Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP

Cryopreservation of gametes for aquaculture and alternative cell sources for genome preservation

“…Indeed, most researchers suspect that the DNA reprogramming process taking place on gametic chromatin during normal embryo development (Mhanni and McGowan, 2004) does not operate properly when the chromatin is from somatic origin (Pei et al, 2007a(Pei et al, , 2009Liu et al, 2008;Le Bail et al, 2010;Siripattarapravat et al, 2010). Accordingly, Zhu and collaborators in Wuhan, China, have been working extensively on gene expression in clones, and have identifi ed several genes whose expression is altered in cloned embryos (Pei et al, 2007a(Pei et al, , 2009Liu et al, 2008;Luo et al, 2009Luo et al, , 2011. How the exogenous chromatin can be modifi ed in order to undergo a proper reprogramming upon development initiation is currently the main focus for research in the fi eld.…”

The identification of apolipoprotein C-I in rare minnow (Gobiocypris rarus) and its expression following cadmium exposure

“…Briefly started, reactions were performed in 20 L total reaction volume that contained 10 L 2× SuperReal PreMix, 1 L cDNA template, and 0.5 L of each target-specific primer. The primers for rare minnow ApoC-I were designed from partial fragment gained in the cDNA subtraction library while the primers for ␤-actin were designed from conserved regions of corresponding genes from rare minnow (Liu et al, 2008a). All the primers were listed in Table 1.…”

Cross‐species cloning: influence of cytoplasmic factors on development

“…Although these two types of crossbred embryos originated from common nuclei of the same genetic materials, diverse developmental capacities were gained due to different cytoplasmic environments from different species (Liu et al . ). Using the cDNA amplified fragment length polymorphism (cDNA‐AFLP) approach, we compared transcript profiles between the mutual crossbred embryos at two developmental stages (50%‐ and 90%‐epiboly).…”