic and gonadal development in E. sinensis [31].…”

“…The miR-17-3p is highly regulated during spermatogonia differentiation and development in E. sinensis [30]. The miR-305-5p, miR-263a-5p, and miR-7-5p play major regulatory roles in the metamorphosis of E. sinensis [31]. In E. sinensis, miR-34 and let-7b can down-regulate insulin-like androgenic gland hormone (IAG) gene expression, whereas miR-9-5p, let-7, and miR-8915 can down-regulate Double-sex (Dsx) gene expression [32].…”

Identification of differentially expressed genes and signalling pathways to elucidate molecular mechanisms underlying growth differences among the male morphotypes of Macrobrachium rosenbergii

“…The housekeeping gene GAPDH was used as the internal control, and all reactions were performed in biological triplicates to reduce experiment errors. The relative expression ratio of target genes versus the GAPDH gene was determined using the 2 −ΔΔCT method (Chen et al., 2020).…”

Transcriptional Landscape of Small Non-coding RNAs in Somatic and Gonadal Tissues of Brachyuran Crabs (Genus Eriocheir and Portunus)

“…The well-known function of miRNAs it the regulation of gene expression, which is involved in immune system, differentiation and proliferation, growth and development, tumorigenesis, and cell death (O'Connell et al, 2010). To date, miRNAs have been identified and characterized in several crab species including Chinese mitten crab Eriocheir sinensis (Song et al, 2014;He et al, 2015;Chen et al, 2020;Fu et al, 2021;Luo et al, 2021), mud crab Scylla paramamosain (Li et al, 2013;Jia et al, 2018;Wang et al, 2018;Waiho et al, 2019;Jin et al, 2020;Lai et al, 2020), swimming crab Portunus trituberculatus (Ren et al, 2016;Meng et al, 2018Meng et al, , 2020, and freshwater crab Sinopotamon henanense (Xu et al, 2019). However, most of the studies often evaluated specific tissue expressed miRNAs in crabs.…”